TAfinder detailed results

Overview

Job ID 9mizKMmBqU
Sequence name Escherichia coli strain DH5alpha chromosome, complete genome.
Predicted type II

Toxin (Protein)


Predicted family hicA (T10132) Predicted domain -
Locus tag C1467_RS19745 Length 92 a.a.
Coordinates 3792473..3792751 (+)

Antitoxin (Protein)


Predicted family hicB (AT10132) Predicted domain COG1598 (HicB)
Locus tag C1467_RS19750 Length 118 a.a.
Coordinates 3792744..3793100 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS19725 3788106..3788378 + 273 WP_001341234.1 flagellar biosynthetic protein FliQ -
C1467_RS19730 3788380..3789162 + 783 WP_001260507.1 flagellar biosynthetic protein FliR -
C1467_RS19735 3789152..3790291 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
C1467_RS19740 3790275..3792368 + 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
C1467_RS19745 3792473..3792751 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin Toxin
C1467_RS19750 3792744..3793100 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1467_RS19755 3793157..3793930 - 774 WP_000543895.1 C40 family peptidase -
C1467_RS19760 3794116..3794376 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ -
C1467_RS19765 3794379..3794657 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ -
C1467_RS19770 3794813..3795553 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C1467_RS19775 3795524..3796291 - 768 WP_000333380.1 class II glutamine amidotransferase -
C1467_RS19780 3796497..3797075 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin


No domain identified.


Antitoxin

COG1598 (HicB) (E-value: 0.0056, Score:14.6)

(27-102)

COG3905 (RHH) (E-value: 0.66, Score:8)

(7-40)

COG3905 (RHH) (E-value: 39, Score:2.3)

(48-61)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10186.72 Da        Isoelectric Point: 10.2486

>WP_000598760.1 TA_29 Toxin
MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIKNNNGSRRKFQIGTTKF
STHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE*

Download         Length: 279 bp

>WP_000598760.1 TA_29 Toxin
ATGATTAAGGTCAGCGCGCTAGGCTCCCGCCATAGAAAAACATTCGACGATGTAATGACG
GAGCCGCCAAAATCTGGAATTAAATGGGATGATGTTGTTGCGCTCATTAAAGCGGTGGGT
GGAACAATAAAAAATAACAATGGGTCCAGAAGGAAATTTCAGATTGGAACGACCAAATTC
AGTACACATGAGCCACATCCAAAGAATGTGATGGATAAAGGTGCAGTAGCAGGTCTTAAG
GAGTGGTTTGTTAACTGTGTAGGGGTAGATTATGAATAA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13409.12 Da        Isoelectric Point: 4.7875

>WP_001030483.1 TA_29 Antitoxin
MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSIEALYREGESALDEWMA
DCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISKNQLIVDVLEREFSAHM*

Download         Length: 357 bp

>WP_001030483.1 TA_29 Antitoxin
ATGAATAAAGCATCGAACATTATCACAATTAAAGGTCAACCAGCCGCAGTGACATTTGAA
GCCGATATCAACGCATTTCGCGGCAAGTTCCTCAATGTAAACGGTTATTGTGACTTTGTA
GCCACAAGCATTGAGGCATTATATCGTGAGGGCGAAAGCGCGCTGGATGAGTGGATGGCG
GATTGTGAAGAGGACGGTATTGCCCCTTTTCGTGAAGAAGAAGAGCAAAAACGCCTGACG
CTTCGTGTTCCTCATCACATTGATTCGCGGCTGACCATCGTCGCCAGACAACATTCCATT
TCAAAAAACCAGCTCATTGTTGATGTCTTAGAACGTGAGTTTTCAGCCCATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10132 Acetobacter pasteurianus strain Ab3

33.333

96.429

0.29
T10150 Escherichia coli strain JIE143 transconjugant Tx143

31.148

74.39

0.204
Protein Organism Identities (%) Coverage (%) Ha-value
AT10132 Acetobacter pasteurianus strain Ab3

35.849

96.364

0.319
AT10150 Escherichia coli strain JIE143 transconjugant Tx143

22.727

95.652

0.21