Detailed information of TA system TA100065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2553216..2553829
• Replicon: chromosome
• Accession: NZ_CP027733
• Organism: Pseudomonas sp. R4-39-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4U3I3Z1
• Locus tag: C4J88_RS11795
• Protein ID: WP_057722035.1
• Coordinates: 2553578..2553829 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J88_RS11790
• Protein ID: WP_124362545.1
• Coordinates: 2553216..2553581 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J88_RS27510	2549219..2550439	+	1221	WP_177412906.1	hypothetical protein	-
C4J88_RS11780	2550649..2552004	+	1356	WP_124366773.1	MATE family efflux transporter	-
C4J88_RS11785	2552044..2553249	+	1206	WP_124407000.1	MFS transporter	-
C4J88_RS11790	2553216..2553581	-	366	WP_124362545.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J88_RS11795	2553578..2553829	-	252	WP_057722035.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9490.04 Da
• Isoelectric Point: 10.0459

>T100065 WP_057722035.1 NZ_CP027733:c2553829-2553578 [Pseudomonas sp. R4-39-08]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100065 NZ_CP027733:c2553829-2553578 [Pseudomonas sp. R4-39-08]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAACTCGTAACCCTGTT
ACGGCGACTGGGTTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAGTGCACTGATCA
CCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13208.01 Da
• Isoelectric Point: 6.7400

>AT100065 WP_124362545.1 NZ_CP027733:c2553581-2553216 [Pseudomonas sp. R4-39-08]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLHHHFPQPCAATG

Nucleotide

• Length: 366 bp

>AT100065 NZ_CP027733:c2553581-2553216 [Pseudomonas sp. R4-39-08]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTGGTAAGCTACGAAGGCAAAACCGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCAGTAGACG
ATTACCTCGCCACCTGCCAGTCGCTCGGGCAAACGCCTGAAAAGCCATGCAAGGGCTCGTTCAACGTACGCGTCGGCCAT
GACCTGCATCTGGCGGCAGCGTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACCCGCCAGGCGCTGGATGA
GTTTCTACACCATCATTTCCCGCAGCCTTGCGCGGCGACCGGCTGA

Structures

Toxin

• AlphaFold DB: A0A4U3I3Z1