TAfinder detailed results

Overview

Job ID 9mizKMmBqU
Sequence name Escherichia coli strain DH5alpha chromosome, complete genome.
Predicted type II

Toxin (Protein)


Predicted family higB (T6088) Predicted domain RelE
Locus tag C1467_RS23930 Length 103 a.a.
Coordinates 4635076..4635387 (-)

Antitoxin (Protein)


Predicted family higA (AT6088) Predicted domain PRK10072 (HTH)
Locus tag C1467_RS23925 Length 96 a.a.
Coordinates 4634785..4635075 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS23890 4630272..4630907 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
C1467_RS23895 4630904..4631833 + 930 WP_000027702.1 formate dehydrogenase accessory protein FdhE -
C1467_RS23900 4632196..4633404 - 1209 WP_006250222.1 IS4-like element ISVsa5 family transposase -
C1467_RS23910 4633474..4633743 - 270 WP_158649079.1 hypothetical protein -
C1467_RS23915 4633962..4634180 - 219 WP_001295676.1 CopG family transcriptional regulator -
C1467_RS23925 4634785..4635075 - 291 WP_000356397.1 NadS family protein Antitoxin
C1467_RS23930 4635076..4635387 - 312 WP_000897305.1 hypothetical protein Toxin
C1467_RS23935 4635616..4636524 + 909 WP_001162704.1 alpha/beta hydrolase -
C1467_RS23940 4636588..4637529 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
C1467_RS23945 4637574..4638011 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
C1467_RS23950 4638008..4638880 - 873 WP_000920762.1 virulence factor BrkB family protein -
C1467_RS23955 4638874..4639473 - 600 WP_001315111.1 glucose-1-phosphatase -
C1467_RS23960 4639572..4640357 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

RelE (E-value: 0.00037, Score:18.7)

(41-98)

Antitoxin

PRK10072 (HTH) (E-value: 6.4e-09, Score:34.2)

(37-94)

TIGR03070 (couple_hipB) (E-value: 1.3e-05, Score:22.5)

(199-262)

MqsA_antitoxin (MqsA) (E-value: 5.6e-05, Score:21.3)

(58-125)

cd00093 (Xre) (E-value: 8.6e-05, Score:20.2)

(50-110)

smart00530 (HTH_XRE) (E-value: 0.00073, Score:17.7)

(19-76)

COG1476 (XRE) (E-value: 0.0015, Score:15.5)

(16-54)

YdaS_antitoxin (YdaS) (E-value: 0.011, Score:13.8)

(10-44)

Xre-like-HTH (Xre) (E-value: 0.022, Score:13.3)

(15-53)

PF13560 (HTH_31) (E-value: 0.013, Score:12.7)

(21-57)

COG4197 (YdaS) (E-value: 0.033, Score:12.2)

(16-53)

PF01381 (HTH_3) (E-value: 0.0084, Score:11.9)

(187-236)

COG4197 (YdaS) (E-value: 74, Score:1.5)

(17-41)

Xre-like-HTH (Xre) (E-value: 200, Score:0.6)

(18-40)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>WP_000897305.1 TA_35 Toxin
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAG
VRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTRW*

Download         Length: 312 bp

>WP_000897305.1 TA_35 Toxin
TTACCACCTCGTATTTAACAACCGGAGCATTGCTTTTTCCTGGGGAGACAGATCATCTTT
AATACCCTTACGATAAATAAGCAGTAATCTGATCTCATAATGTTTAACCTGATGAAAGTA
AATAACCCTGACACCAGCACGTTTCCCCTTTCCTCCTGACACCCATCGAACCTTTCTTAA
CCCACCAGTTTCAGGTATTACCTCACCATAGTCAGGATTCAAGGCCAGGAAAAACTGAAA
ACGACTGAATTCATCGTCGGTCAGCAGTTTTTGAACATCTTCAGTAAAAATTTCCGTTTC
GATGAAGAGCAT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>WP_000356397.1 TA_35 Antitoxin
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVD
TLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR*

Download         Length: 291 bp

>WP_000356397.1 TA_35 Antitoxin
CTAACGGTTAAGTGCTTGCAGCACATGTTGCGGATCATTGGCAATCGCTCGCAGCAAAGC
CTTTGCAGGCCCGGTCGGTGAACGTCGCCCCTGCTCCCAGTTACGCAACGTGTCTACGCT
TACAGAAATAAGATCGGCAAATTTAGATTGTGACAACCCTGAAGCGCGGCGTATTTCTTT
GATTTTCATAGCATCAACTTCAAAAGTCCGCGAAGGCTGTCGCGTTCCTTCAATAATTTC
ATTCATCTGGGCCATGCTGTGAGTCAACCGCTCAAAAAGTGCCTTATCCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6088 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

70.874

100

0.702
T303 Pseudomonas putida KT2440

52.427

99.038

0.519
T174 Vibrio cholerae O1 biovar El Tor str. N16961

41.176

92.727

0.404
T169 Vibrio cholerae O1 biovar El Tor str. N16961

31.507

73.737

0.221