TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family hicA (T10132) Predicted domain -
Locus tag orf3574 Length 92 a.a.
Coordinates 3792473..3792751 (+)

Antitoxin (Protein)


Predicted family hicB (AT10132) Predicted domain COG1598 (HicB)
Locus tag orf3575 Length 118 a.a.
Coordinates 3792744..3793100 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf3570 3788103..3788378 + 276 ORF3570 Predicted ORF by PRODIGAL Version 1.20 -
orf3571 3788380..3789162 + 783 ORF3571 Predicted ORF by PRODIGAL Version 1.20 -
orf3572 3789152..3790291 + 1140 ORF3572 Predicted ORF by PRODIGAL Version 1.20 -
orf3573 3790275..3792368 + 2094 ORF3573 Predicted ORF by PRODIGAL Version 1.20 -
orf3574 3792473..3792751 + 279 ORF3574 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf3575 3792744..3793100 + 357 ORF3575 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf3576 3793157..3793930 - 774 ORF3576 Predicted ORF by PRODIGAL Version 1.20 -
orf3577 3794116..3794376 + 261 ORF3577 Predicted ORF by PRODIGAL Version 1.20 -
orf3578 3794379..3794657 + 279 ORF3578 Predicted ORF by PRODIGAL Version 1.20 -
orf3579 3794813..3795553 + 741 ORF3579 Predicted ORF by PRODIGAL Version 1.20 -
orf3580 3795524..3796291 - 768 ORF3580 Predicted ORF by PRODIGAL Version 1.20 -
orf3581 3796497..3797075 - 579 ORF3581 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin


No domain identified.


Antitoxin

COG1598 (HicB) (E-value: 0.0054, Score:14.6)

(27-103)

COG3905 (RHH) (E-value: 0.64, Score:8)

(7-40)

COG3905 (RHH) (E-value: 38, Score:2.3)

(48-61)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10186.72 Da        Isoelectric Point: 10.2486

>ORF3574 TA_25 Toxin
MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIKNNNGSRRKFQIGTTKF
STHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE

Download         Length: 279 bp

>ORF3574 TA_25 Toxin
ATGATTAAGGTCAGCGCGCTAGGCTCCCGCCATAGAAAAACATTCGACGATGTAATGACG
GAGCCGCCAAAATCTGGAATTAAATGGGATGATGTTGTTGCGCTCATTAAAGCGGTGGGT
GGAACAATAAAAAATAACAATGGGTCCAGAAGGAAATTTCAGATTGGAACGACCAAATTC
AGTACACATGAGCCACATCCAAAGAATGTGATGGATAAAGGTGCAGTAGCAGGTCTTAAG
GAGTGGTTTGTTAACTGTGTAGGGGTAGATTATGAATAA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13409.12 Da        Isoelectric Point: 4.7875

>ORF3575 TA_25 Antitoxin
MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSIEALYREGESALDEWMA
DCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISKNQLIVDVLEREFSAHM

Download         Length: 357 bp

>ORF3575 TA_25 Antitoxin
ATGAATAAAGCATCGAACATTATCACAATTAAAGGTCAACCAGCCGCAGTGACATTTGAA
GCCGATATCAACGCATTTCGCGGCAAGTTCCTCAATGTAAACGGTTATTGTGACTTTGTA
GCCACAAGCATTGAGGCATTATATCGTGAGGGCGAAAGCGCGCTGGATGAGTGGATGGCG
GATTGTGAAGAGGACGGTATTGCCCCTTTTCGTGAAGAAGAAGAGCAAAAACGCCTGACG
CTTCGTGTTCCTCATCACATTGATTCGCGGCTGACCATCGTCGCCAGACAACATTCCATT
TCAAAAAACCAGCTCATTGTTGATGTCTTAGAACGTGAGTTTTCAGCCCATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10132 Acetobacter pasteurianus strain Ab3

33.333

96.429

0.293
T10150 Escherichia coli strain JIE143 transconjugant Tx143

31.148

74.39

0.207
Protein Organism Identities (%) Coverage (%) Ha-value
AT10132 Acetobacter pasteurianus strain Ab3

35.849

96.364

0.322
AT10150 Escherichia coli strain JIE143 transconjugant Tx143

22.727

95.652

0.212