T4SS ID	43
Strain	Legionella pneumophila 2300/99 Alcoy
Replicon	chromosome [Browse all T4SS(s) in this replicon]
Accession	NC_014125
Location	526476..562636; 649247..649873; 3132162..3150833
Name	Dot/Icm
Function	effector translocation
Classification	Type IVB; Type I
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	DotA	DotB	DotC	DotD	IcmB	IcmC	IcmD	IcmE	IcmF
Number	1	1	1	1	1	2	1	1	1
Component	IcmG	IcmH	IcmJ	IcmK	IcmL	IcmM	IcmN	IcmO	IcmP
Number	1	1	1	1	1	1	1	1	1
Component	IcmQ	IcmR	IcmS	IcmT	IcmV	IcmW	IcmX	LvgA
Number	1	1	1	0	1	1	1	1

The information of T4SS components from NC_014125
Region 1: 526476..562636
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	lpa_00674	521872..523872 [+], 2001	296105988	hypothetical protein
2	lpa_00676	524298..524450 [-], 153	296105989	hypothetical protein
3	lpa_00678	524691..525467 [-], 777	296105990	hypothetical protein
4	lpa_00680	525833..526045 [+], 213	296105991	hypothetical protein
5	lpa_00681	526187..526378 [-], 192	296105992	hypothetical protein
6	lpa_00683	526476..526820 [+], 345	296105993	protein IcmS	IcmS
7	lpa_00684	526920..527282 [+], 363	296105994	protein IcmR	IcmR
8	lpa_00685	527373..527948 [+], 576	296105995	protein IcmQ	IcmQ
9	lpa_00686	528135..529265 [+], 1131	296105996	protein IcmP (DotM)	IcmP
10	lpa_00687	529262..531613 [+], 2352	296105997	protein IcmO (DotL)	IcmO
11	lpa_00688 (lphA)	532017..532586 [+], 570	296105998	LphA (DotK)	IcmN
12	lpa_00689	532598..532882 [+], 285	296105999	protein IcmM (DotJ)	IcmM
13	lpa_00690	532898..533536 [+], 639	296106000	protein IcmL (DotI)	IcmL
14	lpa_00691	533539..534621 [+], 1083	296106001	protein IcmK (DotH)	IcmK
15	lpa_00694	534626..537772 [+], 3147	296106002	protein IcmE (DotG)	IcmE
16	lpa_00697	537787..538596 [+], 810	296106003	protein IcmG (DotF)	IcmG
17	lpa_00698 (icmC)	538604..539188 [+], 585	296106004	hypothetical protein	IcmC
18	lpa_00699 (icmD)	539214..539612 [+], 399	296106005	protein IcmD (DotP)	IcmD
19	lpa_00700	539812..540438 [+], 627	296106006	protein IcmJ (DotN)	IcmJ
20	lpa_00701	540474..543503 [+], 3030	296106007	protein IcmB (DotO)	IcmB
21	lpa_00703 (tphA)	543649..544905 [-], 1257	296106008	proline/betaine transport protein like protein
22	lpa_00704 (icmF)	544908..547829 [-], 2922	296106009	hypothetical protein	IcmF
23	lpa_00705	547829..548614 [-], 786	296106010	hypothetical protein	IcmH
24	lpa_00707 (purH)	548899..550488 [-], 1590	296106011	phosphoribosylaminoimidazolecarboxamide formyltransferase
25	lpa_00708 (prmA)	550515..551384 [-], 870	296106012	50S ribosomal protein L11 methyltransferase
26	lpa_00709 (accC)	551386..552729 [-], 1344	296106013	acetyl-CoA carboxylase, biotin carboxylase subunit
27	lpa_00710 (accB)	552742..553224 [-], 483	296106014	acetyl-CoA carboxylase biotin carboxyl carrier protein
28	lpa_00711 (aroQ)	553237..553920 [-], 684	296106015	3-dehydroquinate dehydratase
29	lpa_00712	553876..555666 [+], 1791	296106016	oxaloacetate decarboxylase, alpha subunit
30	lpa_00713 (lasB)	556232..557863 [+], 1632	296106017	zinc metalloprotease
31	lpa_00714 (mhpC)	557865..558713 [-], 849	296106018	hydrolases or acyltransferases (alpha/beta hydrolase superfamily)
32	lpa_00716	559182..559937 [+], 756	296106019	endonuclease/exonuclease/phosphatase family protein
33	lpa_00720	560116..561126 [-], 1011	296106020	fructose-bisphosphate aldolase, class I
34	lpa_00722 (poxF)	561276..561968 [-], 693	296106021	phenol hydroxylase
35	lpa_00724	562094..562636 [+], 543	296106022	protein IcmC (DotV)-like protein	IcmC
36	lpa_00726	562838..563122 [-], 285	296106023	hypothetical protein
37	lpa_00729	563352..564089 [+], 738	296106024	CDP-diacylglycerol--serine O-phosphatidyltransferase
38	lpa_00730 (ptsH)	564331..564600 [-], 270	296106025	sugar transport PTS system phosphocarrier HPr protein
39	lpa_00731	564791..565090 [-], 300	296106026	putative sigma-54 modulation protein
40	lpa_00732 (rpoN)	565117..566511 [-], 1395	296106027	RNA polymerase sigma-54 factor
41	lpa_00733 (rpmG)	566723..566887 [-], 165	296106028	50S ribosomal protein L33
42	lpa_00734 (rpmB)	566902..567102 [-], 201	296106029	50S ribosomal protein L28
43	lpa_00736	567236..567397 [+], 162	296106030	hypothetical protein
Region 2: 649247..649873
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	lpa_00836	645585..646073 [-], 489	296106112	hypothetical protein
2	lpa_00837	646147..647349 [+], 1203	296106113	hypothetical protein
3	lpa_00838	647352..647696 [+], 345	296106114	hypothetical protein
4	lpa_00839	647777..647974 [+], 198	296106115	hypothetical protein
5	lpa_00840	648037..648759 [-], 723	296106116	hypothetical protein
6	lpa_00841	649247..649873 [+], 627	296106117	virulence protein	LvgA
7	lpa_00842	649903..650280 [-], 378	296106118	hypothetical protein
8	lpa_00843	650430..651569 [-], 1140	296106119	signal transduction protein containing EAL and modified HD-GYP domains
9	lpa_00845	651890..652264 [+], 375	296106120	succinate dehydrogenase cytochrome b-556 subunit
10	lpa_00846	652258..652605 [+], 348	296106121	succinate dehydrogenase hydrophobic membrane anchor protein
11	lpa_00848 (sdhA)	652607..654376 [+], 1770	296106122	succinate dehydrogenase flavoprotein subunit
Region 3: 3132162..3150833
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	lpa_03902 (ftsY)	3127765..3128832 [-], 1068	296108316	signal recognition particle receptor
2	lpa_03903	3128895..3130220 [+], 1326	296108317	zinc protease (peptidase, M16 family)
3	lpa_03904	3130217..3131521 [+], 1305	296108318	zinc protease (peptidase, M16 family)
4	lpa_03905	3131518..3132063 [+], 546	296108319	putative methylase
5	lpa_03907	3132162..3132629 [+], 468	296108320	DotD	DotD
6	lpa_03908	3132610..3133521 [+], 912	296108321	DotC	DotC
7	lpa_03909	3133521..3134654 [+], 1134	296108322	DotB	DotB
8	lpa_03910 (ushA)	3134736..3136463 [+], 1728	296108323	5'-nucleotidase
9	lpa_03911	3136505..3137302 [-], 798	296108324	hypothetical protein
10	lpa_03912	3137332..3138276 [-], 945	296108325	D-isomer specific 2-hydroxyacid dehydrogenase
11	lpa_03913	3138513..3139535 [+], 1023	296108326	UDP-N-acetylmuramyl tripeptide synthase
12	lpa_03915	3139809..3140696 [+], 888	296108327	4-hydroxy-2-ketovalerate aldolase
13	lpa_03916	3140835..3141542 [+], 708	296108328	hypothetical protein
14	lpa_03918 (leuB)	3141529..3143373 [+], 1845	296108329	Isocitrate/isopropylmalate dehydrogenase
15	lpa_03919	3143376..3144236 [+], 861	296108330	hypothetical protein
16	lpa_03920 (sbpA)	3144498..3145214 [-], 717	296108331	dienelactone hydrolase family protein
17	lpa_03922	3145345..3148404 [-], 3060	296108332	DotA	DotA
18	lpa_03923	3148401..3148775 [-], 375	296108333	protein IcmV	IcmV
19	lpa_03924	3148969..3149424 [+], 456	296108334	protein IcmW	IcmW
20	lpa_03925	3149424..3150833 [+], 1410	296108335	icmX Intracellular multiplication protein IcmX	IcmX
21	lpa_03927	3150837..3152405 [-], 1569	296108336	hypothetical protein
22	lpa_03928	3152832..3155375 [+], 2544	296108337	cation transporting ATPase PacS

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

Effectors

RavA, CegC1, lpa_00030, RavB, AnkQ/legA10, lpa_00056, lpa_00060, lpa_00062, Ceg2, Lem1, Ceg4, VipF, CegC2, lpa_00194, SdhB, RavD, RavC, lpa_00339, Ceg7, SidE/laiD, Ceg8, Ceg9, lpa_00450, lpa_00456, SdbA, LegG2, Ceg10, Lem2, lpa_00508, lpa_00578, lpa_00580, lpa_00596, SdhA, VipA, LegA7/ceg11, AnkG/ankZ/legA7, lpa_00633, LegY, AnkJ/legA11, Ceg14, Ceg15, AnkC/legA12, LegD2, lpa_00781, SidA, lpa_00996, WipB, AnkN/ankX legA8, Lem3, lpa_01108, RavH, Ceg18, RavI, LidA, RavJ, LegL1, lpa_01453, lpa_01459, SidK, RavK, Lem4, lpa_01719, RavL, RavM, Lem5, RavN, Ceg19, lpa_01748, lpa_01776, CegC3, Lem7, lpa_01789, lpa_01790, RavP, RavQ, lpa_01802, RavR, lpa_01826, RavS, VpdB, lpa_01901, LegT, VpdC, lpa_02119, LegK1, lpa_02162, Lgt3/legc5, RavX, Lem10, RavY, lpa_02292, LegC6, Lem11, LegL2, Ceg23, Lem23, lpa_02367, SidB, lpa_02390, LegL3, lpa_02399, lpa_02408, lpa_02409, lpa_02413, RavZ, lpa_02432, lpa_02433, MavA, lpa_02439, lpa_02442, PpeA/legC3, PpeB, lpa_02474, AnkI/legAS4, lpa_02538, lpa_02539, lpa_02570, RvfA, MarB, lpa_02606, Ceg25, Lem14, YlfB/legC2, lpa_02723, LegLC8, lpa_02762, lpa_02783, Lem15, Lem16, Lem17, RalF, LegC4, lpa_02857, LirA, LirB, PieA/lirC, PieC/lirE, PieD/lirF, PieE, PieF, PieG/legG1, SetA, lpa_02898, lpa_02992, Ceg3, LaiA/SdeA, LegK2, AnkB/legAU13/ceg27, MavC, lpa_03077, lpa_03078, SdeC, SdeC, SidJ, SdeB, lpa_03100, Lem19, LegS2, CegC4, CegC4, WipC, LegA2, Lem20, LpnE, lpa_03196, Lem21, lpa_03268, YlfA/legC7, AnkH/legA3, ankW, Ceg28, AnkK/legA5, lpa_03335, Lem22, MavE, MavF, lpa_03376, lpa_03446, SdbC, LegL6, Lem23, lpa_03507, Ceg29, VpdA, Lem24, LegA1, lpa_03529, Lem25, MavG, MavH, Ceg30, lpa_03550, lpa_03562, MavI, AnkF/legA14/ceg31, AnkD/legA15, lpa_03589, SdbB, LepB, MavJ, SidI/ceg32, lpa_03659, SdjA, SdeD, SdcA, SidC, MavL, LnaB, Lem27, lpa_03706, lpa_03707, lpa_03710, lpa_03727, lpa_03738, lpa_03743, LegK3, MavM, SidF, LegS1, Ceg33, Lem28, lpa_03846, lpa_03859, MavV, lpa_03929, LegD1, WipA, LegN, lpa_04004, lpa_04005, LepA, Lem29, MavN, Ceg34, lpa_04109, lpa_04110, lpa_04133, lpa_04176, lpa_04186, lpa_04193, lpa_04199, lpa_04255, lpa_04293, lpa_04300, lpa_04358, LegP, lpa_04397

The information of protein effectors
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	*
1	lpa_00011	11156..12415 [-], 1260	296105506	hypothetical protein	RavA
2	lpa_00016	14876..16453 [-], 1578	296105510	hypothetical protein	CegC1
3	lpa_00030	26673..27152 [+], 480	296105519	alpha helix protein	lpa_00030
4	lpa_00040	37635..38525 [-], 891	296105528	hypothetical protein	RavB
5	lpa_00049	44395..45912 [-], 1518	296105535	ankyrin repeat-containing protein	AnkQ/legA10
6	lpa_00056	47908..50094 [+], 2187	296105538	hypothetical protein	lpa_00056
7	lpa_00060	52598..52810 [-], 213	296105542	hypothetical protein	lpa_00060
8	lpa_00062	53020..53418 [+], 399	296105543	hypothetical protein	lpa_00062
9	lpa_00085	67156..67980 [+], 825	296105561	hypothetical protein	Ceg2
10	lpa_00132	107524..111498 [+], 3975	296105600	hypothetical protein	Lem1
11	lpa_00145	117968..119161 [+], 1194	296105606	hypothetical protein	Ceg4
12	lpa_00152	126406..127266 [-], 861	296105613	N-terminal acetyltransferase, GNAT family	VipF
13	lpa_00185	151787..155098 [+], 3312	296105635	ninein	CegC2
14	lpa_00194	159964..162432 [+], 2469	296105640	oxidoreductase	lpa_00194
15	lpa_00204 (sdhB)	165851..171448 [-], 5598	296105645	SdhB protein	SdhB
16	lpa_00322	248634..249611 [+], 978	296105728	hypothetical protein	RavD
17	lpa_00335	256035..256790 [-], 756	296105737	ABC transport system permease	RavC
18	lpa_00339	257456..258154 [+], 699	296105738	hypothetical protein	lpa_00339
19	lpa_00412	312077..313183 [+], 1107	296105785	hypothetical protein	Ceg7
20	lpa_00419 (sidE)	322273..326763 [-], 4491	296105791	SidE protein	SidE/laiD
21	lpa_00428	333716..334483 [+], 768	296105798	DNA repair protein	Ceg8
22	lpa_00436	342761..343486 [+], 726	296105805	hypothetical protein	Ceg9
23	lpa_00450	353167..354159 [+], 993	296105816	multidrug resistance protein A	lpa_00450
24	lpa_00456	358267..358665 [+], 399	296105820	hypothetical protein	lpa_00456
25	lpa_00477 (sdbA)	372650..376000 [+], 3351	296105835	SdbA protein, putative substrate of the Dot/Icm system	SdbA
26	lpa_00479	376080..377537 [+], 1458	296105836	Ras GEF	LegG2
27	lpa_00490	387358..388482 [-], 1125	296105844	hypothetical protein	Ceg10
28	lpa_00492	388739..389401 [+], 663	296105845	hypothetical protein	Lem2
29	lpa_00508	400048..400740 [+], 693	296105856	hypothetical protein	lpa_00508
30	lpa_00578	453269..453574 [-], 306	296105912	hypothetical protein	lpa_00578
31	lpa_00580	453760..456450 [-], 2691	296105913	hypothetical protein	lpa_00580
32	lpa_00596	463292..463891 [+], 600	296105925	RNA-binding protein containing a C-terminal EMAP domain	lpa_00596
33	lpa_00597	463931..468220 [-], 4290	296105926	SdhA, GRIP coiled-coil protein GCC185	SdhA
34	lpa_00613	481104..482162 [-], 1059	296105940	VipA	VipA
35	lpa_00629	489372..490037 [-], 666	296105952	hypothetical protein	LegA7/ceg11
36	lpa_00630 (arp)	490367..491818 [+], 1452	296105953	Ankyrin repeat protein	AnkG/ankZ/legA7
37	lpa_00633	493614..494138 [+], 525	296105955	hypothetical protein	lpa_00633
38	lpa_00657	509502..510785 [-], 1284	296105973	glucoamylase	LegY
39	lpa_00673	520801..521601 [-], 801	296105987	ankyrin repeat-containing protein	AnkJ/legA11
40	lpa_00674	521872..523872 [+], 2001	296105988	hypothetical protein	Ceg14
41	lpa_00678	524691..525467 [-], 777	296105990	hypothetical protein	Ceg15
42	lpa_00739	569298..570785 [+], 1488	296106033	ankyrin repeat-containing protein	AnkC/legA12
43	lpa_00776	601990..602922 [+], 933	296106066	phytanoyl-CoA dioxygenase	LegD2
44	lpa_00781	605029..605877 [-], 849	296106069	hypothetical protein	lpa_00781
45	lpa_00975	742189..743613 [-], 1425	296106209	SidA	SidA
46	lpa_00996	758656..759993 [-], 1338	296106222	hypothetical protein	lpa_00996
47	lpa_01005	768651..770225 [+], 1575	296106230	hypothetical protein	WipB
48	lpa_01082 (arp)	851943..854780 [+], 2838	296106283	Ankyrin repeat protein	AnkN/ankX legA8
49	lpa_01084	854787..856445 [-], 1659	296106284	hypothetical protein	Lem3
50	lpa_01108	878086..879081 [+], 996	296106304	hypothetical protein	lpa_01108
51	lpa_01135	896524..897948 [+], 1425	296106321	hypothetical protein	RavH
52	lpa_01360	1067557..1067826 [-], 270	296106478	hypothetical protein	Ceg18
53	lpa_01397	1095808..1096815 [-], 1008	296106509	hypothetical protein	RavI
54	lpa_01415	1111715..1113904 [-], 2190	296106523	LidA	LidA
55	lpa_01421	1119157..1120332 [-], 1176	296106527	hypothetical protein	RavJ
56	lpa_01423	1120494..1121381 [-], 888	296106528	Gala protein type 1, 3 or 4	LegL1
57	lpa_01453	1149673..1150914 [-], 1242	296106548	hypothetical protein	lpa_01453
58	lpa_01459	1155714..1156196 [-], 483	296106552	hypothetical protein	lpa_01459
59	lpa_01460	1156676..1158370 [-], 1695	296106553	hypothetical protein	SidK
60	lpa_01461	1158838..1159578 [+], 741	296106554	hypothetical protein	RavK
61	lpa_01709	1329993..1330394 [+], 402	296106725	hypothetical protein	Lem4
62	lpa_01719	1334973..1336235 [-], 1263	296106730	hypothetical protein	lpa_01719
63	lpa_01724	1338665..1339546 [-], 882	296106733	putative lipase	RavL
64	lpa_01725	1339787..1341658 [-], 1872	296106734	hypothetical protein	RavM
65	lpa_01728	1342406..1343101 [+], 696	296106736	hypothetical protein	Lem5
66	lpa_01730	1343195..1343833 [-], 639	296106737	hypothetical protein	RavN
67	lpa_01743	1355525..1356259 [+], 735	296106747	hypothetical protein	Ceg19
68	lpa_01748	1359574..1360152 [-], 579	296106751	hypothetical protein	lpa_01748
69	lpa_01776	1376301..1377254 [-], 954	296106768	periplasmic ligand-binding sensor domain protein	lpa_01776
70	lpa_01785	1382683..1383156 [-], 474	296106774	hypothetical protein	CegC3
71	lpa_01787	1383449..1385758 [+], 2310	296106775	hypothetical protein	Lem7
72	lpa_01789 (rimI)	1387430..1387933 [+], 504	296106777	Acetyltransferase	lpa_01789
73	lpa_01790	1388250..1389734 [+], 1485	296106778	hypothetical protein	lpa_01790
74	lpa_01795	1392406..1393230 [-], 825	296106781	hypothetical protein	RavP
75	lpa_01797	1395189..1396271 [+], 1083	296106783	hypothetical protein	RavQ
76	lpa_01802	1400765..1401487 [+], 723	296106787	hypothetical protein	lpa_01802
77	lpa_01819	1410846..1412834 [-], 1989	296106797	hypothetical protein	RavR
78	lpa_01826	1418734..1419153 [+], 420	296106803	bile acid beta-glucosidase	lpa_01826
79	lpa_01839 (hdeD)	1432408..1434267 [+], 1860	296106815	hypothetical protein	RavS
80	lpa_01899 (rssA)	1477049..1478788 [+], 1740	296106859	esterase of the alpha-beta hydrolase superfamily	VpdB
81	lpa_01901	1479441..1480505 [+], 1065	296106860	hypothetical protein	lpa_01901
82	lpa_01958	1531082..1533478 [-], 2397	296106906	thaumatin domain-containing protein	LegT
83	lpa_02090 (rssA)	1639102..1641753 [+], 2652	296107006	esterase of the alpha-beta hydrolase superfamily	VpdC
84	lpa_02119	1665288..1665806 [+], 519	296107032	hypothetical protein	lpa_02119
85	lpa_02161	1699023..1700600 [+], 1578	296107062	serine/threonine-protein kinase	LegK1
86	lpa_02162	1700654..1701403 [-], 750	296107063	hypothetical protein	lpa_02162
87	lpa_02168	1703562..1706087 [+], 2526	296107067	hypothetical protein	Lgt3/legc5
88	lpa_02169	1706297..1707322 [+], 1026	296107068	hypothetical protein	RavX
89	lpa_02185 (gph)	1713597..1715405 [+], 1809	296107078	phosphatase	Lem10
90	lpa_02253	1776247..1777023 [-], 777	296107133	membrane-associated HD superfamily hydrolase	RavY
91	lpa_02292	1805640..1806053 [-], 414	296107162	hypothetical protein	lpa_02292
92	lpa_02305	1817885..1819903 [-], 2019	296107173	hypothetical protein	LegC6
93	lpa_02317	1827799..1828860 [-], 1062	296107184	hypothetical protein	Lem11
94	lpa_02318	1829244..1830530 [-], 1287	296107185	hypothetical protein	LegL2
95	lpa_02346	1852322..1853641 [-], 1320	296107199	hypothetical protein	Ceg23
96	lpa_02350	1857021..1857416 [-], 396	296107203	hypothetical protein	Lem23
97	lpa_02367	1874069..1875328 [-], 1260	296107218	hypothetical protein	lpa_02367
98	lpa_02371 (sidB)	1878231..1879484 [-], 1254	296107221	SidB	SidB
99	lpa_02390	1894225..1895340 [-], 1116	296107234	hypothetical protein	lpa_02390
100	lpa_02398	1901752..1903233 [+], 1482	296107240	hypothetical protein	LegL3
101	lpa_02399	1903327..1904445 [-], 1119	296107241	hypothetical protein	lpa_02399
102	lpa_02408	1914462..1915823 [-], 1362	296107246	hypothetical protein	lpa_02408
103	lpa_02409	1916026..1917393 [-], 1368	296107247	hypothetical protein	lpa_02409
104	lpa_02413	1922073..1922963 [+], 891	296107251	hypothetical protein	lpa_02413
105	lpa_02431	1938528..1940036 [+], 1509	296107265	hypothetical protein	RavZ
106	lpa_02432	1940340..1941737 [+], 1398	296107266	hypothetical protein	lpa_02432
107	lpa_02433	1941748..1942617 [-], 870	296107267	hypothetical protein	lpa_02433
108	lpa_02437	1943204..1944406 [-], 1203	296107269	hypothetical protein	MavA
109	lpa_02439	1945871..1946497 [+], 627	296107271	Integral membrane protein (PIN domain superfamily)	lpa_02439
110	lpa_02442	1951210..1952520 [+], 1311	296107274	hypothetical protein	lpa_02442
111	lpa_02455	1960486..1962162 [+], 1677	296107281	kinectin 1 (kinesin receptor)	PpeA/legC3
112	lpa_02456	1962298..1963938 [+], 1641	296107282	hypothetical protein	PpeB
113	lpa_02474	1978128..1978586 [+], 459	296107298	Permease	lpa_02474
114	lpa_02484	1984264..1985949 [-], 1686	296107304	eukaryotic huntingtin interacting protein B	AnkI/legAS4
115	lpa_02538	2027851..2029161 [-], 1311	296107342	hypothetical protein	lpa_02538
116	lpa_02539	2029174..2029782 [-], 609	296107343	hypothetical protein	lpa_02539
117	lpa_02570	2059210..2059695 [+], 486	296107367	hypothetical protein	lpa_02570
118	lpa_02599	2078942..2080183 [+], 1242	296107389	hypothetical protein	RvfA
119	lpa_02600	2080368..2081564 [-], 1197	296107390	hypothetical protein	MarB
120	lpa_02606	2086482..2087417 [-], 936	296107395	hypothetical protein	lpa_02606
121	lpa_02652	2131057..2132475 [+], 1419	296107428	coiled coil domain protein	Ceg25
122	lpa_02675	2147482..2148144 [-], 663	296107444	hypothetical protein	Lem14
123	lpa_02714	2182422..2183639 [+], 1218	296107470	microtubule binding protein, putative	YlfB/legC2
124	lpa_02723	2186894..2188225 [-], 1332	296107475	hypothetical protein	lpa_02723
125	lpa_02726	2189483..2191144 [-], 1662	296107477	hypothetical protein	LegLC8
126	lpa_02762	2212169..2213974 [-], 1806	296107499	hypothetical protein	lpa_02762
127	lpa_02783	2232305..2235097 [+], 2793	296107516	hypothetical protein	lpa_02783
128	lpa_02811	2257209..2257823 [-], 615	296107539	hypothetical protein	Lem15
129	lpa_02835	2276080..2276259 [+], 180	296107555	hypothetical protein	Lem16
130	lpa_02837	2276433..2277767 [-], 1335	296107556	hypothetical protein	Lem17
131	lpa_02838	2278001..2279197 [+], 1197	296107557	guanine nucleotide exchange protein	RalF
132	lpa_02842	2281579..2283813 [-], 2235	296107560	coiled-coil-containing protein	LegC4
133	lpa_02857	2293964..2295958 [+], 1995	296107570	hypothetical protein	lpa_02857
134	lpa_02859	2296143..2296916 [+], 774	296107571	hypothetical protein	LirA
135	lpa_02861 (ppiB)	2298623..2299189 [+], 567	296107573	peptidyl-prolyl isomerase	LirB
136	lpa_02863	2299658..2301367 [-], 1710	296107575	hypothetical protein	PieA/lirC
137	lpa_02865	2301725..2304658 [-], 2934	296107576	hypothetical protein	PieC/lirE
138	lpa_02867	2305087..2306652 [-], 1566	296107577	hypothetical protein	PieD/lirF
139	lpa_02874	2309565..2311358 [-], 1794	296107582	hypothetical protein	PieE
140	lpa_02884	2316179..2316559 [-], 381	296107588	hypothetical protein	PieF
141	lpa_02889	2319234..2320676 [+], 1443	296107591	UVB-resistance protein UVR8	PieG/legG1
142	lpa_02892	2321519..2323414 [-], 1896	296107593	teichoic acid biosynthesis protein	SetA
143	lpa_02898	2328993..2331842 [-], 2850	296107598	hypothetical protein	lpa_02898
144	lpa_02992	2397064..2398122 [-], 1059	296107666	hypothetical protein	lpa_02992
145	lpa_03018	2417493..2418245 [-], 753	296107686	hypothetical protein	Ceg3
146	lpa_03037	2429099..2433565 [-], 4467	296107698	Sid -like protein	LaiA/SdeA
147	lpa_03060	2453511..2455139 [-], 1629	296107714	calmodulin-dependent protein kinase	LegK2
148	lpa_03071 (pof1)	2459929..2460447 [+], 519	296107722	F-box protein	AnkB/legAU13/ceg27
149	lpa_03076	2463751..2465199 [+], 1449	296107725	hypothetical protein	MavC
150	lpa_03077	2465377..2466555 [+], 1179	296107726	hypothetical protein	lpa_03077
151	lpa_03078	2466643..2467002 [+], 360	296107727	hypothetical protein	lpa_03078
152	lpa_03083	2470956..2475563 [+], 4608	296107731	SdeC protein	SdeC
153	lpa_03086	2475777..2476898 [+], 1122	296107732	Sid -like protein	SdeC
154	lpa_03087	2476952..2479579 [+], 2628	296107733	hypothetical protein	SidJ
155	lpa_03088	2479856..2485621 [+], 5766	296107734	substrates of the Legionella pneumophila Dot/Icm system SdeB	SdeB
156	lpa_03100	2512350..2513717 [-], 1368	296107743	hypothetical protein	lpa_03100
157	lpa_03107	2519204..2520454 [+], 1251	296107748	hypothetical protein	Lem19
158	lpa_03118	2527099..2528916 [-], 1818	296107756	sphinganine-1-phosphate aldolase	LegS2
159	lpa_03157	2572917..2573498 [-], 582	296107783	hypothetical protein	CegC4
160	lpa_03158	2573714..2574250 [-], 537	296107784	hypothetical protein	CegC4
161	lpa_03166	2580674..2581774 [+], 1101	296107791	hypothetical protein	WipC
162	lpa_03179	2590264..2591859 [-], 1596	296107801	ankyrin repeat-containing protein	LegA2
163	lpa_03180	2591880..2593652 [-], 1773	296107802	purine NTPase, putative	Lem20
164	lpa_03191	2600285..2601412 [-], 1128	296107809	TPR repeat protein, protein-protein interaction	LpnE
165	lpa_03196	2602693..2603928 [-], 1236	296107811	hypothetical protein	lpa_03196
166	lpa_03237	2638787..2641021 [-], 2235	296107839	hypothetical protein	Lem21
167	lpa_03268	2664037..2664687 [+], 651	296107862	hypothetical protein	lpa_03268
168	lpa_03296	2685405..2686682 [-], 1278	296107885	inclusion membrane protein A	YlfA/legC7
169	lpa_03298 (arp)	2690064..2691467 [-], 1404	296107887	Ankyrin repeat protein	AnkH/legA3, ankW
170	lpa_03312	2699495..2702938 [-], 3444	296107898	interaptin	Ceg28
171	lpa_03328 (arp)	2712551..2714476 [-], 1926	296107911	ankyrin	AnkK/legA5
172	lpa_03335	2720412..2721290 [+], 879	296107916	hypothetical protein	lpa_03335
173	lpa_03336	2721553..2721936 [+], 384	296107917	hypothetical protein	Lem22
174	lpa_03355	2735761..2736276 [+], 516	296107934	hypothetical protein	MavE
175	lpa_03367	2744193..2745044 [+], 852	296107943	hypothetical protein	MavF
176	lpa_03376	2752344..2752787 [+], 444	296107951	hypothetical protein	lpa_03376
177	lpa_03446 (dhlC)	2793676..2795130 [+], 1455	296108000	symporter	lpa_03446
178	lpa_03485	2822070..2823374 [-], 1305	296108023	SdbC	SdbC
179	lpa_03486	2823635..2824519 [+], 885	296108024	hypothetical protein	LegL6
180	lpa_03506	2837504..2838601 [+], 1098	296108039	hypothetical protein	Lem23
181	lpa_03507	2838783..2839046 [-], 264	296108040	hypothetical protein	lpa_03507
182	lpa_03511	2840295..2841113 [+], 819	296108042	hypothetical protein	Ceg29
183	lpa_03513	2843290..2845287 [+], 1998	296108044	patatin-like phospholipase	VpdA
184	lpa_03515	2845390..2846217 [-], 828	296108045	hypothetical protein	Lem24
185	lpa_03527	2849895..2850878 [+], 984	296108051	ankyrin repeat family protein	LegA1
186	lpa_03529	2851083..2851634 [+], 552	296108052	hypothetical protein	lpa_03529
187	lpa_03530	2851732..2854065 [-], 2334	296108053	hypothetical protein	Lem25
188	lpa_03532	2856104..2857447 [-], 1344	296108055	hypothetical protein	MavG
189	lpa_03537	2858518..2859318 [-], 801	296108057	hypothetical protein	MavH
190	lpa_03548	2866758..2868542 [+], 1785	296108065	hypothetical protein	Ceg30
191	lpa_03550	2868914..2869303 [+], 390	296108066	hypothetical protein	lpa_03550
192	lpa_03562	2875941..2876474 [-], 534	296108076	hypothetical protein	lpa_03562
193	lpa_03563	2876719..2877270 [-], 552	296108077	hypothetical protein	MavI
194	lpa_03574 (arp)	2881211..2883976 [-], 2766	296108083	Ankyrin repeat protein	AnkF/legA14/ceg31
195	lpa_03583	2886939..2888360 [+], 1422	296108089	hypothetical protein	AnkD/legA15
196	lpa_03589	2891437..2892054 [+], 618	296108094	hypothetical protein	lpa_03589
197	lpa_03615	2908839..2910185 [+], 1347	296108114	SdbB protein	SdbB
198	lpa_03628	2917425..2921309 [+], 3885	296108123	LepB protein	LepB
199	lpa_03646	2930092..2930985 [+], 894	296108135	helicase	MavJ
200	lpa_03658	2935516..2938344 [+], 2829	296108142	hypothetical protein	SidI/ceg32
201	lpa_03659	2938364..2939236 [+], 873	296108143	hypothetical protein	lpa_03659
202	lpa_03666	2943984..2944466 [-], 483	296108148	hypothetical protein	SdjA
203	lpa_03667 (sdeD)	2944538..2945686 [-], 1149	296108149	SdeD protein	SdeD
204	lpa_03668	2945983..2948706 [+], 2724	296108150	SdcA	SdcA
205	lpa_03669 (sidC)	2948869..2951598 [+], 2730	296108151	SidC Protein	SidC
206	lpa_03687	2964035..2965276 [-], 1242	296108164	hypothetical protein	MavL
207	lpa_03688	2965740..2967362 [-], 1623	296108165	hypothetical protein	LnaB
208	lpa_03692	2969407..2971101 [-], 1695	296108167	hypothetical protein	Lem27
209	lpa_03706	2979436..2980782 [-], 1347	296108179	hypothetical protein	lpa_03706
210	lpa_03707	2981433..2981801 [-], 369	296108180	hypothetical protein	lpa_03707
211	lpa_03710	2982551..2983378 [-], 828	296108182	hypothetical protein	lpa_03710
212	lpa_03727	2988559..2989956 [-], 1398	296108191	hypothetical protein	lpa_03727
213	lpa_03738	2995021..2996688 [-], 1668	296108198	hypothetical protein	lpa_03738
214	lpa_03743	2999448..3000290 [-], 843	296108201	hypothetical protein	lpa_03743
215	lpa_03745 (sPS1)	3000890..3002278 [+], 1389	296108202	Serine/threonine protein kinase	LegK3
216	lpa_03768	3016539..3017264 [-], 726	296108218	hypothetical protein	MavM
217	lpa_03779 (sidF)	3023998..3026736 [+], 2739	296108226	SidF	SidF
218	lpa_03784	3030578..3031735 [-], 1158	296108230	acid sphingomyelinase-like phosphodiesterase	LegS1
219	lpa_03790 (cutC)	3035340..3035843 [-], 504	296108235	hypothetical protein	Ceg33
220	lpa_03807	3047890..3049131 [+], 1242	296108247	hypothetical protein	Lem28
221	lpa_03846	3080165..3080917 [-], 753	296108273	membrane protein	lpa_03846
222	lpa_03859	3089245..3090456 [-], 1212	296108282	hypothetical protein	lpa_03859
223	lpa_03861	3090808..3092190 [-], 1383	296108283	hypothetical protein	MavV
224	lpa_03929	3155626..3156156 [+], 531	296108338	hypothetical protein	lpa_03929
225	lpa_03931	3157152..3157982 [-], 831	296108340	phytanoyl-CoA dioxygenase	LegD1
226	lpa_03966	3181924..3183486 [-], 1563	296108368	hypothetical protein	WipA
227	lpa_03968	3184897..3185928 [+], 1032	296108370	cNMP binding domain-containing protein	LegN
228	lpa_04004	3207705..3208874 [-], 1170	296108396	hypothetical protein	lpa_04004
229	lpa_04005	3209035..3210954 [-], 1920	296108397	hypothetical protein	lpa_04005
230	lpa_04063	3256362..3259808 [+], 3447	296108435	interaptin	LepA
231	lpa_04076	3271658..3272971 [-], 1314	296108446	hypothetical protein	Lem29
232	lpa_04089	3288839..3290821 [+], 1983	296108457	hypothetical protein	MavN
233	lpa_04104	3303144..3304745 [-], 1602	296108470	hypothetical protein	Ceg34
234	lpa_04109	3307928..3309184 [+], 1257	296108475	hypothetical protein	lpa_04109
235	lpa_04110	3309284..3310924 [-], 1641	296108476	hypothetical protein	lpa_04110
236	lpa_04133	3323961..3325160 [-], 1200	296108488	hypothetical protein	lpa_04133
237	lpa_04176	3357190..3358074 [-], 885	296108521	hypothetical protein	lpa_04176
238	lpa_04186	3363602..3365245 [-], 1644	296108527	hypothetical protein	lpa_04186
239	lpa_04193	3369752..3370489 [-], 738	296108532	hypothetical protein	lpa_04193
240	lpa_04199	3373836..3375749 [+], 1914	296108536	hypothetical protein	lpa_04199
241	lpa_04255	3407811..3409298 [+], 1488	296108578	hypothetical protein	lpa_04255
242	lpa_04293	3431913..3432641 [-], 729	296108607	hypothetical protein	lpa_04293
243	lpa_04300	3436370..3437554 [-], 1185	296108612	hypothetical protein	lpa_04300
244	lpa_04358	3480184..3482799 [-], 2616	296108655	hypothetical protein	lpa_04358
245	lpa_04395	3509133..3509933 [+], 801	296108681	hypothetical protein	LegP
246	lpa_04397	3509930..3511768 [-], 1839	296108682	hypothetical protein	lpa_04397

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	IcmS (296105993)	C-terminal fragment	-	The IcmS adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.	(1) PubMed: 28714967
2	IcmS/IcmW (chaperones)	CT Val-2 and Leu-3 required for translocation	AnkB	The IcmSW chaperone complex is essential for translocation of AnkB.	(2) PubMed: 18811729
3	IcmS/IcmW (chaperones)	C-terminal hydrophbic tail, CT20aa	sidG	The IcmSW complex interacts with a central region of the SidG protein. It resulted in a conformational change in the SidG protein as determined by differences in protease sensitivity in vitro.	(3) PubMed: 18069892
4	IcmS/IcmW (chaperones)	C-terminal hydrophobic tail, CT100aa sufficient	sidC	IcmSW could enhance effector protein translocation by mediating a conformational change.	(4) PubMed: 16714592
5	IcmS/IcmW (chaperones)	C-terminal hydrophobic tail. CT30aa sufficient	legS2	LegS2 translocation depends on a functional IcmS IcmW complex.	(5) PubMed: 19438520
6	IcmW (296108334)	C-terminal fragment	-	The IcmW adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.	(6) PubMed: 19946141
7	LvgA (296106117)	C-terminal fragment	-	The LvgA adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.	(7) PubMed: 28714967

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967]

(2) Al-Khodor S; Price CT; Habyarimana F; Kalia A; Abu Kwaik Y (2008). A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 70(4):908-23. [PudMed:18811729]

(3) Cambronne ED; Roy CR (2007). The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation. PLoS Pathog. 3(12):e188. [PudMed:18069892]

(4) VanRheenen SM; Luo ZQ; O'Connor T; Isberg RR (2006). Members of a Legionella pneumophila family of proteins with ExoU (phospholipase A) active sites are translocated to target cells. Infect Immun. 74(6):3597-606. [PudMed:16714592]

(5) Degtyar E; Zusman T; Ehrlich M; Segal G (2009). A Legionella effector acquired from protozoa is involved in sphingolipids metabolism and is targeted to the host cell mitochondria. Cell Microbiol. 11(8):1219-35. [PudMed:19438520]

(6) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]

(7) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967]

(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087]

(2) D'Auria G; Jimenez-Hernandez N; Peris-Bondia F; Moya A; Latorre A (2010). Legionella pneumophila pangenome reveals strain-specific virulence factors. BMC Genomics. 0.584027778. [PudMed:20236513]

This literature contains bioinformatics investigation