SecReT4
T4SS ID40
StrainLegionella pneumophila str. Paris
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_006368
Location550004..586375; 634498..635124; 3108400..3127151
NameDot/Icm
Functioneffector translocation
ClassificationType IVB; Type I
sequence This T4SS has been mentioned in a genome sequencing report.
structure Information of structure: structureLpSPL/Lpp2128, U-box 2 of LubX / LegU2 / Lpp2887

T4SS components
ComponentDotADotBDotCDotDIcmBIcmCIcmDIcmEIcmF
Number111112111
ComponentIcmGIcmHIcmJIcmKIcmLIcmMIcmNIcmOIcmP
Number111111111
ComponentIcmQIcmRIcmSIcmTIcmVIcmWIcmXLvgA
Number11111111

The information of T4SS components from NC_006368
Region 1: 550004..586375
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpp0504545657..547657 [+], 200154296473hypothetical protein 
2lpp0505548479..549255 [-], 77754296474hypothetical protein 
3lpp0506549621..549833 [+], 21354296475hypothetical protein 
4lpp0507 (icmT)550004..550264 [+], 26154296476hypothetical protein  IcmT
5lpp0508 (icmS)550265..550609 [+], 34554296477hypothetical protein  IcmSaccesspry protein
6lpp0509 (icmR)550709..551071 [+], 36354296478hypothetical protein  IcmR
7lpp0510 (icmQ)551162..551737 [+], 57654296479hypothetical protein  IcmQ
8lpp0511 (icmP/dotM)551924..553054 [+], 113154296480hypothetical protein  IcmP
9lpp0512 (icmO/dotL)553051..555402 [+], 235254296481hypothetical protein  IcmO
10lpp0513 (lphA)555806..556375 [+], 57054296482hypothetical protein  IcmN
11lpp0514 (icmM/dotJ)556387..556671 [+], 28554296483hypothetical protein  IcmM
12lpp0515 (icmL/dotI)556687..557325 [+], 63954296484hypothetical protein  IcmL
13lpp0516 (icmK/dotH)557328..558410 [+], 108354296485hypothetical protein  IcmK
14lpp0517 (icmE/dotG)558415..561561 [+], 314754296486hypothetical protein  IcmE
15lpp0518 (icmG/dotF)561576..562385 [+], 81054296487hypothetical protein  IcmG
16lpp0519 (icmC/dotE)562393..562977 [+], 58554296488hypothetical protein  IcmC
17lpp0520 (icmD)563003..563401 [+], 39954296489hypothetical protein  IcmD
18lpp0521 (icmJ/dotN)563599..564225 [+], 62754296490hypothetical protein  IcmJ
19lpp0522 (icmB/dotO)564261..567290 [+], 303054296491hypothetical protein  IcmB
20lpp0523 (citA/tphA)567388..568644 [-], 125754296492proline/betaine transport protein 
21lpp0524 (icmF)568647..571568 [-], 292254296493hypothetical protein  IcmF
22lpp0525 (dotU)571568..572353 [-], 78654296494hypothetical protein  IcmH
23lpp0526 (purH)572638..574227 [-], 159054296495bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase 
24lpp0527 (prmA)574254..575123 [-], 87054296496ribosomal protein L11 methyltransferase 
25lpp0528 (accC)575125..576468 [-], 134454296497biotin carboxylase (A subunit of acetyl-CoA carboxylase) 
26lpp0529 (accB)576481..576963 [-], 48354296498acetyl-CoA carboxylase biotin carboxyl carrier protein 
27lpp0530 (aroQ)576977..577414 [-], 438542964993-dehydroquinate dehydratase 
28lpp0531 (dcoA)577616..579406 [+], 179154296500pyruvate carboxylase subunit B 
29lpp0532 (proA)579972..581603 [+], 163254296501zinc metalloproteinase precursor 
30lpp0533581605..582453 [-], 84954296502hypothetical protein 
31lpp0534582903..583676 [+], 77454296503hypothetical protein 
32lpp0535583855..584865 [-], 101154296504hypothetical protein 
33lpp0536585015..585749 [-], 73554296505hypothetical protein 
34lpp0537 (dotV)585833..586375 [+], 54354296506hypothetical protein  IcmC
35lpp0538586519..586815 [-], 29754296507hypothetical protein 
36lpp0539 (pssA)587033..587770 [+], 73854296508hypothetical protein 
37lpp0540587999..588268 [-], 27054296509hypothetical protein 
38lpp0541588459..588758 [-], 30054296510hypothetical protein 
39lpp0542 (rpoN)588785..590179 [-], 139554296511RNA polymerase factor sigma-54 
40lpp0543 (rpmG)590387..590551 [-], 1655429651250S ribosomal protein L33 
41lpp0544 (rpmB)590566..590802 [-], 2375429651350S ribosomal protein L28 
 
Region 2: 634498..635124
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpp0582629527..629817 [-], 29154296551hypothetical protein 
2lpp0583629878..630369 [-], 49254296552hypothetical protein 
3lpp0584630329..631087 [-], 75954296553hypothetical protein 
4lpp0585631053..631661 [-], 60954296554hypothetical protein 
5lpp0586631658..632125 [-], 46854296555hypothetical protein 
6lpp0587632122..633120 [-], 99954296556hypothetical protein 
7lpp0588633117..633527 [-], 41154296557hypothetical protein 
8lpp0589633524..634087 [-], 56454296558hypothetical protein 
9lpp0590 (lvgA)634498..635124 [+], 62754296559hypothetical protein  LvgAaccesspry protein
10lpp0591635154..635531 [-], 37854296560hypothetical protein 
11lpp0592635681..636820 [-], 114054296561hypothetical protein 
12lpp0593 (sdhC)637141..637515 [+], 37554296562succinate dehydrogenase, cytochrome b556 subunit 
13lpp0594 (sdhD)637509..637856 [+], 34854296563succinate dehydrogenase, hydrophobic membrane anchor protein 
14lpp0595 (sdhA)637858..639627 [+], 177054296564succinate dehydrogenase flavoprotein subunit 
 
Region 3: 3108400..3127151
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpp27243104027..3105094 [-], 106854298660hypothetical protein 
2lpp27253105157..3106482 [+], 132654298661hypothetical protein 
3lpp27263106479..3107783 [+], 130554298662hypothetical protein 
4lpp27273107780..3108325 [+], 54654298663hypothetical protein 
5lpp2728 (dotD)3108400..3108891 [+], 49254298664lipoprotein DotD  DotD
6lpp2729 (dotC)3108872..3109783 [+], 91254298665defect in organelle trafficking lipoprotein DotC  DotC
7lpp2730 (dotB)3109783..3110916 [+], 113454298666defect in organelle trafficking protein DotB (ATPase)  DotB
8lpp27313110998..3112725 [+], 172854298667hypothetical protein 
9lpp27323112767..3113564 [-], 79854298668hypothetical protein 
10lpp27333113594..3114538 [-], 94554298669hypothetical protein 
11lpp27343114775..3115797 [+], 102354298670hypothetical protein 
12lpp27353116071..3116958 [+], 88854298671hypothetical protein 
13lpp27363117097..3117804 [+], 70854298672hypothetical protein 
14lpp2737 (dlpA)3117791..3119638 [+], 184854298673DlpA protein (isocitrate and isopropylmalate dehydrogenase family protein) 
15lpp27383119638..3120498 [+], 86154298674hypothetical protein 
16lpp2739 (sbpA)3120759..3121475 [-], 71754298675hypothetical protein 
17lpp2740 (dotA)3121606..3124713 [-], 310854298676defect in organelle trafficking protein DotA  DotA
18lpp2741 (icmV)3124710..3125165 [-], 45654298677intracellular multiplication protein IcmV  IcmV
19lpp2742 (icmW)3125278..3125733 [+], 45654298678intracellular multiplication protein IcmW  IcmWaccesspry protein
20lpp2743 (icmX)3125733..3127151 [+], 141954298679intracellular multiplication protein IcmX  IcmX
21lpp2744 (lphB)3127158..3128798 [-], 164154298680hypothetical protein 
22lpp27453129165..3131708 [+], 254454298681hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
RavA, CegC1, lpp0021, RavB, AnkQ/legA10, lpp0046, lpp0047, Ceg2, Ceg3, lpp0095, Lem1, Ceg4, VipF, CegC2, lpp0145, SdhB, cetLP1, RavD, RavC, LegU1, lpp0234, lpp0245, Ceg5, RavE, RavF, RavG, Ceg7, SidE/laiD, Ceg8, Ceg9, lpp0327, lpp0332, SdbA, LegG2, LtpM, Ceg10, Lem2, lpp0372, lpp0429, lpp0430, lpp0442, SdhA, LegA7/ceg11, AnkG/ankZ/legA7, lpp0471, LegY, AnkJ/legA11, Ceg14, Ceg15, AnkC/legA12, LegD2, lpp0581, SidA, lpp0688, WipB, AnkN/ankX legA8, Lem3, lpp0782, RavH, lpp0859, Ceg18, RavI, LidA, RavJ, LegL1, lpp1025, lpp1029, SidK, RavK, Lem4, lpp1105, RavL, RavM, Lem5, RavN, Ceg19, lpp1125, RavO, lpp1139, CegC3, Lem7, lpp1149, lpp1150, RavP, RavQ, lpp1160, RavR, lpp1173, RavS, VpdB, lpp1236, Lem8, LegT, SidG, EnhC homologue, VpdC, lpp1404, lpp1409, LegK1, lpp1440, Lgt3/legc5, RavX, Lem9, Lem10, RavY, LegC6, Lem11, LegL2, Ceg23, Lem23, lpp1609, lpp1625, LegL3, lpp1632, cetLP3, lpp1637, lpp1638, lpp1642, MavA, lpp1658, PpeA/legC3, PpeB, lpp1681, lpp1682, AnkI/legAS4/RomA, lpp1715, lpp1716, lpp1740, MarB, lpp1766, Ceg25, Lem14, YlfB/legC2, lpp1855, lpp1882, lpp1899, Lem15, Lem16, Lem17, RalF, LegC4, LegL5, lpp1941, LirA, LirB, PieD/lirF, PieE, PieF, PieG/legG1, SetA, lpp1967, lpp2033, LegK2, AnkB/legAU13/ceg27, MavC, lpp2087, lpp2088, SdeC, SdeC, SidJ, SdeB, LaiA/SdeA, lpp2099, Lem19, LegS2/LpSpl, CegC4, CegC4, WipC, LegA2, Lem20, LpnE, lpp2175, lpp2192, Lem21, lpp2225, YlfA/legC7, AnkH/legA3, ankW, Ceg28, AnkK/legA5, lpp2275, Lem22, MavE, MavF, lpp2308, lpp2417, lpp2418, lpp2444, SdbC, LegL6, Lem23, lpp2474, Ceg29, VpdA, Lem24, lpp2486, Lem25, MavG, MavH, Ceg30, lpp2501, lpp2510, MavI, AnkF/legA14/ceg31, AnkD/legA15, lpp2527, SdbB, LepB, MavJ, SidI/ceg32, lpp2573, SdjA, SdeD, SdcA, SidC, MavL, LnaB, Lem27, lpp2604, lpp2605, lpp2607, lpp2615, lpp2622, lpp2625, LegK3, MavM, SidF, LegS1, Ceg33, Lem28, lpp2681, lpp2690, MavV, lpp2746, LegD1, WipA, LegN, lpp2800, lpp2801, LepA, Lem29, VipE, MavN, lpp2882, LubX/legU2, VipD, lpp2889, lpp2903, lpp2933, lpp2938, lpp2943, lpp2944, lpp2947, lpp2980, lpp3004, lpp3009, lpp3047, LegP, lpp3072

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1lpp0008 11162..12421 [-], 126054295991hypothetical protein  RavA
2lpp0012 14880..16457 [-], 157854295995hypothetical protein  CegC1
3lpp0021 26665..27144 [+], 48054296004hypothetical proteinexperimental  lpp0021
4lpp0030 37645..38535 [-], 89154296013hypothetical protein  RavB
5lpp0037 44415..45932 [-], 151854296020hypothetical protein  AnkQ/legA10
6lpp0046 52728..52940 [-], 21354296029hypothetical protein  lpp0046
7lpp0047 53150..53548 [+], 39954296030hypothetical protein  lpp0047
8lpp0062 65555..66646 [+], 109254296045hypothetical protein  Ceg2
9lpp0094 97375..98142 [+], 76854296076hypothetical protein  Ceg3
10lpp0095 98132..99484 [-], 135354296077hypothetical protein  lpp0095
11lpp0104 107516..111490 [+], 397554296086hypothetical protein  Lem1
12lpp0110 117963..119156 [+], 119454296092hypothetical protein  Ceg4
13lpp0117 126393..127253 [-], 86154296098hypothetical protein  VipF
14lpp0140 153049..156360 [+], 331254296121hypothetical protein  CegC2
15lpp0145 161226..163694 [+], 246954296126hypothetical protein  lpp0145
16lpp0150 (sdhB)167114..172741 [-], 562854296131SdhB protein, substrate of the Dot/Icm system  SdhB
17lpp0155 178779..180113 [+], 133554296136hypothetical proteinexperimental  cetLP1
18lpp0224 249025..250002 [+], 97854296198hypothetical protein  RavD
19lpp0232 255591..256346 [-], 75654296206hypothetical proteinexperimental  RavC
20lpp0233 256612..257178 [-], 56754296207hypothetical protein  LegU1
21lpp0234 257395..258093 [+], 69954296208hypothetical protein  lpp0234
22lpp0245 269845..270756 [+], 91254296219hypothetical protein  lpp0245
23lpp0251 277434..278387 [+], 95454296225hypothetical protein  Ceg5
24lpp0253 280991..281995 [-], 100554296227hypothetical protein  RavE
25lpp0254 282282..283535 [-], 125454296228hypothetical protein  RavF
26lpp0269 303748..304335 [+], 58854296242hypothetical protein  RavG
27lpp0286 320813..321919 [+], 110754296259hypothetical protein  Ceg7
28lpp0304 (sidE)340555..345045 [-], 449154296275SidE protein, substrate of the Dot/Icm system  SidE/laiD
29lpp0310 351994..352752 [+], 75954296281hypothetical protein  Ceg8
30lpp0316 361030..361755 [+], 72654296287hypothetical protein  Ceg9
31lpp0327 371435..372427 [+], 99354296298hypothetical protein  lpp0327
32lpp0332 377546..377944 [+], 39954296303hypothetical protein  lpp0332
33lpp0349 (sdbA)393998..397348 [+], 335154296320SdbA protein, substrate of the Dot/Icm system  SdbA
34lpp0350 397428..398981 [+], 155454296321hypothetical protein  LegG2
35lpp0356 405129..407048 [+], 192054296327hypothetical proteinexperimental  LtpMinteraction
36lpp0360 410611..411735 [-], 112554296331hypothetical protein  Ceg10
37lpp0361 411972..412655 [+], 68454296332hypothetical protein  Lem2
38lpp0372 424006..424698 [+], 69354296343hypothetical protein  lpp0372
39lpp0429 477223..477528 [-], 30654296400hypothetical protein  lpp0429
40lpp0430 477704..480394 [-], 269154296401hypothetical protein  lpp0430
41lpp0442 487212..487811 [+], 60054296412hypothetical protein  lpp0442
42lpp0443 (sdhA)487869..492140 [-], 427254296413SdhA, substrate of the Dot/Icm system  SdhA
43lpp0468 513290..513952 [-], 66354296437hypothetical protein  LegA7/ceg11
44lpp0469 514226..515734 [+], 150954296438hypothetical protein  AnkG/ankZ/legA7
45lpp0471 517464..518054 [+], 59154296440hypothetical proteinexperimental  lpp0471
46lpp0489 533416..534723 [-], 130854296458hypothetical protein  LegY
47lpp0503 544586..545386 [-], 80154296472hypothetical protein  AnkJ/legA11
48lpp0504 545657..547657 [+], 200154296473hypothetical protein  Ceg14
49lpp0505 548479..549255 [-], 77754296474hypothetical protein  Ceg15
50lpp0547 592961..594448 [+], 148854296516hypothetical proteinexperimental  AnkC/legA12
51lpp0578 625653..626585 [+], 93354296547hypothetical protein  LegD2
52lpp0581 628692..629540 [-], 84954296550hypothetical protein  lpp0581
53lpp0675 (sidA)723866..725290 [-], 142554296644SidA protein, substrate of the Dot/Icm transport system  SidA
54lpp0688 740304..741641 [-], 133854296657hypothetical protein  lpp0688
55lpp0696 750356..751495 [+], 114054296665hypothetical protein  WipB
56lpp0750 838515..841352 [+], 283854296719hypothetical protein  AnkN/ankX legA8
57lpp0751 841359..843017 [-], 165954296720hypothetical protein  Lem3
58lpp0782 873953..874948 [+], 99654296743hypothetical protein  lpp0782
59lpp0799 892394..893818 [+], 142554296760hypothetical protein  RavH
60lpp0859 963489..964139 [+], 65154296818hypothetical protein  lpp0859
61lpp0959 1065633..1066337 [-], 70554296918hypothetical protein  Ceg18
62lpp0988 1093909..1094916 [-], 100854296947hypothetical protein  RavI
63lpp1002 (lidA)1109818..1112007 [-], 219054296961LidA protein, substrate of the Dot/Icm system  LidA
64lpp1006 1117259..1118434 [-], 117654296965hypothetical protein  RavJ
65lpp1007 1118596..1119483 [-], 88854296966hypothetical protein  LegL1
66lpp1025 1146772..1148013 [-], 124254296984hypothetical protein  lpp1025
67lpp1029 1152813..1153364 [-], 55254296988hypothetical protein  lpp1029
68lpp1030 1153690..1155384 [-], 169554296989hypothetical protein  SidK
69lpp1031 1155852..1156592 [+], 74154296990hypothetical protein  RavK
70lpp1101 1219613..1220581 [+], 96954297056hypothetical protein  Lem4
71lpp1105 1223732..1224994 [-], 126354297060hypothetical protein  lpp1105
72lpp1108 1227429..1228310 [-], 88254297063hypothetical protein  RavL
73lpp1109 1228551..1230611 [-], 206154297064hypothetical protein  RavM
74lpp1111 1231171..1231866 [+], 69654297066hypothetical protein  Lem5
75lpp1112 1231960..1232598 [-], 63954297067hypothetical protein  RavN
76lpp1121 1244305..1245039 [+], 73554297076hypothetical protein  Ceg19
77lpp1125 1248354..1248944 [-], 59154297080hypothetical protein  lpp1125
78lpp1130 1256114..1257673 [+], 156054297085hypothetical protein  RavO
79lpp1139 1264987..1265940 [-], 95454297094hypothetical protein  lpp1139
80lpp1146 1271801..1272271 [-], 47154297101hypothetical protein  CegC3
81lpp1147 1272563..1274872 [+], 231054297102hypothetical protein  Lem7
82lpp1149 1276607..1277047 [+], 44154297104hypothetical protein  lpp1149
83lpp1150 1277368..1278852 [+], 148554297105hypothetical protein  lpp1150
84lpp1154 1282739..1283563 [-], 82554297109hypothetical protein  RavP
85lpp1156 1285562..1286644 [+], 108354297111hypothetical protein  RavQ
86lpp1160 1291137..1291871 [+], 73554297115hypothetical protein  lpp1160
87lpp1168 1299134..1301122 [-], 198954297123hypothetical protein  RavR
88lpp1173 1307020..1307439 [+], 42054297128hypothetical protein  lpp1173
89lpp1186 1322050..1323909 [+], 186054297141hypothetical protein  RavS
90lpp1235 1370107..1371846 [+], 174054297190hypothetical protein  VpdB
91lpp1236 1372499..1373563 [+], 106554297191hypothetical protein  lpp1236
92lpp1253 1389158..1390762 [-], 160554297208hypothetical protein  Lem8
93lpp1283 1426380..1428782 [-], 240354297238hypothetical protein  LegT
94lpp1309 (sidG)1459664..1462561 [-], 289854297264SidG protein, substrate of the Dot/Icm system  SidG
95lpp1310 1462790..1463920 [+], 113154297265hypothetical proteinexperimental  EnhC homologue
96lpp1381 1538766..1541417 [+], 265254297336hypothetical protein  VpdC
97lpp1404 1560625..1563237 [+], 261354297359hypothetical protein  lpp1404
98lpp1409 1566490..1567008 [+], 51954297364hypothetical protein  lpp1409
99lpp1439 1602311..1603900 [+], 159054297394hypothetical protein  LegK1
100lpp1440 1603954..1604703 [-], 75054297395hypothetical protein  lpp1440
101lpp1444 1606872..1609445 [+], 257454297399hypothetical protein  Lgt3/legc5
102lpp1445 1609655..1610680 [+], 102654297400hypothetical protein  RavX
103lpp1447 1612347..1613732 [+], 138654297402hypothetical protein  Lem9
104lpp1453 1618016..1619824 [+], 180954297408hypothetical protein  Lem10
105lpp1508 1680617..1681348 [-], 73254297463hypothetical protein  RavY
106lpp1546 1721515..1723533 [-], 201954297501hypothetical protein  LegC6
107lpp1556 1731428..1732489 [-], 106254297511hypothetical protein  Lem11
108lpp1567 1746200..1747495 [-], 129654297519hypothetical protein  LegL2
109lpp1591 1771686..1773005 [-], 132054297541hypothetical protein  Ceg23
110lpp1595 1776384..1776779 [-], 39654297545hypothetical protein  Lem23
111lpp1609 1793420..1794679 [-], 126054297559hypothetical protein  lpp1609
112lpp1625 1813578..1814693 [-], 111654297574hypothetical protein  lpp1625
113lpp1631 1821106..1822587 [+], 148254297580hypothetical protein  LegL3
114lpp1632 1822681..1823799 [-], 111954297581hypothetical proteinexperimental  lpp1632
115lpp1634 1824720..1825232 [+], 51354297583hypothetical proteinexperimental  cetLP3
116lpp1637 1833794..1835197 [-], 140454297586hypothetical protein  lpp1637
117lpp1638 1835358..1836725 [-], 136854297587hypothetical protein  lpp1638
118lpp1642 1841397..1842287 [+], 89154297591hypothetical protein  lpp1642
119lpp1656 1858242..1859444 [-], 120354297605hypothetical protein  MavA
120lpp1658 1860909..1861535 [+], 62754297607hypothetical protein  lpp1658
121lpp1666 1873027..1874706 [+], 168054297615hypothetical protein  PpeA/legC3
122lpp1667 1874842..1876482 [+], 164154297616hypothetical protein  PpeB
123lpp1681 1889494..1889940 [+], 44754297630hypothetical protein  lpp1681
124lpp1682 1890005..1891693 [-], 168954297631hypothetical protein  lpp1682
125lpp1683 1892084..1893769 [-], 168654297632hypothetical proteinexperimental  AnkI/legAS4/RomAinteraction
126lpp1715 1931139..1932452 [-], 131454297664hypothetical protein  lpp1715
127lpp1716 1932465..1933073 [-], 60954297665hypothetical proteinexperimental  lpp1716
128lpp1740 1962509..1962991 [+], 48354297689hypothetical protein  lpp1740
129lpp1761 1981301..1982494 [-], 119454297710hypothetical protein  MarB
130lpp1766 1987413..1988348 [-], 93654297715hypothetical protein  lpp1766
131lpp1799 2032026..2033444 [+], 141954297748hypothetical protein  Ceg25
132lpp1818 2050100..2050762 [-], 66354297767hypothetical protein  Lem14
133lpp1848 2092904..2094121 [+], 121854297797hypothetical protein  YlfB/legC2
134lpp1855 2099011..2100342 [-], 133254297804hypothetical protein  lpp1855
135lpp1882 2126214..2128019 [-], 180654297830hypothetical proteinexperimental  lpp1882
136lpp1899 2146325..2149117 [+], 279354297847hypothetical protein  lpp1899
137lpp1914 2166631..2167245 [-], 61554297859hypothetical protein  Lem15
138lpp1930 2184863..2185621 [+], 75954297875hypothetical protein  Lem16
139lpp1931 2185721..2187055 [-], 133554297876hypothetical protein  Lem17
140lpp1932 (ralF)2187286..2188482 [+], 119754297877RalF protein, translocated into host cells by the Dot/Icm system  RalF
141lpp1935 2190832..2193066 [-], 223554297880hypothetical protein  LegC4
142lpp1940 2198679..2200283 [+], 160554297885hypothetical protein  LegL5
143lpp1941 2200547..2202541 [+], 199554297886hypothetical protein  lpp1941
144lpp1942 2202725..2203498 [+], 77454297887hypothetical protein  LirA
145lpp1946 2206829..2207395 [+], 56754297891hypothetical protein  LirB
146lpp1947 2207576..2209132 [-], 155754297892hypothetical protein  PieD/lirF
147lpp1952 2212041..2213834 [-], 179454297897hypothetical protein  PieE
148lpp1955 2215573..2215950 [-], 37854297900hypothetical protein  PieF
149lpp1959 2218765..2220192 [+], 142854297904hypothetical protein  PieG/legG1
150lpp1961 2221035..2222930 [-], 189654297906hypothetical protein  SetA
151lpp1967 2229998..2232826 [-], 282954297912hypothetical protein  lpp1967
152lpp2033 2297567..2298625 [-], 105954297978hypothetical protein  lpp2033
153lpp2076 2354183..2355811 [-], 162954298019hypothetical protein  LegK2
154lpp2082 2360600..2361064 [+], 46554298025hypothetical proteinexperimental  AnkB/legAU13/ceg27interaction
155lpp2086 2365217..2366665 [+], 144954298029hypothetical protein  MavC
156lpp2087 2366762..2368021 [+], 126054298030hypothetical protein  lpp2087
157lpp2088 2368109..2368468 [+], 36054298031hypothetical protein  lpp2088
158lpp2092 (sdeC)2372425..2377026 [+], 460254298035SdeC protein, substrate of the Dot/Icm system  SdeC
159lpp2093 2377240..2378361 [+], 112254298036hypothetical protein  SdeC
160lpp2094 2378414..2381038 [+], 262554298037hypothetical protein  SidJ
161lpp2095 (sdeB)2381318..2387080 [+], 576354298038hypothetical protein  SdeB
162lpp2096 (sdeA)2387451..2392088 [+], 463854298039hypothetical protein  LaiA/SdeA
163lpp2099 2394630..2395997 [-], 136854298042hypothetical protein  lpp2099
164lpp2104 2401466..2402725 [+], 126054298047hypothetical protein  Lem19
165lpp2128 2421091..2422908 [-], 181854298071hypothetical proteinexperimental  LegS2/LpSplinteraction
166lpp2149 2463867..2464448 [-], 58254298092hypothetical protein  CegC4
167lpp2150 2464665..2465201 [-], 53754298093hypothetical protein  CegC4
168lpp2157 2471646..2472746 [+], 110154298100hypothetical protein  WipC
169lpp2166 2481235..2482830 [-], 159654298109hypothetical protein  LegA2
170lpp2167 2482851..2484623 [-], 177354298110hypothetical protein  Lem20
171lpp2174 2496579..2497700 [-], 112254298117hypothetical protein  LpnE
172lpp2175 2497962..2499185 [-], 122454298118hypothetical protein  lpp2175
173lpp2192 2520386..2524243 [+], 385854298135hypothetical protein  lpp2192
174lpp2202 2535795..2538029 [-], 223554298145hypothetical protein  Lem21
175lpp2225 2561033..2561683 [+], 65154298168hypothetical protein  lpp2225
176lpp2246 2582461..2583738 [-], 127854298188hypothetical protein  YlfA/legC7
177lpp2248 2587321..2588724 [-], 140454298190hypothetical proteinexperimental  AnkH/legA3, ankW
178lpp2259 2596741..2600256 [-], 351654298201hypothetical protein  Ceg28
179lpp2270 2609875..2611800 [-], 192654298212hypothetical proteinexperimental  AnkK/legA5
180lpp2275 2617733..2618611 [+], 87954298217hypothetical protein  lpp2275
181lpp2276 2618876..2619259 [+], 38454298218hypothetical protein  Lem22
182lpp2292 2633081..2633596 [+], 51654298234hypothetical protein  MavE
183lpp2300 2641511..2642362 [+], 85254298242hypothetical proteinexperimental  MavF
184lpp2308 (lporfX)2649786..2650229 [+], 44454298250hypothetical proteinexperimental  lpp2308
185lpp2417 2759103..2759459 [+], 35754298355hypothetical proteinexperimental  lpp2417
186lpp2418 2759500..2760432 [-], 93354298356hypothetical proteinexperimental  lpp2418
187lpp2444 2782135..2783574 [+], 144054298380hypothetical protein  lpp2444
188lpp2458 (sdbC)2803321..2804625 [-], 130554298394SdbC proteins, substrate of the Dot/Icm system  SdbC
189lpp2459 2804894..2805778 [+], 88554298395hypothetical protein  LegL6
190lpp2472 (lidE)2819942..2821039 [+], 109854298408hypothetical protein  Lem23
191lpp2474 2822250..2822513 [-], 26454298410hypothetical protein  lpp2474
192lpp2476 2823764..2824582 [+], 81954298412hypothetical protein  Ceg29
193lpp2479 2826758..2828743 [+], 198654298415hypothetical protein  VpdA
194lpp2480 2828846..2829673 [-], 82854298416hypothetical protein  Lem24
195lpp2486 2836545..2837183 [-], 63954298422hypothetical protein  lpp2486
196lpp2487 2837395..2839656 [-], 226254298423hypothetical protein  Lem25
197lpp2489 2841764..2843107 [-], 134454298425hypothetical protein  MavG
198lpp2491 2844283..2845083 [-], 80154298427hypothetical protein  MavH
199lpp2500 2854334..2856118 [+], 178554298436hypothetical protein  Ceg30
200lpp2501 2856394..2856879 [+], 48654298437hypothetical protein  lpp2501
201lpp2510 2863393..2863932 [-], 54054298446hypothetical protein  lpp2510
202lpp2511 2864177..2864728 [-], 55254298447hypothetical protein  MavI
203lpp2517 2868667..2871432 [-], 276654298453hypothetical protein  AnkF/legA14/ceg31
204lpp2522 2874405..2875712 [+], 130854298458hypothetical protein  AnkD/legA15
205lpp2527 2878902..2879519 [+], 61854298463hypothetical protein  lpp2527
206lpp2546 (sdbB)2897193..2898539 [+], 134754298482SdbB protein ( substrate of the Dot/Icm system)  SdbB
207lpp2555 (lepB)2905779..2909663 [+], 388554298491effector protein B, substrate of the Dot/Icm secretion system  LepB
208lpp2566 2917788..2918696 [+], 90954298502hypothetical protein  MavJ
209lpp2572 2923626..2926454 [+], 282954298508hypothetical protein  SidI/ceg32
210lpp2573 2926474..2927346 [+], 87354298509hypothetical protein  lpp2573
211lpp2576 2930154..2932577 [-], 242454298512hypothetical protein  SdjA
212lpp2577 (sdeD)2932649..2933797 [-], 114954298513SdeD protein (substrate of the Dot/Icm system)  SdeD
213lpp2578 (sdcA)2934089..2936779 [+], 269154298514SdcA protein, paralog of SidC (substrate of the Dot/Icm system)  SdcA
214lpp2579 (sidC)2936942..2939656 [+], 271554298515SidC protein (substrate of the Dot/Icm system)experimental  SidCinteraction
215lpp2591 2952111..2953355 [-], 124554298527hypothetical protein  MavL
216lpp2592 2953818..2955437 [-], 162054298528hypothetical protein  LnaB
217lpp2594 2957609..2959303 [-], 169554298530hypothetical protein  Lem27
218lpp2604 2967503..2968849 [-], 134754298540hypothetical protein  lpp2604
219lpp2605 2969502..2969909 [-], 40854298541hypothetical protein  lpp2605
220lpp2607 2970606..2971433 [-], 82854298543hypothetical protein  lpp2607
221lpp2615 2976501..2977898 [-], 139854298551hypothetical protein  lpp2615
222lpp2622 2983872..2985539 [-], 166854298558hypothetical protein  lpp2622
223lpp2625 2988299..2989141 [-], 84354298561hypothetical protein  lpp2625
224lpp2626 2989724..2991112 [+], 138954298562hypothetical protein  LegK3
225lpp2629 2993009..2993734 [-], 72654298565hypothetical protein  MavM
226lpp2637 (sidF)3000468..3003206 [+], 273954298573substrate of the Dot/Icm system  SidF
227lpp2641 3007047..3008204 [-], 115854298577hypothetical proteinexperimental  LegS1
228lpp2644 3011808..3012311 [-], 50454298580hypothetical proteinexperimental  Ceg33
229lpp2656 3024356..3025597 [+], 124254298592hypothetical protein  Lem28
230lpp2681 3056661..3057413 [-], 75354298617hypothetical protein  lpp2681
231lpp2690 3065741..3066952 [-], 121254298626hypothetical protein  lpp2690
232lpp2691 3067304..3068686 [-], 138354298627hypothetical protein  MavV
233lpp2746 3131959..3132489 [+], 53154298682hypothetical protein  lpp2746
234lpp2748 3133485..3134315 [-], 83154298684hypothetical protein  LegD1
235lpp2775 3156958..3158520 [-], 156354298711hypothetical protein  WipA
236lpp2777 3159931..3160962 [+], 103254298713hypothetical protein  LegN
237lpp2800 3181364..3182533 [-], 117054298736hypothetical protein  lpp2800
238lpp2801 3182694..3184613 [-], 192054298737hypothetical protein  lpp2801
239lpp2839 (lepA)3230473..3233832 [+], 336054298775effector protein A, substrate of the Dot/Icm secretion system  LepA
240lpp2850 3245682..3246995 [-], 131454298786hypothetical protein  Lem29
241lpp2865 3264230..3265090 [+], 86154298800hypothetical protein  VipE
242lpp2867 3266557..3268539 [+], 198354298802hypothetical proteinexperimental  MavNinteraction
243lpp2882 3286057..3287313 [+], 125754298817hypothetical protein  lpp2882
244lpp2887 3296181..3296903 [-], 72354298820hypothetical proteinexperimental  LubX/legU2interaction
245lpp2888 3297191..3299038 [+], 184854298821hypothetical protein  VipD
246lpp2889 3299095..3300735 [-], 164154298822hypothetical proteinexperimental  lpp2889
247lpp2903 3315416..3316705 [-], 129054298836hypothetical protein  lpp2903
248lpp2933 3347338..3348222 [-], 88554298866hypothetical protein  lpp2933
249lpp2938 3353750..3355483 [-], 173454298871hypothetical protein  lpp2938
250lpp2943 3359890..3360627 [-], 73854298876hypothetical protein  lpp2943
251lpp2944 3360929..3361471 [-], 54354298877hypothetical protein  lpp2944
252lpp2947 3363966..3365879 [+], 191454298880hypothetical proteinexperimental  lpp2947
253lpp2980 3397955..3399442 [+], 148854298913hypothetical protein  lpp2980
254lpp3004 3421637..3422371 [-], 7355429893716S ribosomal RNA methyltransferase RsmEexperimental  lpp3004
255lpp3009 3428505..3429689 [-], 118554298942hypothetical protein  lpp3009
256lpp3047 3467562..3470177 [-], 261654298980hypothetical proteinexperimental  lpp3047
257lpp3071 3496419..3497219 [+], 80154299004hypothetical protein  LegP
258lpp3072 3497216..3499054 [-], 183954299005hypothetical protein  lpp3072

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on structure of this T4ss

#NameImageResourceDetailReference
1LpSPL/Lpp21284W8I PDB (4W8I)Crystal structure of LpSPL/Lpp2128, Legionella pneumophila sphingosine-1 phosphate lyase.(1) PubMed: 26831115
2U-box 2 of LubX / LegU2 / Lpp28874XI1 PDB (4XI1)Crystal structure of U-box 2 of LubX / LegU2 / Lpp2887 from Legionella pneumophila str. Paris, wild-type.(2) PubMed: 26146184
(1) Rolando M et al. (2016). Legionella pneumophila S1P-lyase targets host sphingolipid metabolism and restrains autophagy. Proc Natl Acad Sci U S A. 113(7):1901-6. [PudMed:26831115] experimental
(2) Quaile AT et al. (2015). Molecular Characterization of LubX: Functional Divergence of the U-Box Fold by Legionella pneumophila. Structure. 23(8):1459-1469. [PudMed:26146184] experimental
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1IcmS (54296477)C-terminal fragment-The IcmS adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.(1) PubMed: 28714967
2IcmS/IcmW (chaperones)CT Val-2 and Leu-3 required for translocationAnkBThe IcmSW chaperone complex is essential for translocation of AnkB.(2) PubMed: 18811729
3IcmS/IcmW (chaperones)C-terminal hydrophbic tail, CT20aasidGThe IcmSW complex interacts with a central region of the SidG protein. It resulted in a conformational change in the SidG protein as determined by differences in protease sensitivity in vitro. (3) PubMed: 18069892
4IcmS/IcmW (chaperones)C-terminal hydrophobic tail, CT100aa sufficientsidCIcmSW could enhance effector protein translocation by mediating a conformational change. (4) PubMed: 16714592
5IcmS/IcmW (chaperones)C-terminal hydrophobic tail. CT30aa sufficientlegS2LegS2 translocation depends on a functional IcmS IcmW complex.(5) PubMed: 19438520
6IcmW (54298678)C-terminal fragment-The IcmW adaptors are necessary for translocation of different subsets of effectors through the Legionella Dot/Icm T4SS apparatus.(6) PubMed: 19946141
7LvgA (54296559)C-terminal fragment-The LvgA adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.(7) PubMed: 28714967
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967] experimental
(2) Al-Khodor S; Price CT; Habyarimana F; Kalia A; Abu Kwaik Y (2008). A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 70(4):908-23. [PudMed:18811729] experimental
(3) Cambronne ED; Roy CR (2007). The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation. PLoS Pathog. 3(12):e188. [PudMed:18069892] experimental
(4) VanRheenen SM; Luo ZQ; O'Connor T; Isberg RR (2006). Members of a Legionella pneumophila family of proteins with ExoU (phospholipase A) active sites are translocated to target cells. Infect Immun. 74(6):3597-606. [PudMed:16714592] experimental
(5) Degtyar E; Zusman T; Ehrlich M; Segal G (2009). A Legionella effector acquired from protozoa is involved in sphingolipids metabolism and is targeted to the host cell mitochondria. Cell Microbiol. 11(8):1219-35. [PudMed:19438520] experimental in_silico
(6) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]
(7) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1AnkB (54298025)ParvBmurineAnkB and ParvB were colocalized at the periphery of lamellipodia and can diminish ubiquitination of ParvB.(1) PubMed: 20345489experimental
2IroT/mavN (54298802)unknownunknownIroT/mavN is a membrane protein and is involved in ferrous iron transport.(2) PubMed: 25141909experimental
3LpSpl/LegS2 (54298071)Sphingosine-1-phosphate (S1P)murineLpSpl depletes host S1P to repress autophagy in the host cell. It also facilitate intracellular replication of Legionella pneumophila.(3) PubMed: 26831115experimental
4LtpM (54296327)Phosphatidylinositol 3-phosphatehumanLtpM is a type of glucosyltransferase activated by phosphoinositide and binds PI3P with high affinity.(4) PubMed: 30573678experimental
5LubX (54298820)Clk1 (Cdc2-like kinase 1)murineLubX has two domains which is similar to the U-box. It mediates polyubiquitination of host Clk1 and has activity of ubiquitin ligase.(5) PubMed: 18284575experimental
6RomA (54297632)histone H3human, amoebaeRomA localizes to nucleus of the infected cell, and methylates histone H3K14 to inhibit expression of host gene and facilitate efficient intracellular replication.(6) PubMed: 23601102experimental
7SidC (54298515)Phosphatidylinositol-4-phosphate (PtdIns4P)murineDuring the maturation of LCVs, SidC and SdcA play a part in three steps. The three steps are the interaction with early secretory vesicles, the recruiting of ER vesicles and the membrane dynamics of the LCVs,respectively.(7) PubMed: 18673369insolico
experimental This T4SE is mentioned in the literature.
insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Lomma M et al. (2010). The Legionella pneumophila F-box protein Lpp2082 (AnkB) modulates ubiquitination of the host protein parvin B and promotes intracellular replication. Cell Microbiol. 12(9):1272-91. [PudMed:20345489] experimental
(2) Portier E et al. (2015). IroT/mavN, a new iron-regulated gene involved in Legionella pneumophila virulence against amoebae and macrophages. Environ Microbiol. 17(4):1338-50. [PudMed:25141909] experimental
(3) Rolando M et al. (2016). Legionella pneumophila S1P-lyase targets host sphingolipid metabolism and restrains autophagy. Proc Natl Acad Sci U S A. 113(7):1901-6. [PudMed:26831115] experimental
(4) Levanova N et al. (2019). The Legionella effector LtpM is a new type of phosphoinositide-activated glucosyltransferase. J Biol Chem. 294(8):2862-2879. [PudMed:30573678] experimental
(5) Kubori T; Hyakutake A; Nagai H (2008). Legionella translocates an E3 ubiquitin ligase that has multiple U-boxes with distinct functions. Mol Microbiol. 67(6):1307-19. [PudMed:18284575] experimental
(6) Monica Rolando et al. (2013). Legionella pneumophila effector RomA uniquely modifies host chromatin to repress gene expression and promote intracellular bacterial replication. Cell Host Microbe. 13(4):395-405. [PudMed:23601102] experimental
(7) Ragaz C; Pietsch H; Urwyler S; Tiaden A; Weber SS; Hilbi H (2008). The Legionella pneumophila phosphatidylinositol-4 phosphate-binding type IV substrate SidC recruits endoplasmic reticulum vesicles to a replication-permissive vacuole. Cell Microbiol. 10(12):2416-33. [PudMed:18673369] experimental
(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] in_silico
(2) Doleans-Jordheim A; Akermi M; Ginevra C; Cazalet C; Kay E; Schneider D; Buchrieser C; Atlan D; Vandenesch F; Etienne J; Jarraud S (2006). Growth-phase-dependent mobility of the lvh-encoding region in Legionella pneumophila strain Paris. Microbiology. 152(Pt 12):3561-8. [PudMed:17159208] experimental
(3) Cazalet C; Rusniok C; Bruggemann H; Zidane N; Magnier A; Ma L; Tichit M; Jarraud S; Bouchier C; Vandenesch F; Kunst F; Etienne J; Glaser P; Buchrieser C (2004). Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Nat Genet. 36(11):1165-73. [PudMed:15467720]
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU