MGE detailed information
MGE type: Conjugative plasmid MGE length: 136397 bp
Accession: NZ_LR130556 Location: 1..136397 Organism: Escherichia coli strain MS14385 isolate MS14385 Replicon: plasmid 2
Accession: NZ_LR130556 Location: 1..136397 Organism: Escherichia coli strain MS14385 isolate MS14385 Replicon: plasmid 2
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EW040_RS25035 () | 1..978 | + | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| EW040_RS25040 () | 1263..2003 | - | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| EW040_RS25045 () | 2124..2306 | - | 183 | WP_042344623.1 | hypothetical protein | - |
| EW040_RS25055 () | 2649..2956 | - | 308 | Protein_3 | type II toxin-antitoxin system ParD family antitoxin | - |
| EW040_RS25065 () | 3449..4618 | + | 1170 | WP_001072355.1 | hypothetical protein | - |
| EW040_RS25070 () | 4651..4779 | + | 129 | Protein_5 | IS481 family transposase | - |
| EW040_RS25075 () | 4814..5107 | - | 294 | WP_001105060.1 | hypothetical protein | - |
| EW040_RS25080 () | 5213..5488 | - | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| EW040_RS25085 () | 5488..5772 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin Detail |
| EW040_RS25090 () | 6377..7129 | + | 753 | WP_000562172.1 | IclR family transcriptional regulator | - |
| EW040_RS25095 () | 7175..8140 | - | 966 | WP_001022265.1 | 2-keto-3-deoxygluconate transporter | - |
| EW040_RS25100 () | 8173..8553 | - | 381 | WP_000710783.1 | Rid family detoxifying hydrolase | - |
| EW040_RS25105 () | 8578..9468 | - | 891 | WP_001077068.1 | dihydrodipicolinate synthase family protein | - |
| EW040_RS25110 () | 9701..9895 | - | 195 | WP_000796505.1 | hypothetical protein | - |
| EW040_RS25115 () | 9869..10309 | + | 441 | Protein_14 | zinc-binding dehydrogenase | - |
| EW040_RS25120 () | 10440..11318 | + | 879 | WP_000338039.1 | sugar ABC transporter permease | - |
| EW040_RS25125 () | 11308..12195 | + | 888 | WP_001271561.1 | carbohydrate ABC transporter permease | - |
| EW040_RS25130 () | 12206..13030 | + | 825 | WP_000922702.1 | phosphodiesterase | - |
| EW040_RS25135 () | 13036..14109 | + | 1074 | WP_000950177.1 | sn-glycerol-3-phosphate import ATP-binding protein UgpC | VF |
| EW040_RS25140 () | 14102..15412 | + | 1311 | WP_000476108.1 | ABC transporter substrate-binding protein | - |
| EW040_RS25145 () | 15471..15779 | - | 309 | Protein_20 | IS4 family transposase ISEc13 | Transposase |
| EW040_RS25150 () | 15856..16089 | - | 234 | Protein_21 | Tn3 family transposase ISPsy39 | Transposase |
| EW040_RS25155 () | 16111..17307 | - | 1197 | Protein_22 | IS3 family transposase | - |
| EW040_RS25160 () | 17332..18036 | + | 705 | WP_001067834.1 | IS3 family transposase IS1203 | Transposase |
| EW040_RS25165 () | 18094..18624 | - | 531 | Protein_24 | histidine-type phosphatase | - |
| EW040_RS25170 () | 18746..19231 | - | 486 | WP_000874189.1 | hypothetical protein | - |
| EW040_RS25175 () | 19256..19741 | - | 486 | WP_001267177.1 | TlpA family protein disulfide reductase | - |
| EW040_RS25180 () | 19728..20423 | - | 696 | WP_000117262.1 | Lipoprotein-releasing system ATP-binding protein LolD | VF |
| EW040_RS25185 () | 20428..21558 | - | 1131 | WP_000729220.1 | ABC transporter permease | - |
| EW040_RS25190 () | 21548..22831 | - | 1284 | WP_000964653.1 | ABC transporter permease | - |
| EW040_RS25195 () | 22834..24213 | - | 1380 | WP_000119836.1 | DUF2318 domain-containing protein | - |
| EW040_RS25200 () | 24317..24844 | - | 528 | WP_000178050.1 | iron transporter | - |
| EW040_RS25205 () | 24885..26771 | - | 1887 | WP_000118029.1 | FTR1 family iron permease | - |
| EW040_RS25215 () | 27118..27933 | - | 816 | WP_012372823.1 | Na(+)-translocating NADH-quinone reductase subunit C | - |
| EW040_RS25220 () | 28116..28622 | - | 507 | WP_000949452.1 | protein disulfide oxidoreductase | - |
| EW040_RS25225 () | 28612..28770 | - | 159 | WP_000449408.1 | DsbA family protein | - |
| EW040_RS27855 () | 28920..29033 | - | 114 | Protein_36 | IS6 family transposase | - |
| EW040_RS25240 () | 29233..29930 | + | 698 | Protein_37 | IS1 family transposase IS1X2 | Transposase |
| EW040_RS25245 () | 30089..30682 | + | 594 | WP_000428546.1 | tetracyline resistance-associated transcriptional repressor TetC | - |
| EW040_RS25250 () | 30795..32000 | - | 1206 | WP_001089068.1 | Tetracycline resistance protein, class B | ARG |
| EW040_RS25255 () | 32082..32705 | + | 624 | WP_000088605.1 | Tetracycline repressor protein class B from transposon Tn10 | VF |
| EW040_RS25260 () | 32683..33369 | - | 687 | WP_001284954.1 | winged helix-turn-helix domain-containing protein | - |
| EW040_RS25265 () | 33377..33763 | - | 387 | WP_000460651.1 | hypothetical protein | - |
| EW040_RS25270 () | 33756..34019 | - | 264 | WP_049802299.1 | antibiotic biosynthesis monooxygenase | - |
| EW040_RS25275 () | 34016..34707 | - | 692 | Protein_44 | IS1 family transposase IS1R | Transposase |
| EW040_RS25280 () | 34764..35012 | - | 249 | Protein_45 | Tn3 family transposase ISEc63 | Transposase |
| EW040_RS25285 () | 35176..35733 | + | 558 | WP_001235713.1 | Tn3 family transposase TnpR_Tn3 | Transposase |
| EW040_RS25290 () | 35916..36776 | + | 861 | WP_000027057.1 | Beta-lactamase TEM | ARG |
| EW040_RS25295 () | 37002..37181 | + | 180 | WP_000214483.1 | hypothetical protein | - |
| EW040_RS25300 () | 37236..37517 | + | 282 | Protein_49 | CcgAII protein | - |
| EW040_RS25310 () | 37625..38461 | - | 837 | WP_045893453.1 | hypothetical protein | ARG |
| EW040_RS25315 () | 38461..39264 | - | 804 | WP_024261901.1 | Aminoglycoside 3'-phosphotransferase | ARG |
| EW040_RS25325 () | 40647..41351 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| EW040_RS25335 () | 41473..42378 | + | 906 | WP_000219391.1 | hypothetical protein | ARG |
| EW040_RS25340 () | 42375..43613 | + | 1239 | WP_000004159.1 | MFS transporter | - |
| EW040_RS25345 () | 43613..44197 | + | 585 | WP_001137892.1 | TetR family transcriptional regulator | - |
| EW040_RS25360 () | 44690..45454 | - | 765 | WP_001389365.1 | IS6-like element IS6100 family transposase | - |
| EW040_RS27605 () | 45572..45637 | - | 66 | Protein_58 | EAL domain-containing protein | - |
| EW040_RS25370 () | 45681..45986 | - | 306 | WP_000130000.1 | Transcriptional regulator | Relaxase |
| EW040_RS25375 () | 45997..47202 | - | 1206 | WP_000184001.1 | chromate efflux transporter | - |
| EW040_RS25380 () | 47358..47561 | - | 204 | WP_000376616.1 | hypothetical protein | - |
| EW040_RS25390 () | 47689..48528 | - | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| EW040_RS25395 () | 48522..48869 | - | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| EW040_RS25400 () | 49075..49863 | - | 789 | WP_000503573.1 | Streptomycin 3''-adenylyltransferase | ARG |
| EW040_RS25405 () | 49994..50467 | - | 474 | WP_001389366.1 | trimethoprim-resistant dihydrofolate reductase DfrA17 | - |
| EW040_RS25410 () | 50625..51305 | + | 681 | Protein_66 | IS91 family transposase ISAzo26 | Transposase |
| EW040_RS25415 () | 51370..52074 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| EW040_RS25420 () | 52140..52685 | + | 546 | Protein_68 | Tn3 family transposase TnpA_Tn3 | Transposase |
| EW040_RS25425 () | 52721..53136 | - | 416 | Protein_69 | IS1 family transposase | - |
| EW040_RS25430 () | 53385..53786 | - | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| EW040_RS25435 () | 53719..53976 | - | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| EW040_RS25440 () | 54069..54722 | - | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| EW040_RS27610 () | 54820..54960 | - | 141 | WP_001333237.1 | hypothetical protein | - |
| EW040_RS25450 () | 55662..56519 | - | 858 | WP_000130640.1 | incFII family plasmid replication initiator RepA | - |
| EW040_RS25455 () | 56512..56586 | - | 75 | WP_031943482.1 | RepA leader peptide Tap | - |
| EW040_RS27705 () | 56583..56717 | - | 135 | Protein_76 | protein CopA/IncA | - |
| EW040_RS25465 () | 56823..56975 | - | 153 | WP_072258247.1 | replication protein A | - |
| EW040_RS25470 () | 57056..58078 | + | 1023 | WP_000255944.1 | IS21 family transposase IS100kyp | Transposase |
| EW040_RS25475 () | 58075..58857 | + | 783 | WP_001300609.1 | IS21 family transposase IS100kyp | Transposase |
| EW040_RS25480 () | 58926..59036 | - | 111 | Protein_80 | replication protein | - |
| EW040_RS25485 () | 59333..59467 | - | 135 | WP_023144756.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| - () | 59512..59572 | + | 61 | NuclAT_2 | - | - |
| - () | 59512..59572 | + | 61 | NuclAT_2 | - | - |
| - () | 59512..59572 | + | 61 | NuclAT_2 | - | - |
| - () | 59512..59572 | + | 61 | NuclAT_2 | - | - |
| EW040_RS27465 () | 59539..59825 | - | 287 | Protein_82 | DUF2726 domain-containing protein | - |
| EW040_RS25500 () | 60338..60550 | - | 213 | WP_013023861.1 | hypothetical protein | - |
| EW040_RS25505 () | 60681..61241 | - | 561 | WP_000139341.1 | fertility inhibition protein FinO | - |
| EW040_RS25510 () | 61296..62042 | - | 747 | WP_000205718.1 | conjugal transfer pilus acetylase TraX | - |
| EW040_RS25515 () | 62062..67332 | - | 5271 | WP_001617867.1 | Multifunctional conjugation protein TraI | Relaxase |
| EW040_RS25520 () | 67332..69530 | - | 2199 | WP_024623315.1 | type IV conjugative transfer system coupling protein TraD | - |
| EW040_RS25525 () | 69527..70111 | - | 585 | WP_077759813.1 | hypothetical protein | - |
| EW040_RS25530 () | 70508..71239 | - | 732 | WP_000850429.1 | complement resistance protein TraT | - |
| EW040_RS25535 () | 71271..71768 | - | 498 | WP_000605862.1 | entry exclusion protein | - |
| EW040_RS25540 () | 71787..74606 | - | 2820 | WP_001007062.1 | conjugal transfer mating pair stabilization protein TraG | - |
| EW040_RS25545 () | 74603..75976 | - | 1374 | WP_000944328.1 | conjugal transfer protein TraH | - |
| EW040_RS25550 () | 75963..76388 | - | 426 | WP_001348758.1 | F-type conjugal transfer protein TrbF | - |
| EW040_RS25555 () | 76336..76683 | - | 348 | WP_071571857.1 | P-type conjugative transfer protein TrbJ | - |
| EW040_RS25560 () | 76613..77158 | - | 546 | WP_000059829.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| EW040_RS25565 () | 77145..77429 | - | 285 | WP_001617873.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| EW040_RS25570 () | 77546..78243 | - | 698 | Protein_97 | IS1 family transposase IS1G | Transposase |
| EW040_RS25575 () | 78303..78383 | - | 81 | Protein_98 | type IV conjugative transfer system protein TraL | - |
| EW040_RS25580 () | 78388..78750 | - | 363 | WP_000338606.1 | type IV conjugative transfer system pilin TraA | - |
| EW040_RS25585 () | 78784..79011 | - | 228 | WP_001254388.1 | conjugal transfer relaxosome protein TraY | - |
| EW040_RS25590 () | 79099..79776 | - | 678 | WP_001348626.1 | PAS domain-containing protein | - |
| EW040_RS25595 () | 79910..80293 | - | 384 | WP_001151566.1 | relaxosome protein TraM | - |
| EW040_RS25600 () | 80624..81226 | + | 603 | WP_000243712.1 | hypothetical protein | VF |
| EW040_RS25605 () | 81523..82344 | - | 822 | WP_001234469.1 | DUF945 domain-containing protein | - |
| EW040_RS25610 () | 82463..82750 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| EW040_RS25615 () | 82775..82981 | - | 207 | WP_000275859.1 | hypothetical protein | - |
| EW040_RS27710 () | 82894..83229 | - | 336 | WP_197725101.1 | hypothetical protein | - |
| EW040_RS25630 () | 83672..83830 | - | 159 | WP_001312861.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| - () | 83874..84001 | - | 128 | NuclAT_0 | - | - |
| - () | 83874..84001 | - | 128 | NuclAT_0 | - | - |
| - () | 83874..84001 | - | 128 | NuclAT_0 | - | - |
| - () | 83874..84001 | - | 128 | NuclAT_0 | - | - |
| - () | 85443..85545 | - | 103 | NuclAT_1 | - | - |
| - () | 85443..85545 | - | 103 | NuclAT_1 | - | - |
| - () | 85443..85545 | - | 103 | NuclAT_1 | - | - |
| - () | 85443..85545 | - | 103 | NuclAT_1 | - | - |
| EW040_RS25645 () | 85557..86276 | - | 720 | WP_001276232.1 | plasmid SOS inhibition protein A | - |
| EW040_RS25650 () | 86273..86707 | - | 435 | WP_000845940.1 | conjugation system SOS inhibitor PsiB | - |
| EW040_RS25655 () | 86762..88720 | - | 1959 | WP_000117179.1 | ParB/RepB/Spo0J family partition protein | - |
| EW040_RS25660 () | 88786..89019 | - | 234 | WP_000005990.1 | DUF905 family protein | - |
| EW040_RS25665 () | 89082..89621 | - | 540 | WP_000290841.1 | single-stranded DNA-binding protein | - |
| EW040_RS27715 () | 89766..90101 | - | 336 | WP_013023883.1 | hypothetical protein | - |
| EW040_RS25690 () | 90264..90482 | - | 219 | WP_001496291.1 | hypothetical protein | - |
| EW040_RS25695 () | 90508..91071 | - | 564 | WP_001348621.1 | class I SAM-dependent methyltransferase | - |
| EW040_RS25700 () | 91119..92480 | - | 1362 | WP_000170714.1 | DUF3560 domain-containing protein | - |
| EW040_RS25705 () | 92532..92762 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| EW040_RS27615 () | 93276..93524 | + | 249 | WP_071556315.1 | hypothetical protein | - |
| EW040_RS25715 () | 93797..93988 | - | 192 | WP_001027516.1 | hypothetical protein | - |
| EW040_RS25720 () | 93985..94407 | - | 423 | WP_000271753.1 | DUF1380 family protein | - |
| EW040_RS25725 () | 94454..94756 | - | 303 | WP_001671341.1 | antirestriction protein | - |
| EW040_RS25735 () | 95296..96072 | - | 777 | WP_001753849.1 | hypothetical protein | - |
| EW040_RS25740 () | 96123..96557 | - | 435 | WP_000274437.1 | DUF1380 family protein | - |
| EW040_RS25745 () | 96571..96792 | - | 222 | WP_001104873.1 | hypothetical protein | - |
| EW040_RS25750 () | 96793..97476 | - | 684 | WP_000086185.1 | DNA methylase | - |
| EW040_RS25755 () | 97553..97857 | - | 305 | Protein_128 | hypothetical protein | - |
| EW040_RS25760 () | 97861..98763 | - | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
| EW040_RS25775 () | 99630..100601 | - | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| EW040_RS25780 () | 100601..101767 | - | 1167 | WP_000772446.1 | hypothetical protein | VF |
| EW040_RS25785 () | 102355..103110 | - | 756 | WP_024946706.1 | replication initiation protein RepE | - |
| EW040_RS25790 () | 103884..104690 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| EW040_RS25795 () | 104691..104996 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| EW040_RS25800 () | 104998..105216 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| EW040_RS25805 () | 105783..106295 | + | 513 | WP_000151784.1 | hypothetical protein | - |
| EW040_RS25810 () | 106329..107462 | - | 1134 | WP_001542067.1 | DUF3800 domain-containing protein | - |
| EW040_RS25815 () | 107629..108402 | - | 774 | WP_000905949.1 | hypothetical protein | - |
| EW040_RS25820 () | 108415..108915 | - | 501 | WP_000528931.1 | hypothetical protein | - |
| EW040_RS25825 () | 109180..109410 | + | 231 | WP_001261278.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin Detail |
| EW040_RS25830 () | 109407..109823 | + | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | Toxin Detail |
| EW040_RS25835 () | 109868..113662 | - | 3795 | WP_001144732.1 | hypothetical protein | - |
| EW040_RS25840 () | 114043..114273 | + | 231 | WP_001261286.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin Detail |
| EW040_RS25845 () | 114270..114686 | + | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | Toxin Detail |
| EW040_RS25850 () | 114761..116326 | + | 1566 | WP_001128474.1 | AAA family ATPase | - |
| EW040_RS25855 () | 116311..117333 | + | 1023 | WP_000361402.1 | helicase UvrD | - |
| EW040_RS25860 () | 117587..118284 | - | 698 | Protein_147 | IS1 family transposase IS1S | Transposase |
| EW040_RS25870 () | 118640..119554 | + | 915 | WP_000949004.1 | putative periplasmic iron-binding protein | VF |
| EW040_RS25875 () | 119554..120381 | + | 828 | WP_000983710.1 | Manganese transport system ATP-binding protein MntB | VF |
| EW040_RS25880 () | 120378..121235 | + | 858 | WP_001101723.1 | Manganese transport system membrane protein MntB | ARG |
| EW040_RS25885 () | 121232..122089 | + | 858 | WP_000968139.1 | Manganese transport system membrane protein MntB | ARG |
| EW040_RS25890 () | 122323..122580 | - | 258 | Protein_152 | Tn3 family transposase | - |
| EW040_RS25895 () | 122578..122991 | + | 414 | Protein_153 | phosphopyruvate hydratase | - |
| EW040_RS25905 () | 123332..123712 | + | 381 | WP_001318207.1 | fluoride efflux transporter CrcB | - |
| EW040_RS25910 () | 124092..125285 | - | 1194 | WP_000095526.1 | MFS transporter | - |
| EW040_RS25915 () | 125421..127145 | + | 1725 | WP_000602863.1 | N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase | VF |
| EW040_RS25920 () | 127146..128093 | + | 948 | WP_000011908.1 | N(6)-hydroxylysine O-acetyltransferase | VF |
| EW040_RS25925 () | 128093..129835 | + | 1743 | WP_001015721.1 | Aerobactin synthase | VF |
| EW040_RS25930 () | 129832..131109 | + | 1278 | WP_000750130.1 | L-lysine N6-monooxygenase | VF |
| EW040_RS25935 () | 131191..133392 | + | 2202 | WP_000973519.1 | Ferric aerobactin receptor | VF |
| EW040_RS25940 () | 133772..134469 | + | 698 | Protein_161 | IS1 family transposase IS1S | Transposase |
| EW040_RS25945 () | 134649..135162 | - | 514 | Protein_162 | DUF4113 domain-containing protein | - |
| EW040_RS25950 () | 135223..135327 | - | 105 | Protein_163 | AAA family ATPase | - |
| EW040_RS25955 () | 135329..135652 | - | 324 | Protein_164 | IS21 family transposase IS21 | Transposase |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|