TAfinder detailed results

Overview

Job ID 9mizKMmBqU
Sequence name Escherichia coli strain DH5alpha chromosome, complete genome.
Predicted type II

Toxin (Protein)


Predicted family yafQ (T6290) Predicted domain COG3041 (YafQ)
Locus tag C1467_RS19765 Length 92 a.a.
Coordinates 3794379..3794657 (+)

Antitoxin (Protein)


Predicted family dinJ (AT6290) Predicted domain PF04221 (RelB)
Locus tag C1467_RS19760 Length 86 a.a.
Coordinates 3794116..3794376 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS19735 3789152..3790291 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
C1467_RS19740 3790275..3792368 + 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
C1467_RS19745 3792473..3792751 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C1467_RS19750 3792744..3793100 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
C1467_RS19755 3793157..3793930 - 774 WP_000543895.1 C40 family peptidase -
C1467_RS19760 3794116..3794376 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1467_RS19765 3794379..3794657 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1467_RS19770 3794813..3795553 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C1467_RS19775 3795524..3796291 - 768 WP_000333380.1 class II glutamine amidotransferase -
C1467_RS19780 3796497..3797075 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

COG3041 (YafQ) (E-value: 1.4e-36, Score:122.7)

(2-90)

TIGR00053 (YafQ) (E-value: 1e-35, Score:120.3)

(2-89)

YafQ_toxin (YafQ) (E-value: 9.3e-33, Score:110.5)

(2-88)

TIGR02385 (RelE) (E-value: 6.6e-25, Score:85.6)

(2-91)

COG4115 (Txe) (E-value: 0.0017, Score:16.5)

(3-84)

TIGR02116 (Txe) (E-value: 0.015, Score:13.5)

(3-83)

Antitoxin

PF04221 (RelB) (E-value: 1.8e-31, Score:106.1)

(1-87)

COG3077 (RelB) (E-value: 9.5e-31, Score:103.9)

(1-88)

TIGR02384 (DinJ) (E-value: 1.4e-16, Score:58.6)

(5-83)

PRK11235 (RelB) (E-value: 6.5e-08, Score:30.7)

(4-48)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>WP_000615976.1 TA_30 Toxin
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYR
DAHVEPDWILIYKLTDKLLRFERTGTHAALFG*

Download         Length: 279 bp

>WP_000615976.1 TA_30 Toxin
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAA
AAGCGTCATAAGGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCT
TTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATCGC
GATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>WP_000729704.1 TA_30 Antitoxin
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ
SIKNSEAGVDVHKAKDADDLFDKLGI*

Download         Length: 261 bp

>WP_000729704.1 TA_30 Antitoxin
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCG
GACGTACTGGCCGGGATGGGGCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAG
GTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGCCTAATCAATTAACCATTCAA
TCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6290 Escherichia coli BW25113

97.826

100

0.968
T1038 Escherichia coli str. K-12 substr. MG1655

97.826

100

0.968
T10190 Pseudomonas sp. strain ANT_J3

46.316

83.333

0.473
T10095 Clostridium difficile R20291

41.573

92.708

0.398
T6256 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

37.234

97.917

0.376
T665 Streptococcus mutans UA159

38.202

96.739

0.366
T166 Vibrio cholerae O1 biovar El Tor str. N16961

36.782

97.753

0.344
T10181 Aggregatibacter actinomycetemcomitans D11S 1

49.057

60.227

0.28
T6127 Helicobacter pylori 26695

38.636

97.778

0.366
T10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

43.333

90

0.419
T10136 Pseudomonas savastanoi

37.647

80.189

0.344
T10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

42.222

90

0.409
T28 Helicobacter pylori 26695

42

56.818

0.226
T6286 Campylobacter jejuni subsp. jejuni IA3902

31.818

96.703

0.301
T10070 Tetragenococcus halophilus NBRC 12172

53.061

52.128

0.28
T6289 Bifidobacterium longum subsp. longum JDM301

28.261

94.845

0.28
T6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

28.261

94.845

0.28
T10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

35.294

89.474

0.323
T10088 Helicobacter pylori P12

42.308

55.914

0.237
T10108 Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes

42.222

73.77

0.204
T10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

42.222

73.77

0.204
T6239 Lactobacillus rhamnosus ATCC 8530

42.222

73.77

0.204
T6144 Lactobacillus rhamnosus ATCC 8530

42.222

73.77

0.204
T6139 Lactobacillus rhamnosus Lc 705

42.222

73.77

0.204
T6146 Proteus vulgaris

32.258

33.696

0.108
Protein Organism Identities (%) Coverage (%) Ha-value
AT6290 Escherichia coli BW25113

98.837

100

0.977
AT1038 Escherichia coli str. K-12 substr. MG1655

98.837

100

0.977
AT6097 Sinorhizobium meliloti 1021

56.863

50

0.333
AT10190 Pseudomonas sp. strain ANT_J3

38.961

88.506

0.345
AT10136 Pseudomonas savastanoi

35.366

75.229

0.333
AT166 Vibrio cholerae O1 biovar El Tor str. N16961

29.63

88.043

0.276
AT6289 Bifidobacterium longum subsp. longum JDM301

27.907

96.629

0.276
AT10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

31.25

89.888

0.287
AT10181 Aggregatibacter actinomycetemcomitans D11S 1

29.487

86.667

0.264
AT10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

30

89.888

0.276
AT10095 Clostridium difficile R20291

29.885

94.565

0.299
AT6256 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

29.31

63.043

0.195
AT6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

28.736

97.753

0.287
AT6261 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

41.026

32.5

0.184
AT10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

30.667

83.333

0.264
AT6239 Lactobacillus rhamnosus ATCC 8530

30.667

83.333

0.264
AT6144 Lactobacillus rhamnosus ATCC 8530

30.667

83.333

0.264
AT6139 Lactobacillus rhamnosus Lc 705

30.667

83.333

0.264
AT6023 Xenorhabdus nematophila ATCC 19061

37.209

51.19

0.184
AT665 Streptococcus mutans UA159

38.182

61.111

0.241
AT1041 Escherichia coli str. K-12 substr. MG1655

37.209

54.43

0.184
AT10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

28

83.333

0.241
AT6258 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

28.814

62.766

0.195
AT10070 Tetragenococcus halophilus NBRC 12172

24.675

95.062

0.218
AT10048 Mycoplasma mycoides subsp. capri str. GM12

25.806

68.889

0.184
AT6259 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

30.769

40.206

0.138