Detailed information of TA system TA100375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 793621..794420
• Replicon: chromosome
• Accession: NZ_CP027852
• Organism: Plesiomonas shigelloides strain MS-17-188

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C7R88_RS03700
• Protein ID: WP_106910866.1
• Coordinates: 793621..794154 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8AQZ3
• Locus tag: C7R88_RS03705
• Protein ID: WP_010863417.1
• Coordinates: 794151..794420 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R88_RS03685	789472..790734	+	1263	WP_106912060.1	ATP-binding protein	-
C7R88_RS03690	790727..791590	+	864	WP_159033690.1	DUF4435 domain-containing protein	-
C7R88_RS03695	792591..793328	+	738	WP_106910865.1	TIR domain-containing protein	-
C7R88_RS03700	793621..794154	-	534	WP_106910866.1	GNAT family N-acetyltransferase	Toxin
C7R88_RS03705	794151..794420	-	270	WP_010863417.1	DUF1778 domain-containing protein	Antitoxin
C7R88_RS03710	794652..795452	-	801	WP_156669244.1	DUF2971 domain-containing protein	-
C7R88_RS03715	795705..796463	+	759	WP_106910867.1	integron integrase	-
C7R88_RS03720	796442..797650	-	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
C7R88_RS03730	797775..798014	+	240	WP_106910868.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	736943..797650	60707
	-	flank	IS/Tn	-	-	796442..797650	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (95-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-81)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20250.63 Da
• Isoelectric Point: 8.8293

>T100375 WP_106910866.1 NZ_CP027852:c794154-793621 [Plesiomonas shigelloides]
VSWSDKFVELDKSKHDRESFYCGESELDTFIQKFALKHMSAGISRTMVLPAKPVLPNNKLPISAFYTIAPSSICRDTLPE
PLAKKLPRYPIPVFLLAQLSVHSMYQGCGLGKVALIKALQYLWQVNSYIRAYAVVVDCLNENAESFYLKYGFEYLCEHNG
RKRLFLPMKTIEQLFNS

Nucleotide

• Length: 534 bp

>T100375 NZ_CP027852:c794154-793621 [Plesiomonas shigelloides]
GTGAGTTGGTCAGACAAATTTGTTGAGTTAGACAAGTCAAAGCATGATAGAGAGTCTTTCTATTGCGGCGAGTCAGAACT
GGATACGTTCATCCAGAAATTTGCATTGAAGCACATGAGCGCGGGTATTAGCCGGACTATGGTTTTACCCGCAAAGCCAG
TCTTACCTAATAACAAACTACCGATTAGCGCATTTTATACGATTGCACCAAGCTCGATTTGTCGTGATACATTACCTGAG
CCATTAGCAAAGAAACTGCCACGCTATCCAATTCCAGTATTTTTATTAGCACAACTTTCCGTCCATAGTATGTATCAAGG
CTGTGGGCTTGGAAAAGTTGCATTGATAAAAGCTCTGCAATATTTGTGGCAAGTGAATAGCTATATACGAGCGTATGCTG
TTGTTGTTGACTGCTTAAATGAGAATGCTGAGTCATTTTATCTGAAATATGGCTTTGAATATCTTTGTGAACATAATGGT
AGAAAACGTTTGTTTTTGCCAATGAAAACGATAGAACAGTTGTTTAACTCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9882.34 Da
• Isoelectric Point: 4.9059

>AT100375 WP_010863417.1 NZ_CP027852:c794420-794151 [Plesiomonas shigelloides]
MATARLDLRIDEEIKAKAEKASALLGLKSLTEYVVRLMDENSTKVIAEHESITVSNDIFDQFIEACDKAKAPNEALKDAL
AFTKEKGIR

Nucleotide

• Length: 270 bp

>AT100375 NZ_CP027852:c794420-794151 [Plesiomonas shigelloides]
ATGGCTACAGCACGTTTAGATCTTCGCATTGATGAGGAAATCAAAGCGAAAGCTGAGAAAGCATCAGCGTTGTTGGGTCT
GAAAAGCCTGACTGAGTATGTTGTTCGTTTAATGGATGAAAACTCAACAAAGGTGATTGCTGAGCATGAAAGCATCACTG
TAAGTAACGACATTTTCGATCAATTCATCGAAGCTTGCGACAAAGCGAAAGCACCTAATGAGGCATTGAAAGACGCGTTA
GCCTTTACGAAAGAGAAGGGTATCCGGTGA

Structures

Antitoxin

• AlphaFold DB: R8AQZ3