Detailed information of TA system TA100089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6085103..6085692
• Replicon: chromosome
• Accession: NZ_CP027736
• Organism: Pseudomonas chlororaphis subsp. piscium strain PCL1391

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K33_RS27145
• Protein ID: WP_016702674.1
• Coordinates: 6085393..6085692 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K33_RS27140
• Protein ID: WP_053281098.1
• Coordinates: 6085103..6085396 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K33_RS27110	6080183..6080716	+	534	WP_025807757.1	2,4'-dihydroxyacetophenone dioxygenase family protein	-
C4K33_RS27115	6080891..6081685	+	795	WP_025807760.1	SDR family NAD(P)-dependent oxidoreductase	-
C4K33_RS27120	6081864..6082544	+	681	WP_025807762.1	isochorismatase family protein	-
C4K33_RS27125	6082730..6083134	+	405	WP_063433159.1	DoxX family protein	-
C4K33_RS27130	6083341..6083733	+	393	WP_063433160.1	DUF3077 domain-containing protein	-
C4K33_RS27135	6083934..6085097	+	1164	WP_063433161.1	GGDEF domain-containing protein	-
C4K33_RS27140	6085103..6085396	-	294	WP_053281098.1	putative addiction module antidote protein	Antitoxin
C4K33_RS27145	6085393..6085692	-	300	WP_016702674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K33_RS27150	6085866..6086315	+	450	WP_063433162.1	DUF3828 domain-containing protein	-
C4K33_RS27155	6086340..6087350	-	1011	WP_196762739.1	NAD(P)-dependent alcohol dehydrogenase	-
C4K33_RS27160	6087406..6087705	-	300	WP_007924100.1	ArsR family transcriptional regulator	-
C4K33_RS27165	6087772..6088860	-	1089	WP_025807778.1	nitrite reductase, copper-containing	-
C4K33_RS27170	6089087..6089329	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K33_RS27175	6089326..6090213	+	888	WP_053281103.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11221.93 Da
• Isoelectric Point: 10.6086

>T100089 WP_016702674.1 NZ_CP027736:c6085692-6085393 [Pseudomonas chlororaphis subsp. piscium]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T100089 NZ_CP027736:c6085692-6085393 [Pseudomonas chlororaphis subsp. piscium]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAACGGCCTTGCCGGTGACGTGTCACCGGTGGGCGAGGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCTTGGTTCGCAACTTGTCATTCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10584.16 Da
• Isoelectric Point: 6.4627

>AT100089 WP_053281098.1 NZ_CP027736:c6085396-6085103 [Pseudomonas chlororaphis subsp. piscium]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHVRP

Nucleotide

• Length: 294 bp

>AT100089 NZ_CP027736:c6085396-6085103 [Pseudomonas chlororaphis subsp. piscium]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGTCCCTGCGTGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCGTTCAGCGACAAGGGCAACCCGACCTTGAAGACCCTGCTGGCG
GTGATGCGTGCCCTGGGGCTGGACATGACGGCGCGGGTGCATGTCAGGCCGTAG