Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 75067..75592 | Replicon | plasmid pCRKP18622-2 |
| Accession | NZ_CP027865 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | D4HQE7 |
| Locus tag | C7K64_RS27460 | Protein ID | WP_013023785.1 |
| Coordinates | 75067..75372 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | C7K64_RS27465 | Protein ID | WP_001568025.1 |
| Coordinates | 75374..75592 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C7K64_RS27430 (C7K64_28260) | 70810..71436 | + | 627 | WP_004197649.1 | ParA family plasmid-partitioning AAA ATPase | - |
| C7K64_RS27435 | 71433..71735 | + | 303 | WP_004197636.1 | hypothetical protein | - |
| C7K64_RS27440 (C7K64_28270) | 72143..72937 | - | 795 | WP_004197635.1 | site-specific integrase | - |
| C7K64_RS27445 (C7K64_28275) | 73135..74151 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| C7K64_RS27450 (C7K64_28280) | 74162..74476 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| C7K64_RS27455 (C7K64_28285) | 74503..74898 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| C7K64_RS27460 (C7K64_28290) | 75067..75372 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| C7K64_RS27465 (C7K64_28295) | 75374..75592 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| C7K64_RS27470 (C7K64_28305) | 75762..75962 | - | 201 | Protein_95 | hypothetical protein | - |
| C7K64_RS27475 (C7K64_28310) | 76188..76451 | + | 264 | WP_013023778.1 | hypothetical protein | - |
| C7K64_RS27480 (C7K64_28315) | 76448..77014 | + | 567 | WP_013023777.1 | hypothetical protein | - |
| C7K64_RS27485 (C7K64_28320) | 77045..77539 | + | 495 | WP_013023776.1 | hypothetical protein | - |
| C7K64_RS27490 (C7K64_28325) | 77583..77951 | + | 369 | WP_015344965.1 | hypothetical protein | - |
| C7K64_RS27495 (C7K64_28330) | 77985..78188 | + | 204 | WP_004098931.1 | HHA domain-containing protein | - |
| C7K64_RS27500 (C7K64_28335) | 78237..78494 | + | 258 | WP_017899889.1 | hypothetical protein | - |
| C7K64_RS27505 (C7K64_28340) | 78570..78824 | + | 255 | WP_009653916.1 | hypothetical protein | - |
| C7K64_RS27510 (C7K64_28345) | 78960..79736 | - | 777 | WP_013609504.1 | DUF2971 domain-containing protein | - |
| C7K64_RS27515 (C7K64_28350) | 79977..80300 | - | 324 | WP_017899891.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1C / mph(A) / sul1 / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr | - | 1..111037 | 111037 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11615.30 Da Isoelectric Point: 6.4672
>T100406 WP_013023785.1 NZ_CP027865:c75372-75067 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T100406 NZ_CP027865:c75372-75067 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT100406 WP_001568025.1 NZ_CP027865:c75592-75374 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT100406 NZ_CP027865:c75592-75374 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |