MGE detailed information

MGE type: Conjugative plasmid        MGE length: 218262 bp
Accession: NZ_CP059722        Location: 1..218262         Organism: Escherichia coli strain BUCT2930       Replicon: plasmid p1buct2930


Gene structure


The 5 kb flanking regions of this MGE are displayed.

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R14_RS00545 () 168..554 + 387 WP_181728339.1 hypothetical protein -
H1R14_RS00550 () 931..1248 + 318 WP_029698059.1 hypothetical protein -
H1R14_RS00555 () 1299..1706 - 408 WP_000074418.1 hypothetical protein -
H1R14_RS00560 () 1736..2197 - 462 WP_000185304.1 hypothetical protein -
H1R14_RS00565 () 2534..2764 - 231 WP_001572393.1 hypothetical protein -
H1R14_RS00570 () 3426..3659 - 234 WP_015059496.1 hypothetical protein -
H1R14_RS00575 () 3881..4777 - 897 WP_000975182.1 hypothetical protein -
H1R14_RS00580 () 4780..5295 - 516 WP_001572389.1 thermonuclease family protein -
H1R14_RS00585 () 5510..6937 + 1428 WP_000833382.1 DNA cytosine methyltransferase -
H1R14_RS00590 () 6998..7165 + 168 WP_000220758.1 hypothetical protein -
H1R14_RS00595 () 7188..8507 + 1320 WP_000078514.1 DUF1173 family protein -
H1R14_RS00600 () 8787..9992 - 1206 WP_001572381.1 hypothetical protein -
H1R14_RS00605 () 9989..10807 - 819 WP_000193209.1 DUF4942 domain-containing protein -
H1R14_RS00610 () 11450..11830 + 381 WP_001426317.1 hypothetical protein -
H1R14_RS00615 () 11888..12553 + 666 WP_000490638.1 hypothetical protein -
H1R14_RS00620 () 12613..13068 + 456 WP_001572344.1 hypothetical protein -
H1R14_RS00625 () 13110..13346 + 237 WP_000175476.1 hypothetical protein -
H1R14_RS00630 () 13844..14248 + 405 WP_001287392.1 H-NS histone family protein -
H1R14_RS00635 () 14590..14793 - 204 WP_001194555.1 hypothetical protein -
H1R14_RS00640 () 14855..15238 - 384 Protein_20 HNH endonuclease -
H1R14_RS00645 () 15824..16810 + 987 WP_001572342.1 hypothetical protein -
H1R14_RS00650 () 16807..18846 + 2040 WP_032192809.1 ATP-dependent helicase -
H1R14_RS00655 () 19159..19458 - 300 WP_001572440.1 hypothetical protein -
H1R14_RS00660 () 19513..19749 - 237 Protein_24 hypothetical protein -
H1R14_RS00665 () 20203..20628 - 426 WP_001043843.1 hypothetical protein -
H1R14_RS00670 () 20882..21697 - 816 WP_001572439.1 HNH endonuclease -
H1R14_RS00675 () 21710..22120 - 411 WP_000985911.1 hypothetical protein -
H1R14_RS00680 () 22222..22428 - 207 WP_000134171.1 hypothetical protein -
H1R14_RS00685 () 22489..23814 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin Detail
H1R14_RS00690 () 23819..24112 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin Detail
H1R14_RS00695 () 24716..25108 - 393 WP_000341066.1 hypothetical protein -
H1R14_RS00700 () 25481..25822 + 342 WP_001151575.1 hypothetical protein -
H1R14_RS00705 () 25914..26105 + 192 WP_000398480.1 hypothetical protein -
H1R14_RS00710 () 26155..26436 + 282 WP_000077926.1 hypothetical protein -
H1R14_RS00715 () 26999..28240 - 1242 WP_001053338.1 VWA domain-containing protein -
H1R14_RS00720 () 28669..29244 - 576 WP_000301242.1 TerD family protein -
H1R14_RS00725 () 29312..29890 - 579 WP_000116677.1 tellurium resistance membrane protein TerD -
H1R14_RS00730 () 29939..30979 - 1041 WP_000255079.1 tellurium resistance membrane protein TerC -
H1R14_RS00735 () 31002..31457 - 456 WP_000007448.1 tellurite resistance TerB family protein -
H1R14_RS00740 () 31480..32637 - 1158 WP_182410937.1 TerD family protein -
H1R14_RS00745 () 32637..33218 - 582 WP_042634303.1 tellurium resistance TerZ family protein -
H1R14_RS00750 () 33542..34600 + 1059 WP_001035162.1 ATP-grasp domain-containing protein -
H1R14_RS00755 () 34610..35752 + 1143 WP_001285478.1 phosphoribosyltransferase domain-containing protein -
H1R14_RS00760 () 35745..36518 + 774 WP_001040059.1 hypothetical protein -
H1R14_RS00765 () 36520..37599 + 1080 WP_042634304.1 cysteine protease StiP family protein -
H1R14_RS00770 () 37599..38555 + 957 WP_000797366.1 HpcH/HpaI aldolase/citrate lyase family protein -
H1R14_RS00775 () 38566..39774 + 1209 WP_000506887.1 DUF4236 domain-containing protein -
H1R14_RS00780 () 39792..40259 + 468 WP_001176767.1 tellurium resistance protein TerW -
H1R14_RS00785 () 40722..41360 + 639 WP_001388628.1 VWA domain-containing protein -
H1R14_RS00790 () 41383..42024 + 642 WP_000116681.1 TerD family protein -
H1R14_RS00795 () 42024..42662 + 639 WP_001253658.1 VWA domain-containing protein -
H1R14_RS00800 () 42748..43788 + 1041 WP_001253653.1 VWA domain-containing protein -
H1R14_RS00805 () 43788..45425 + 1638 WP_000081622.1 protein phosphatase 2C domain-containing protein -
H1R14_RS00810 () 45451..46950 + 1500 WP_001284313.1 kinase -
H1R14_RS00815 () 47117..48199 - 1083 WP_001232449.1 hypothetical protein -
H1R14_RS00820 () 48560..48772 - 213 WP_001285422.1 hypothetical protein -
H1R14_RS00825 () 48943..50118 + 1176 WP_000795947.1 tyrosine-type recombinase/integrase -
H1R14_RS00830 () 50140..50484 - 345 WP_001805195.1 hypothetical protein -
H1R14_RS00835 () 50420..51325 - 906 WP_001198594.1 hypothetical protein -
H1R14_RS00840 () 51335..52297 - 963 WP_000119427.1 DNA replication protein -
H1R14_RS00845 () 52388..52771 - 384 WP_001424628.1 hypothetical protein -
H1R14_RS00850 () 52851..53387 - 537 WP_000107168.1 hypothetical protein -
H1R14_RS00855 () 53461..53718 - 258 WP_001371933.1 hypothetical protein -
H1R14_RS00860 () 53726..54316 - 591 WP_000578892.1 hypothetical protein -
H1R14_RS00865 () 54361..54636 - 276 WP_001004201.1 hypothetical protein -
H1R14_RS00870 () 54900..55856 - 957 WP_046788424.1 hypothetical protein -
H1R14_RS00875 () 56028..56966 - 939 WP_000472043.1 hypothetical protein -
H1R14_RS00880 () 57033..57953 - 921 WP_000991699.1 hypothetical protein -
H1R14_RS00885 () 58022..58957 - 936 WP_000691118.1 hypothetical protein -
H1R14_RS00890 () 59028..59906 - 879 WP_000762004.1 hypothetical protein -
H1R14_RS00895 () 59985..60506 - 522 WP_001010933.1 hypothetical protein -
H1R14_RS00900 () 60777..62009 - 1233 WP_000220381.1 toprim domain-containing protein -
H1R14_RS00905 () 62123..62344 - 222 WP_042634307.1 hypothetical protein -
H1R14_RS00910 () 62416..64428 - 2013 WP_104918329.1 VWA domain-containing protein -
H1R14_RS00915 () 64506..65633 - 1128 WP_000174395.1 DUF3150 domain-containing protein -
H1R14_RS00920 () 65697..65870 - 174 WP_001176647.1 hypothetical protein -
H1R14_RS00925 () 66002..67060 - 1059 WP_000063335.1 AAA family ATPase -
H1R14_RS00930 () 67118..68095 - 978 WP_042634278.1 hypothetical protein -
H1R14_RS00935 () 68156..68533 - 378 WP_000120822.1 hypothetical protein -
H1R14_RS00940 () 68591..69463 - 873 WP_000041927.1 hypothetical protein -
H1R14_RS00945 () 69858..71642 - 1785 WP_042634277.1 IncHI-type conjugal transfer helicase TrhI -
H1R14_RS00950 () 71822..72694 + 873 WP_000771832.1 hypothetical protein -
H1R14_RS00955 () 72723..75911 - 3189 WP_000776705.1 IncHI-type conjugal transfer protein TrhN -
H1R14_RS00960 () 75930..76985 - 1056 WP_181196458.1 IncHI-type conjugal transfer protein TrhU -
H1R14_RS00965 () 77130..78641 - 1512 WP_001256133.1 hypothetical protein -
H1R14_RS00970 () 78628..79140 - 513 WP_001286759.1 IncHI-type conjugal transfer protein TrhF -
H1R14_RS00975 () 79418..83722 - 4305 WP_001232107.1 IncHI-type conjugal transfer protein RSP -
H1R14_RS00980 () 83842..84396 - 555 WP_000235777.1 hypothetical protein -
H1R14_RS00985 () 84405..84830 - 426 WP_000836980.1 hypothetical protein -
H1R14_RS00990 () 84820..85272 - 453 WP_000952689.1 hypothetical protein -
H1R14_RS00995 () 85676..86314 - 639 WP_001107537.1 hypothetical protein -
H1R14_RS01000 () 86326..87363 - 1038 WP_000818955.1 hypothetical protein -
H1R14_RS01005 () 87727..88674 + 948 WP_046788422.1 metallophosphoesterase -
H1R14_RS01010 () 88671..89507 + 837 WP_042634275.1 hypothetical protein -
H1R14_RS01015 () 89500..90021 + 522 WP_000598516.1 hypothetical protein -
H1R14_RS01020 () 90018..90407 + 390 WP_001572421.1 hypothetical protein -
H1R14_RS01025 () 90469..91473 - 1005 WP_032192066.1 ParB N-terminal domain-containing protein -
H1R14_RS01030 () 91470..92723 - 1254 WP_032084678.1 hypothetical protein VF
H1R14_RS01035 () 96560..99241 - 2682 WP_000387412.1 TraC family protein -
H1R14_RS01040 () 99250..100200 - 951 WP_001022587.1 IncHI-type conjugal transfer lipoprotein TrhV -
H1R14_RS01045 () 100210..101025 - 816 WP_001639798.1 protein-disulfide isomerase HtdT -
H1R14_RS01050 () 101081..101503 - 423 WP_000251250.1 hypothetical protein -
H1R14_RS01055 () 101571..102926 - 1356 WP_000351841.1 IncHI-type conjugal transfer protein TrhB -
H1R14_RS01060 () 102916..103377 - 462 WP_000521240.1 hypothetical protein -
H1R14_RS01065 () 103379..104650 - 1272 WP_000592090.1 type-F conjugative transfer system secretin TraK -
H1R14_RS01070 () 104650..105438 - 789 WP_000783153.1 pilus assembly protein -
H1R14_RS01075 () 105450..105767 - 318 WP_000043357.1 hypothetical protein -
H1R14_RS01080 () 105818..106171 - 354 WP_000423602.1 hypothetical protein -
H1R14_RS01085 () 107320..108195 - 876 WP_000594612.1 RepB family plasmid replication initiator protein -
H1R14_RS01090 () 108672..109727 + 1056 WP_001065779.1 hypothetical protein -
H1R14_RS01095 () 109745..109945 + 201 WP_000594931.1 hypothetical protein -
H1R14_RS01100 () 109942..110658 + 717 WP_046788421.1 zinc finger-like domain-containing protein -
H1R14_RS01105 () 110893..111747 + 855 WP_000970403.1 hypothetical protein -
H1R14_RS01110 () 111756..112568 - 813 WP_001121575.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
H1R14_RS01115 () 112938..114101 + 1164 WP_000114859.1 hypothetical protein -
H1R14_RS01120 () 114349..116040 + 1692 WP_042634269.1 DUF4942 domain-containing protein -
H1R14_RS01125 () 116037..116213 + 177 WP_000070863.1 hypothetical protein -
H1R14_RS01130 () 116262..116723 + 462 WP_000502497.1 hypothetical protein -
H1R14_RS01135 () 116704..116985 + 282 WP_000535422.1 hypothetical protein -
H1R14_RS01140 () 117043..117639 - 597 WP_046788420.1 hypothetical protein -
H1R14_RS01150 () 119378..121834 + 2457 WP_001572417.1 DUF4165 domain-containing protein -
H1R14_RS01155 () 122942..124030 + 1089 WP_000159529.1 IncHI-type plasmid replication initiator protein RepHI2 -
H1R14_RS01160 () 126028..128448 - 2421 WP_000681217.1 DUF4165 domain-containing protein -
H1R14_RS01165 () 128913..129500 - 588 WP_000491822.1 hypothetical protein -
H1R14_RS01170 () 129545..129808 - 264 WP_000149861.1 hypothetical protein -
H1R14_RS01175 () 129902..130663 - 762 WP_001167416.1 methyltransferase -
H1R14_RS01180 () 130744..131508 - 765 WP_000277497.1 hypothetical protein -
H1R14_RS01185 () 131829..131996 + 168 WP_000803860.1 hypothetical protein -
H1R14_RS01190 () 132069..134459 + 2391 WP_000694550.1 Ig-like domain repeat protein -
H1R14_RS01195 () 134894..135364 + 471 WP_000170638.1 hypothetical protein -
H1R14_RS01200 () 135414..135710 + 297 WP_000581857.1 type II toxin-antitoxin system RelE/ParE family toxin -
H1R14_RS01205 () 135719..136900 + 1182 Protein_133 flavin reductase family protein -
H1R14_RS01210 () 137308..137685 + 378 WP_000853477.1 hypothetical protein -
H1R14_RS01215 () 137780..138208 + 429 WP_001133498.1 hypothetical protein -
H1R14_RS01220 () 138273..138530 + 258 WP_001290637.1 hypothetical protein -
H1R14_RS01225 () 138686..139294 + 609 WP_000833773.1 hypothetical protein -
H1R14_RS01230 () 139463..140713 + 1251 WP_000104323.1 site-specific DNA-methyltransferase -
H1R14_RS01235 () 140994..141140 + 147 WP_001426334.1 hypothetical protein -
H1R14_RS01240 () 141556..141882 + 327 WP_000556022.1 hypothetical protein -
H1R14_RS01245 () 141948..142205 + 258 WP_042634392.1 hypothetical protein -
H1R14_RS01250 () 142432..143223 + 792 WP_046788417.1 hypothetical protein -
H1R14_RS01255 () 143275..143724 + 450 WP_000182314.1 hypothetical protein -
H1R14_RS01260 () 143897..146281 + 2385 WP_000469466.1 phosphoadenosine phosphosulfate reductase family protein -
H1R14_RS01265 () 146531..146770 + 240 WP_001180999.1 hypothetical protein -
H1R14_RS01270 () 146833..147144 + 312 WP_000172889.1 hypothetical protein -
H1R14_RS01275 () 147141..147329 + 189 WP_000159618.1 hypothetical protein -
H1R14_RS01280 () 147390..147923 + 534 WP_000241454.1 HNH endonuclease -
H1R14_RS01285 () 148038..148646 + 609 WP_000280722.1 hypothetical protein -
H1R14_RS01290 () 148874..149101 + 228 WP_000276410.1 hypothetical protein -
H1R14_RS01295 () 149281..149601 + 321 WP_001133831.1 hypothetical protein -
H1R14_RS01300 () 150117..150428 + 312 WP_001165367.1 DUF957 domain-containing protein -
H1R14_RS01305 () 150433..150750 + 318 Protein_153 hypothetical protein -
H1R14_RS01310 () 150853..152025 + 1173 WP_058800785.1 IS21 family transposase IS21 Transposase
H1R14_RS01315 () 152025..152822 + 798 WP_000544830.1 IS21 family transposase IS21 Transposase
H1R14_RS01320 () 152871..153059 + 189 WP_159130763.1 hypothetical protein -
H1R14_RS01325 () 153612..153815 + 204 WP_000781545.1 hypothetical protein -
H1R14_RS01330 () 153870..154094 + 225 WP_000551490.1 hypothetical protein -
H1R14_RS01335 () 154134..154334 + 201 WP_000814954.1 hypothetical protein -
H1R14_RS01340 () 154557..154868 + 312 WP_001424621.1 hypothetical protein -
H1R14_RS01345 () 155243..155524 + 282 WP_000703843.1 hypothetical protein -
H1R14_RS01350 () 155740..156345 + 606 WP_000137793.1 hypothetical protein -
H1R14_RS01355 () 156678..158047 + 1370 WP_087522250.1 IS3 family transposase IS150 Transposase
H1R14_RS01360 () 158146..158304 + 159 WP_000783758.1 type I toxin-antitoxin system Hok family toxin -
H1R14_RS01365 () 158499..159611 - 1113 WP_001300563.1 IS4 family transposase IS421 Transposase
H1R14_RS01370 () 160073..160276 - 204 WP_001015182.1 hypothetical protein -
H1R14_RS01375 () 160322..160672 - 351 WP_000743060.1 hypothetical protein -
H1R14_RS01380 () 160732..161331 - 600 WP_001031421.1 hypothetical protein -
H1R14_RS01385 () 161431..162375 - 945 WP_000778030.1 DUF5417 domain-containing protein -
H1R14_RS01390 () 162885..163061 + 177 WP_001371930.1 hypothetical protein -
H1R14_RS01395 () 163477..164661 + 1185 WP_001272970.1 hypothetical protein -
H1R14_RS01400 () 164727..165008 + 282 WP_000710431.1 hypothetical protein -
H1R14_RS01405 () 165265..165471 + 207 WP_000893963.1 hypothetical protein -
H1R14_RS01410 () 165592..165888 + 297 WP_000744346.1 hypothetical protein -
H1R14_RS01415 () 165933..166370 + 438 WP_000286107.1 hypothetical protein -
H1R14_RS01420 () 166438..166974 + 537 WP_000800252.1 hypothetical protein -
H1R14_RS01425 () 167139..167507 + 369 WP_000537761.1 hypothetical protein -
H1R14_RS01430 () 167940..168242 + 303 WP_000442694.1 hypothetical protein -
H1R14_RS01435 () 168599..168883 + 285 WP_001032029.1 hypothetical protein -
H1R14_RS01440 () 168949..169302 + 354 WP_000210769.1 hypothetical protein -
H1R14_RS01445 () 169589..170320 - 732 WP_000338612.1 NERD domain-containing protein -
H1R14_RS01450 () 170322..171503 - 1182 WP_000952983.1 S49 family peptidase -
H1R14_RS01455 () 171514..172176 - 663 WP_000718549.1 DUF4400 domain-containing protein -
H1R14_RS01460 () 172163..173272 - 1110 WP_046788496.1 hypothetical protein -
H1R14_RS01465 () 173272..175356 - 2085 WP_046788497.1 conjugative transfer system coupling protein TraD -
H1R14_RS01470 () 175356..178502 - 3147 WP_001011151.1 hypothetical protein Relaxase
H1R14_RS01475 () 178512..179249 - 738 WP_000128631.1 hypothetical protein -
H1R14_RS01480 () 179246..179731 - 486 WP_000071885.1 hypothetical protein -
H1R14_RS01485 () 180490..181290 + 801 WP_000004209.1 hypothetical protein -
H1R14_RS01490 () 181292..181804 + 513 WP_001140953.1 hypothetical protein -
H1R14_RS01495 () 182398..183444 + 1047 WP_000196728.1 thioredoxin family protein -
H1R14_RS01500 () 183434..184849 + 1416 WP_001257292.1 IncHI-type conjugal transfer protein TrhH -
H1R14_RS01505 () 184858..188811 + 3954 WP_046788498.1 conjugal transfer protein TraG N-terminal domain-containing protein -
H1R14_RS01510 () 188992..190281 + 1290 WP_001284752.1 HNH endonuclease -
H1R14_RS01515 () 190389..190907 - 519 WP_015059500.1 hypothetical protein -
H1R14_RS01520 () 191038..191577 - 540 WP_000494967.1 hypothetical protein VF
H1R14_RS01525 () 191725..192474 + 750 WP_000720274.1 thioredoxin fold domain-containing protein -
H1R14_RS01530 () 192499..192891 + 393 WP_000843244.1 hypothetical protein -
H1R14_RS01535 () 192925..193347 + 423 WP_000025803.1 hypothetical protein -
H1R14_RS01540 () 193407..194018 - 612 WP_000620990.1 hypothetical protein -
H1R14_RS01545 () 194125..194934 + 810 WP_000031377.1 DsbA family protein -
H1R14_RS01550 () 194980..196239 - 1260 WP_042634445.1 translesion error-prone DNA polymerase V subunit UmuC -
H1R14_RS01555 () 196223..196657 - 435 WP_000111290.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
H1R14_RS01560 () 196851..197468 + 618 WP_001130842.1 hypothetical protein -
H1R14_RS01565 () 197618..197974 + 357 WP_000770127.1 hypothetical protein -
H1R14_RS01570 () 198225..198395 + 171 WP_152919720.1 hypothetical protein -
H1R14_RS01575 () 198432..199136 + 705 WP_001067858.1 IS6 family transposase IS15DI Transposase
H1R14_RS01580 () 199471..199863 + 393 WP_089079287.1 pyridoxamine 5'-phosphate oxidase family protein -
H1R14_RS01585 () 200183..200569 - 387 WP_063102497.1 bleomycin binding protein -
H1R14_RS01590 () 200763..201466 - 704 Protein_210 IS6 family transposase -
H1R14_RS01595 () 201534..202832 + 1299 Protein_211 Tn3 family transposase TnpA_Tn3 Transposase
H1R14_RS01600 () 202857..203021 - 165 WP_072644484.1 cupin domain-containing protein -
H1R14_RS01605 () 203002..203208 + 207 Protein_213 QacE family quaternary ammonium compound efflux SMR transporter -
H1R14_RS01610 () 203202..203687 + 486 Protein_214 dihydropteroate synthase -
H1R14_RS01615 () 203742..204446 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
H1R14_RS01620 () 204507..205436 + 930 Protein_216 CmlA/FloR family chloramphenicol efflux MFS transporter -
H1R14_RS01625 () 205464..205769 + 306 WP_001255015.1 HTH-type transcriptional regulator HdfR VF
H1R14_RS01630 () 205881..206042 + 162 Protein_218 IS91 family transposase -
H1R14_RS01635 () 206131..207087 + 957 WP_063609966.1 IS21 family transposase ISEc57 Transposase
H1R14_RS01640 () 207124..207828 + 705 WP_001067858.1 IS6 family transposase IS15DI Transposase
H1R14_RS01645 () 208112..208768 + 657 WP_012537714.1 hypothetical protein ARG
H1R14_RS01650 () 209052..209711 + 660 WP_077898834.1 SprT-like domain-containing protein -
H1R14_RS01655 () 209657..210361 - 705 WP_001067855.1 IS6 family transposase IS26 Transposase
H1R14_RS01660 () 210426..211106 - 681 Protein_224 IS91 family transposase ISAzo26 Transposase
H1R14_RS01665 () 211398..211952 + 555 WP_063840321.1 hypothetical protein ARG
H1R14_RS01670 () 212083..212913 + 831 WP_001334766.1 hypothetical protein ARG
H1R14_RS01675 () 213051..213683 + 633 WP_000186237.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase VF
H1R14_RS01680 () 213768..214220 + 453 WP_001749986.1 hypothetical protein ARG
H1R14_RS01685 () 214443..214790 + 348 WP_000679427.1 Spermidine export protein MdtJ ARG
H1R14_RS01690 () 214784..215623 + 840 WP_000259031.1 hypothetical protein ARG
H1R14_RS01695 () 215751..215954 + 204 WP_000376616.1 hypothetical protein -
H1R14_RS01700 () 216072..216776 - 705 WP_001067855.1 IS6 family transposase IS26 Transposase

Similar MGE(s)


Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.

Detail Organism MGE type Related TA Genome accession Coordinates Mash
distance