TAfinder detailed results

Overview

Job ID 9mizKMmBqU
Sequence name Escherichia coli strain DH5alpha chromosome, complete genome.
Predicted type II

Toxin (Protein)


Predicted family relE (T1041) Predicted domain PF05016 (ParE)
Locus tag C1467_RS12065 Length 95 a.a.
Coordinates 2312060..2312347 (+)

Antitoxin (Protein)


Predicted family relB (AT1041) Predicted domain PRK11235 (RelB)
Locus tag C1467_RS12060 Length 79 a.a.
Coordinates 2311821..2312060 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS12025 2307183..2307672 + 489 Protein_2212 class I SAM-dependent methyltransferase -
C1467_RS25205 2308150..2308571 + 420 Protein_2213 hypothetical protein -
C1467_RS12040 2309227..2310297 + 1071 Protein_2214 ISNCY family transposase -
C1467_RS25445 2310370..2310519 + 150 WP_011443592.1 protein YdfW -
C1467_RS12050 2310956..2311288 - 333 WP_001301033.1 FlxA-like family protein -
C1467_RS12055 2311491..2311796 - 306 WP_001326990.1 protein YdfV -
C1467_RS12060 2311821..2312060 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C1467_RS12065 2312060..2312347 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C1467_RS12070 2312419..2312574 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C1467_RS12075 2312791..2313042 + 252 WP_000980994.1 protein Rem -
C1467_RS12080 2313109..2313387 + 279 WP_012304870.1 hypothetical protein -
C1467_RS12085 2313389..2314438 + 1050 WP_001265199.1 DUF968 domain-containing protein -
C1467_RS12090 2314452..2315204 + 753 WP_001047135.1 antitermination protein -
C1467_RS25000 2315482..2315571 - 90 WP_120795389.1 hypothetical protein -
C1467_RS12095 2315626..2315838 - 213 WP_000087756.1 cold shock-like protein CspF -
C1467_RS12100 2316139..2316354 + 216 WP_000066484.1 cold shock-like protein CspB -
C1467_RS25325 2316718..2316888 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
C1467_RS12105 2317108..2317323 + 216 WP_000839590.1 phage lysis protein EssD -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

PF05016 (ParE) (E-value: 1.1e-36, Score:123.2)

(1-95)

TIGR02385 (RelE) (E-value: 4.9e-26, Score:89.2)

(1-91)

COG2026 (RelE) (E-value: 2.6e-18, Score:64.1)

(1-87)

ParE-like_toxin (ParE) (E-value: 3e-06, Score:25.2)

(6-80)

Antitoxin

PRK11235 (RelB) (E-value: 3e-32, Score:108.7)

(1-80)

PF04221 (RelB) (E-value: 2.7e-26, Score:89.5)

(4-84)

TIGR02384 (DinJ) (E-value: 5.1e-24, Score:82.4)

(5-83)

COG3077 (RelB) (E-value: 1.2e-15, Score:55.4)

(5-68)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>WP_000323025.1 TA_18 Toxin
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGY
RLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL*

Download         Length: 288 bp

>WP_000323025.1 TA_18 Toxin
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCG
ACGGTACGTGAACAGTTGAAAAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAA
GCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTAAGCTCCGGTCTTCAGGCTAT
CGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>WP_000534858.1 TA_18 Antitoxin
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVE
IVKERLRNPKPVRVTLDEL*

Download         Length: 240 bp

>WP_000534858.1 TA_18 Antitoxin
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTT
GAAAAAATGGGTGTAACTCCTTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGAC
AATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATGAAGATGCTGAACTTGTGGAG
ATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1041 Escherichia coli str. K-12 substr. MG1655

100

100

0.99
T173 Vibrio cholerae O1 biovar El Tor str. N16961

56.383

97.917

0.552
T6132 Escherichia coli

54.255

98.947

0.531
T6131 Escherichia coli

54.255

98.947

0.531
T10166 Xenorhabdus nematophila ATCC 19061

58.065

98.936

0.563
T6130 Erwinia pyrifoliae Ep1/96

50

98.947

0.49
T175 Vibrio cholerae O1 biovar El Tor str. N16961

51.064

98.947

0.5
T6023 Xenorhabdus nematophila ATCC 19061

47.059

89.474

0.25
T10157 Streptococcus suis SC84

33.684

100

0.333
T1729 Acidithiobacillus caldus

28.889

100

0.271
T978 Synechocystis sp. PCC 6803

27.66

78.333

0.271
T6285 Streptomyces cattleya NRRL 8057 = DSM 46488

31.325

95.402

0.271
T10158 Streptococcus suis SC84

26.506

96.512

0.229
T727 Streptococcus pneumoniae R6

30.137

86.905

0.229
T326 Streptococcus pneumoniae TIGR4

30.137

86.905

0.229
T174 Vibrio cholerae O1 biovar El Tor str. N16961

23.611

65.455

0.177
Protein Organism Identities (%) Coverage (%) Ha-value
AT1041 Escherichia coli str. K-12 substr. MG1655

100

100

0.988
AT6023 Xenorhabdus nematophila ATCC 19061

41.667

100

0.438
AT10190 Pseudomonas sp. strain ANT_J3

36.364

50.575

0.2
AT6097 Sinorhizobium meliloti 1021

40.741

52.941

0.275
AT665 Streptococcus mutans UA159

37.778

50

0.213
AT10136 Pseudomonas savastanoi

31.148

55.963

0.238
AT6258 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

38.776

52.128

0.238
AT10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

39.535

47.778

0.213
AT6239 Lactobacillus rhamnosus ATCC 8530

39.535

47.778

0.213
AT6144 Lactobacillus rhamnosus ATCC 8530

39.535

47.778

0.213
AT6139 Lactobacillus rhamnosus Lc 705

39.535

47.778

0.213
AT10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

39.535

47.778

0.213
AT10070 Tetragenococcus halophilus NBRC 12172

27.869

75.309

0.213
AT6290 Escherichia coli BW25113

40

46.512

0.2
AT1038 Escherichia coli str. K-12 substr. MG1655

40

46.512

0.2
AT10181 Aggregatibacter actinomycetemcomitans D11S 1

25

48.889

0.138
AT6261 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

31.507

60.833

0.288
AT6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

27.16

91.011

0.275
AT6260 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

31.818

54.321

0.175
AT10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

31.373

57.303

0.2
AT10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

29.412

57.303

0.188
AT10048 Mycoplasma mycoides subsp. capri str. GM12

25

48.889

0.138
AT166 Vibrio cholerae O1 biovar El Tor str. N16961

25.581

46.739

0.137
AT6289 Bifidobacterium longum subsp. longum JDM301

24.691

91.011

0.25
AT6129 Streptococcus mutans

27.907

45.263

0.15
AT6128 Streptococcus mutans

27.907

45.263

0.15