TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family higB (T6088) Predicted domain RelE
Locus tag orf339 Length 105 a.a.
Coordinates 374859..375176 (-)

Antitoxin (Protein)


Predicted family higA (AT303) Predicted domain PRK10072 (HTH)
Locus tag orf338 Length 98 a.a.
Coordinates 374504..374800 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf335 371700..372176 - 477 ORF335 Predicted ORF by PRODIGAL Version 1.20 -
orf336 372404..373123 + 720 ORF336 Predicted ORF by PRODIGAL Version 1.20 -
orf337 373120..374472 + 1353 ORF337 Predicted ORF by PRODIGAL Version 1.20 -
orf338 374504..374800 - 297 ORF338 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf339 374859..375176 - 318 ORF339 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf340 375293..376915 - 1623 ORF340 Predicted ORF by PRODIGAL Version 1.20 -
orf341 377294..379018 + 1725 ORF341 Predicted ORF by PRODIGAL Version 1.20 -
orf342 379081..379959 - 879 ORF342 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

RelE (E-value: 1.8e-07, Score:29.3)

(36-100)

Antitoxin

PRK10072 (HTH) (E-value: 7.9e-18, Score:62.8)

(4-95)

TIGR03070 (couple_hipB) (E-value: 0.00054, Score:17.2)

(228-258)

PF13560 (HTH_31) (E-value: 0.0085, Score:13.3)

(23-65)

MqsA_antitoxin (MqsA) (E-value: 0.021, Score:13)

(71-130)

cd00093 (Xre) (E-value: 0.016, Score:12.7)

(29-99)

TIGR03070 (couple_hipB) (E-value: 240, Score:-1.3)

(251-268)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12266.21 Da        Isoelectric Point: 10.2614

>ORF339 TA_6 Toxin
MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTGSFRKIRWNRSGMGKRG
GIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSEKLL

Download         Length: 318 bp

>ORF339 TA_6 Toxin
TTAAAGCAGCTTCTCTGACAATAGTTTCAATGCCTTTCTTTGTTCTTCTGTTAAATCATC
CTGTTCATTTTTAGGATAAATCAACGCCAGATAAATTCGGCCATTGCGTGTTACGTTGTA
ATAAATTATCCGAATGCCACCCCGTTTCCCCATTCCAGAACGGTTCCAGCGAATTTTTCT
AAAACTGCCTGTGCCTGCGATAATGTCACCGGCAGTGGGATCTTGAATGAGTAGTTCCTG
AAAAGCCCGGAACTCATCATCAGGTAACAATAACTGGCGGCGTTTGCTAAATCCTTGTAG
TTCAATAAAGGTGAACAT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11117.78 Da        Isoelectric Point: 5.1891

>ORF338 TA_6 Antitoxin
MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIREATGLRQQDFAVAVGVS
YDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI

Download         Length: 297 bp

>ORF338 TA_6 Antitoxin
TTAGATTGTTTTCAATTGATTGATAATGAAAGGATTCGCTTGCAAAAGCAGGAGCAATTT
TCGAGGTGCTCCGGTAGGCTGACGGCGTTTGGTTTCCCAGCTTTTGACCAGGTCATAACT
TACCCCTACGGCGACAGCAAAATCTTGCTGACGTAAACCCGTTGCTTCACGGATCGCTTT
TACATCGATCTCGCTAAAAGTATGGACGTGCTCTGGTTCAGGCGTTTCTTCACCCTTTTC
GATGCGTACCATTTCTTCTGCGCTGGCAAGAAGATCAGCAAATAATTCATCTTTCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6088 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

43

97.087

0.41
T174 Vibrio cholerae O1 biovar El Tor str. N16961

49

90.909

0.467
T303 Pseudomonas putida KT2440

43.434

95.192

0.41
T978 Synechocystis sp. PCC 6803

38.235

56.667

0.248
Protein Organism Identities (%) Coverage (%) Ha-value
AT303 Pseudomonas putida KT2440

38.889

94.737

0.357
AT6088 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

37.313

79.762

0.255
AT174 Vibrio cholerae O1 biovar El Tor str. N16961

26.804

93.269

0.265
AT1005 Escherichia coli O157:H7 str. Sakai

30.769

47.706

0.163