Detailed information of TA system TA100119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2445130..2445715
• Replicon: chromosome
• Accession: NZ_CP027741
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain 449

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K22_RS11315
• Protein ID: WP_124334452.1
• Coordinates: 2445422..2445715 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7ICX6
• Locus tag: C4K22_RS11310
• Protein ID: WP_038580367.1
• Coordinates: 2445130..2445420 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K22_RS11295	2440483..2442963	+	2481	WP_124334449.1	FtsX-like permease family protein	-
C4K22_RS11300	2442953..2444029	+	1077	WP_124334450.1	iron ABC transporter permease	-
C4K22_RS11305	2444143..2444730	+	588	WP_124334451.1	hypothetical protein	-
C4K22_RS11310	2445130..2445420	-	291	WP_038580367.1	putative addiction module antidote protein	Antitoxin
C4K22_RS11315	2445422..2445715	-	294	WP_124334452.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K22_RS11320	2445950..2447344	-	1395	WP_124334453.1	VOC family protein	-
C4K22_RS11325	2447606..2448730	+	1125	WP_124310797.1	diguanylate cyclase	-
C4K22_RS11330	2448824..2450068	+	1245	WP_124334454.1	M20/M25/M40 family metallo-hydrolase	-
C4K22_RS11335	2450315..2450713	+	399	WP_023968692.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10748.26 Da
• Isoelectric Point: 10.0959

>T100119 WP_124334452.1 NZ_CP027741:c2445715-2445422 [Pseudomonas chlororaphis subsp. aurantiaca]
MDYEILQTTVFAQWHTTQRDLRARIAIARRIERASAGNLGDVKNFGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T100119 NZ_CP027741:c2445715-2445422 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACAACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCGTCGGCGGGCAACCTGGGCGATGTGAAAAACTTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAACACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10239.78 Da
• Isoelectric Point: 8.0096

>AT100119 WP_038580367.1 NZ_CP027741:c2445420-2445130 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT100119 NZ_CP027741:c2445420-2445130 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGGCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACTGTGTCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7ICX6