TAfinder detailed results

Overview

Job ID 9mizKMmBqU
Sequence name Escherichia coli strain DH5alpha chromosome, complete genome.
Predicted type II

Toxin (Protein)


Predicted family hicA (T10189) Predicted domain COG1724 (HicA)
Locus tag C1467_RS12940 Length 58 a.a.
Coordinates 2483743..2483919 (-)

Antitoxin (Protein)


Predicted family hicB (AT10189) Predicted domain PF03681 (UPF0150)
Locus tag C1467_RS12935 Length 138 a.a.
Coordinates 2483281..2483697 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS12915 2478433..2479374 - 942 WP_001251315.1 ABC transporter permease -
C1467_RS12920 2479375..2480388 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C1467_RS12925 2480406..2481551 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C1467_RS12930 2481796..2483202 - 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
C1467_RS12935 2483281..2483697 - 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C1467_RS12940 2483743..2483919 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C1467_RS12945 2484141..2484371 + 231 WP_000494244.1 YncJ family protein -
C1467_RS12950 2484463..2486424 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C1467_RS12955 2486497..2487033 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C1467_RS12960 2487086..2488300 + 1215 WP_001326689.1 BenE family transporter YdcO -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

COG1724 (HicA) (E-value: 0.0059, Score:14.7)

(7-66)

PF07927 (HicA) (E-value: 0.012, Score:13.7)

(20-78)

Antitoxin

PF03681 (UPF0150) (E-value: 2.1e-14, Score:51.7)

(11-88)

COG1598 (HicB) (E-value: 6.7e-10, Score:37.1)

(6-128)

HicB_lk_antitox (HicB) (E-value: 2.4e-08, Score:32.2)

(1-65)

PF13744 (HTH_37) (E-value: 0.0032, Score:15.4)

(38-88)

PRK01381 (E-value: 0.0047, Score:15)

(40-74)

HicB-like_2 (HicB) (E-value: 0.016, Score:13.5)

(12-78)

COG3093 (VapI) (E-value: 0.022, Score:11.7)

(14-64)

PRK09726 (HipB) (E-value: 0.047, Score:11.7)

(10-44)

PF03681 (UPF0150) (E-value: 300, Score:-0.5)

(83-110)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>WP_000813794.1 TA_19 Toxin
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS*

Download         Length: 177 bp

>WP_000813794.1 TA_19 Toxin
TTAACTCAAACCGAGTTGTTTCAGGATTGCTTTACGCAATGGTTCTTTAATCTCATCGCA
GGGGTGACGCGGCATGACACTGCGCCTCCCATGAAACCTGAGTTTCAAATGGTTGCTGCC
ATTCGCTACATCGACGCCCTGAGATTCGAGCCAACGTCTGAACTCGCTTTGTTTCAC

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>WP_001270285.1 TA_19 Antitoxin
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLP
SPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKID
AVQLAAKALGKELSLVMV*

Download         Length: 417 bp

>WP_001270285.1 TA_19 Antitoxin
TTAAACCATCACCAGCGATAACTCTTTGCCAAGCGCCTTTGCCGCGAGCTGGACGGCGTC
GATTTTTGTCGCATGATGCAAGTCAAATAGGCGAGTAATCTCCTGTTTAGGTTTGCCAAT
TCGCCTGGCTAACTCTTGCTGAGTGATTTCTGACTGTAAAAAAGCATTTAACAGCAATAC
CTTAGAGGCGACGCTCAAAGGTACTTCAATAAAGTGATCATGACTATTTAATGGCGAAGG
TAAAGGGATAAGCTCGTTATCTTCAAAATAAAAATCAAATGCGGTCAGTAAAGCATCTTT
TGCCGCTTCCATCGCTTCAGCGACAGTTTCGCCCTGCGTCAACGCTTCAGGGATATCCAC
AAAAGAAACCATATAACCGCCTTCCGGCGCGGGTGTAAGAGTGACGGGATAACGCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10189 Escherichia coli strain DH5alpha

100

100

0.983
T1039 Escherichia coli str. K-12 substr. MG1655

100

100

0.983
T6283 Pseudomonas aeruginosa PA1

47.458

98.333

0.475
Protein Organism Identities (%) Coverage (%) Ha-value
AT10189 Escherichia coli strain DH5alpha

99.275

100

0.986
AT1039 Escherichia coli str. K-12 substr. MG1655

99.275

100

0.986
AT6283 Pseudomonas aeruginosa PA1

30.833

50.209

0.266
AT3593 Acinetobacter baumannii ATCC 17978

28.302

73.611

0.216
AT10168 Streptococcus pneumoniae TIGR4

38.983

39.333

0.165
AT875 Sinorhizobium meliloti 1021

38.462

33.987

0.144
AT6107 Burkholderia pseudomallei K96243

27.273

31.884

0.086