Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0071	Z4049 (RpoS)	T6SS00006	protein	Global regulator	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	3654380..3655372 (-)	446003635	Z1921 (KatN) (↓)
REG0190	ypk_3425 (RpoS)	T6SS00260	protein	Global regulator	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	3759239..3760237 (-)	499505140	T6SS-4 promoter region (↓)
REG0206	ETAE_2873 (RpoS)	T6SS00372	protein	TCS regulatory pathway	Edwardsiella tarda EIB202	chromosome (NC_013508)	3025094..3026080 (-)	502612732	ETAE_0886 (esrB) promoter (↑)
REG0346	BN49_RS06445 (rpoS)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	1060243..1061235 (+)	489004494	TssB transcription (↓)
REG0364	Y113_RS11250 (RpoS)	T6SS00791	protein	Transcription regulator	Edwardsiella tarda PPD130/91	chromosome (NZ_JACQ01000026)	9122..10108 (-)	502612732	EvpQ transcription and protein level (↑)
REG0018	PA4462 (RpoN)	T6SS00003 ; T6SS00004 ; T6SS00005	protein	Global regulator	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4992870..4994363 (+)	15599658	PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0069	PA4462 (rpoN)	T6SS00004	protein	Global regulator	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4992870..4994363 (+)	15599658	T6SS transcript (↑)
REG0075	PP_0952 (RopN)	T6SS00014	protein	c-di-GMP regulatory pathway	Pseudomonas putida KT2440	chromosome (NC_002947)	1094567..1096060 (-)	26987688	PP_4373 (FleQ) (↑)
REG0095	ECA0288 (rpoN)	T6SS00040	protein	Global regulator	Pectobacterium atrosepticum SCRI1043	chromosome (NC_004547)	336007..337440 (-)	499404454	T6SS gene cluster promoter (PECA3445) (↓); Hcp gene cluster promoter (PECA4275) (↓); Hcp gene cluster promoter (PECA2866) (↓)
REG0124	VFFQA001_02105 (rpoN)	T6SS01277	protein	Global regulator	Aliivibrio fischeri strain FQ-A001	chromosome (NZ_SJSX01000002)	62995..64464 (-)	501526930	hcp1/hcp transcript (↓)
REG0182	VC0395_A2109 (rpoN)	T6SS00208	protein	Global regulator	Vibrio cholerae O395	chromosome (NC_009457)	2265078..2266541 (+)	446727517	T6SS gene cluster promoter (PVCA0107/VC0395_RS00160) (↓); hcp promoter (PVCA0017/VC0395_0116) (↓)
REG0184	Mmwyl1_2418 (RpoN)	T6SS00216	protein	Global regulator	Marinomonas sp. MWYL1	chromosome (NC_009654)	2725212..2726687 (+)	501017436	T6SS gene cluster promoter (PMWYL1196/PMWYL1195) (↓)
REG0300	VCA1552_RS11610 (RpoN)	T6SS01112	protein	Global regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2488023..2489486 (+)	446727517	Hcp expression and secretion (↓)
REG0317	VCV52_RS13040 (rpoN)	T6SS00790	protein	Global regulator	Vibrio cholerae V52	chromosome (NZ_KQ410502)	216932..218395 (-)	446727517	Unknown (↓)
REG0345	BN49_RS03755 (rpoN)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	533564..534997 (-)	489007850	TssB transcription (↓)
REG0354	AHA_3923 (rpoN)	T6SS00157	protein	Global regulator	Aeromonas hydrophila subsp. hydrophila ATCC 7966	chromosome (NC_008570)	4350676..4352115 (-)	117618923	T6SS gene cluster promoter (PAHA1826) (↓)
REG0249	EC042_RS18855 (FIS)	T6SS00464	protein	Transcription regulator	Escherichia coli 042	chromosome (NC_017626)	3772795..3773091 (+)	446385050	EC042_RS26480 (aggR) promoter (↓)
REG0011	Y880_05180 (rmsF)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	RsmA regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	5147809..5148024 (-)	489186811	tssA1 expression (↑)
REG0041	PA5183a (RsmN)	T6SS00003	protein	RsmA regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	5836470..5836685 (-)	554753081	FHA (PA0081) (↑); PA3487 (pldA) expression (↑)
REG0102	VP_RS12395 (CsrA)	T6SS00043 ; T6SS00044	protein	carbon storage regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2688313..2688510 (-)	490550575	Hcp and vgrG expression and secretion (↓)
REG0158	PA14_52570 (RsmA)	T6SS00150	protein	RsmA regulatory pathway	Pseudomonas aeruginosa UCBPP-PA14	chromosome (NC_008463)	4660393..4660578 (-)	489176459	RSMF (↑); tssA1 RNA (↑); RSMA (↑)
REG0193	YPK_3372 (CsrA)	T6SS00260	protein	carbon storage regulatory pathway	Yersinia pseudotuberculosis YPIII	chromosome (NC_010465)	3698608..3698793 (-)	169752121	YPK_3567 (RovC) (↑); YPK_3563 transcript (↑)
REG0019	PC1_3721 (Hfq)	T6SS01001	protein	QS regulatory pathway	Pectobacterium carotovorum subsp. carotovorum PC1	chromosome (CP001657)	4199890..4200189 (-)	251756660	hcp expression (↓)
REG0299	VCA1552_RS00505 (hfq)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	92673..92936 (+)	446974616	VCA1552_00351 (HapR) (↑)
REG0344	BN49_RS26620 (hfq)	T6SS01214	protein	QS regulatory pathway	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	5173711..5174019 (-)	488974767	TssB transcription (↓)
REG0367	H78_RS02920 (Hfq)	-	protein	QS regulatory pathway	Pseudomonas protegens strain H78	chromosome (NZ_CP013184)	662937..663197 (+)	495196588	T6SS transcription level (↓)
REG0302	VCA1552_RS12925 (cya)	T6SS01112	protein	QS regulatory pathway; cAMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2747346..2749877 (-)	447072406	VCA1552_RS12025 (crp) (↓)
REG0298	VCA1552_RS01665 (LuxS)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	319588..320106 (+)	447052971	Hcp expression and secretion (↓)
REG0312	VCA1552_RS12850 (cdgJ)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2733319..2734545 (-)	446210986	the levels of c-di-GMP (↑)
REG0100	VP_RS12495 (barA)	T6SS00043 ; T6SS00044	protein	TCS-HK	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2712492..2715290 (+)	491625032	Hcp expression and secretion (↓)
REG0150	Psyr_3698 (gacS)	T6SS00086	protein	TCS-HK	Pseudomonas syringae pv. syringae B728a	chromosome (NC_007005)	4385550..4388303 (+)	499587812	T6SS transcription (↓)
REG0330	Pchl3084_4333 (gacS)	T6SS01266 ; T6SS01267	protein	TCS-RR	Pseudomonas chlororaphis subsp. aureofaciens 30-84	chromosome (NZ_CM001559)	5113748..5116501 (+)	496335944	Pchl3084_3491 (gacA) (↓)
REG0351	PSEBR_RS22825 (GacS)	T6SS00431 ; T6SS00432	protein	TCS-RR	Pseudomonas brassicacearum subsp. brassicacearum NFM421	chromosome (NC_015379)	5188012..5190762 (+)	503459407	hcp1 transcript (↓); hcp2 transcript (↓)
REG0012	Y880_02189 (gacA/uvrY)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	TCS-RR	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2199690..2200334 (-)	510933056	hcp expression (↓)
REG0044	PA2586 (gacA)	T6SS00003	protein	TCS-RR	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	2925788..2926432 (-)	15597782	hcp1 (PA0085) expression and secretion (↓)
REG0091	PFL_RS17995 (gacA/uvrY/SirA)	T6SS00035	protein	TCS-RR	Pseudomonas protegens Pf-5	chromosome (NC_004129)	4110513..4111154 (-)	499374263	T6SS transcripts (↓)
REG0101	VP_RS09470 (UvrY)	T6SS00043 ; T6SS00044	protein	TCS-RR	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2037468..2038112 (-)	491529158	CsrB (↓)
REG0253	PAM18_RS12360 (gacA)	T6SS00612 ; T6SS00613 ; T6SS00611	protein	TCS-RR	Pseudomonas aeruginosa M18	chromosome (NC_017548)	2625815..2626459 (+)	489180874	hsiA1 operon transcript (↓); hsiA2 operon transcript (↓); hsiA3 operon transcript (↓)
REG0331	Pchl3084_3491 (gacA)	T6SS01266 ; T6SS01267	protein	TCS-HK	Pseudomonas chlororaphis subsp. aureofaciens 30-84	chromosome (NZ_CM001559)	4134794..4135435 (-)	496335231	Pchl3084_5419 (rsmY)promotor (↓); Pchl3084_1190 (rsmZ)promotor (↓); Pchl3084_1189 (rpoS)transcript (↓)
REG0296	VCA1552_RS03895 (LuxO)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	818142..819509 (+)	487814784	Hcp expression and secretion (↑)
REG0023	GPY04_RS00870 (HapR)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	164935..165546 (-)	446262243	Unknown (↓)
REG0112	VP2516 (opaR)	T6SS00043 ; T6SS00044	protein	QS regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2653215..2653829 (+)	28899290	vp1407 (↑); VP1388 promotor (↑); VP1400 promotor (↑); VP1409 promotor (↑); T6SS2 (Hcp2) expression and secretion (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1043-1028 transcript (↓); VPA1044-1046 transcript (↓)
REG0118	VP2516 (OpaR)	T6SS00043	protein	Global regulator	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2653215..2653829 (+)	28899290	T6SS1 (↓)
REG0122	VF2177 (LitR)	T6SS00084	protein	QS regulatory pathway	Aliivibrio fischeri ES114	chromosome (NC_006840)	2446537..2447142 (+)	59712784	Unknown (↓)
REG0275	V12G01_21538 (LuxR)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000053)	13551..14165 (-)	91187814	T6SS2 transcripts (↓); V12G01-18672 (vqsA) promotor
REG0293	VCA1552_00351 (HapR)	T6SS01112	protein	Global regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	340447..341058 (-)	446262243	Unknown; Hcp expression and secretion
REG0355	N779_15250 (VtpR)	T6SS01280 ; T6SS01281	protein	QS regulatory pathway	Vibrio coralliilyticus OCN008	chromosome (AVOO01000045)	7579..8184 (-)	536747482	Unknown (↓)
REG0301	VCA1552_RS12025 (crp)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2558371..2559003 (+)	446164894	Hcp expression and secretion (↓)
REG0297	VCA1552_RS17020 (cqsa)	T6SS01112	protein	QS regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024868)	455238..456407 (-)	446962656	Hcp expression and secretion (↓)
REG0111	VP2762 (AphA)	T6SS00043 ; T6SS00044	protein	QS regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2929909..2930448 (+)	28899536	T6SS1 transcript (↑); T6SS2 (VPA1027-1024, VPA1043-1028,VPA1044-1046) transcripts (↑)
REG0287	V12G01_14830 (aphA)	T6SS00939	protein	QS regulatory pathway	Vibrio alginolyticus 12G01	chromosome (AAPS01000027)	42305..42880 (+)	91188695	V12G01-18672 (vqsA) promotor
REG0114	VP0820 (ToxR)	T6SS00043	protein	Transcription regulator	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	847892..848770 (-)	28897594	VP1400 promotor (↑); VP1409 promotor (↑)
REG0074	PP_4373 (FleQ)	T6SS00014	protein	c-di-GMP regulatory pathway	Pseudomonas putida KT2440	chromosome (NC_002947)	4963882..4965357 (-)	26991062	K1-T6SS (hcp1) expression (↑)
REG0309	VCA1552_RS04310 (cdgK)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	901594..903063 (+)	487807420	the levels of c-di-GMP (↓)
REG0310	VCA1552_RS04130 (cdgH)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	861187..863208 (+)	446848493	the levels of c-di-GMP (↓)
REG0308	VCA1552_RS10420 (cdgL)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2215844..2217361 (-)	446975354	the levels of c-di-GMP (↓)
REG0291	VCA1552_02536 (CdpA/YfgF)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2739669..2742158 (-)	446417730	the levels of c-di-GMP (↓)
REG0311	VCA1552_RS02130 (rocS)	T6SS01112	protein	c-di-GMP regulatory pathway	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	425233..427287 (-)	447034155	the levels of c-di-GMP (↑)
REG0116	VPA1088 (cdpA)	T6SS00043	protein	c-di-GMP regulatory pathway	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004605)	1147728..1150091 (+)	28900943	VP1028 (TfoY) (↑)