Detailed information of TA system TA100148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4469255..4469774
• Replicon: chromosome
• Accession: NZ_CP027746
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K17_RS20035
• Protein ID: WP_081360541.1
• Coordinates: 4469255..4469536 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H3UB71
• Locus tag: C4K17_RS20040
• Protein ID: WP_009044432.1
• Coordinates: 4469526..4469774 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K17_RS20010	4464877..4465149	+	273	WP_081360544.1	hypothetical protein	-
C4K17_RS20020	4465782..4466573	+	792	WP_081360543.1	methyltransferase domain-containing protein	-
C4K17_RS20025	4466889..4467371	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K17_RS20030	4467428..4469077	+	1650	WP_081360542.1	FMN-binding glutamate synthase family protein	-
C4K17_RS20035	4469255..4469536	-	282	WP_081360541.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K17_RS20040	4469526..4469774	-	249	WP_009044432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K17_RS20045	4469871..4470659	-	789	WP_081360540.1	helix-turn-helix transcriptional regulator	-
C4K17_RS20050	4470744..4471742	+	999	WP_081360539.1	bile acid:sodium symporter	-
C4K17_RS20055	4471956..4473719	+	1764	WP_081360538.1	hypothetical protein	-
C4K17_RS20060	4473792..4474253	+	462	WP_009049642.1	YccF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10821.62 Da
• Isoelectric Point: 10.5816

>T100148 WP_081360541.1 NZ_CP027746:c4469536-4469255 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVNPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSVYQQASKR

Nucleotide

• Length: 282 bp

>T100148 NZ_CP027746:c4469536-4469255 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACGGCGGGTTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGTCTATCAGCAAGCCAGCAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8997.37 Da
• Isoelectric Point: 4.4142

>AT100148 WP_009044432.1 NZ_CP027746:c4469774-4469526 [Pseudomonas chlororaphis subsp. aurantiaca]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT100148 NZ_CP027746:c4469774-4469526 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACCCATGTCCTTCTGTCCAACGTCGTTGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAACGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H3UB71