SecReT4
T4SS ID159
StrainCoxiella burnetii CbuG_Q212
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_011527
Location336565..370332
NameDot/Icm
Functioneffector translocation
ClassificationType IVB; Type I
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentDotADotBDotCDotDIcmBIcmCIcmDIcmEIcmF
Number111111110
ComponentIcmGIcmHIcmJIcmKIcmLIcmMIcmNIcmOIcmP
Number101120111
ComponentIcmQIcmRIcmSIcmTIcmVIcmWIcmXLvgA
Number10111110

The information of T4SS components from NC_011527
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1CbuG_0361332651..332770 [+], 120212212004hypothetical protein 
2CbuG_0362 (rfaE)332778..334268 [+], 1491212212005bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase 
3CbuG_0363334293..334475 [-], 183212212006virulence-associated protein C 
4CbuG_0364334600..334944 [+], 345212212007hypothetical protein 
5CbuG_0365334941..336035 [-], 1095212212008transposase 
6CbuG_0367 (icmX)336565..337695 [-], 1131212212009IcmX  IcmX
7CbuG_0368337711..338169 [-], 459212212010hypothetical membrane-associated protein 
8CbuG_0369 (icmW)338166..338615 [-], 450212212011IcmW  IcmW
9CbuG_0370 (icmV)338805..339299 [+], 495212212012IcmV  IcmV
10CbuG_0371 (dotA)339296..341740 [+], 2445212212013DotA  DotA
11CbuG_0372341795..341890 [+], 96212212014hypothetical protein 
12CbuG_0373342124..342396 [+], 273212212015hypothetical protein 
13CbuG_0374342393..343487 [-], 1095212212016transposase 
14CbuG_0375343589..344923 [-], 1335212212017glycine betaine transporter 
15CbuG_0376 (dotB)344930..346048 [-], 1119212212018ATP-binding protein  DotB
16CbuG_0377 (dotC)346086..346910 [-], 825212212019DotC  DotC
17CbuG_0378 (dotD)346913..347422 [-], 510212212020DotD  DotD
18CbuG_0379 (icmS)347579..347917 [-], 339212212021IcmS  IcmSaccesspry protein
19CbuG_0380 (icmT)347917..348174 [-], 258212212022IcmT  IcmT
20CbuG_0381348290..349384 [+], 1095212212023transposase 
21CbuG_0382349381..349716 [-], 336212212024hypothetical protein 
22CbuG_0384351177..352382 [+], 1206212212025ATPase 
23CbuG_0385352479..352616 [-], 138212212026hypothetical protein 
24CbuG_0387353978..354151 [-], 174212212027hypothetical protein 
25CbuG_0388354267..354416 [+], 150212212028hypothetical protein 
26CbuG_0389 (icmQ)354413..355129 [+], 717212212029IcmQ  IcmQ
27CbuG_0390 (icmP)355130..356293 [+], 1164212212030IcmP  IcmP
28CbuG_0391 (icmO)356269..358647 [+], 2379212212031IcmO  IcmO
29CbuG_0392 (icmN)358657..359295 [+], 639212212032IcmN protein, OmpA family  IcmN
30CbuG_0393 (icmL.1)359302..359925 [+], 624212212033IcmL  IcmL
31CbuG_0394 (icmL.2)359927..360583 [+], 657212212034IcmL  IcmL
32CbuG_0395 (icmK)360594..361649 [+], 1056212212035IcmK  IcmK
33CbuG_0396 (icmE)361636..364755 [+], 3120212212036IcmE  IcmE
34CbuG_0397 (icmG)364773..365507 [+], 735212212037IcmG  IcmG
35CbuG_0398 (icmC)365578..366087 [+], 510212212038IcmC  IcmC
36CbuG_0399 (icmD)366181..366597 [+], 417212212039IcmD  IcmD
37CbuG_0400 (icmJ)366610..367248 [+], 639212212040IcmJ  IcmJ
38CbuG_0401 (icmB)367321..370332 [+], 3012212212041IcmB  IcmB
39CbuG_0402370347..370484 [-], 138212212042hypothetical protein 
40CbuG_0403370702..371235 [-], 534212212043hypothetical protein 
41CbuG_0404371292..371645 [-], 354212212044hypothetical protein 
42CbuG_0405371775..371909 [+], 135212212045hypothetical protein 
43CbuG_0406371938..372552 [+], 615212212046ankyrin repeat protein 
44CbuG_0407372846..373229 [+], 384212212047hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes

Effectors
CbuG_0006, CbuG_0092, CbuG_0133, CBUG_0136, AnkM, AnkM, CbuG_0140, CbuG_0151, CbuG_0157, CbuG_0164, CBUG_0170, CBUG_0171, CbuG_0197, CbuG_0272, CbuG_0282, CbuG_0309, CBUG_0337, CBUG_0344, CbuG_0360, CbuG_0365, CbuG_0374, CbuG_0381, CBUG_0403, CBUG_0407, CbuG_0419, CBUG_0435, CBUG_0436, CbuG_0440, CBUG_0446, CbuG_0454, CbuG_0467, CbuG_0468, CbuG_0477, CbuG_0479, CbuG_0486, CbuG_0530, CbuG_0554, CBUG_0575, CbuG_0582, CbuG_0591, CbuG_0633, CbuG_0641, CbuG_0696, CbuG_0739, AnkI, CbuG_0793, AnkI, CBUG_0811, CbuG_0824, CBUG_0859, CBUG_0924, CbuG_0927, CbuG_0958, CbuG_0959, AnkH, CbuG_1068, CBUG_1117, CbuG_1121, CbuG_1199, CbuG_1207, AnkG, CBUG_1228, CBUG_1341, CBUG_1367, CbuG_1369, CbuG_1400, CbuG_1471, AnkN, CBUG_1498, CBUG_1544, AnkF, CbuG_1587, CbuG_1597, CbuG_1602, CbuG_1603, CbuG_1606, CbuG_1625, CbuG_1711, CbuG_1738, ElpA, CBUG_1805, CBUG_1822, CbuG_1837, CbuG_1886, CBUG_1899, CbuG_1937, CbuG_1938, CbuG_1943, CbuG_1970, CbuG_1971, CbuG_1996, CBUG_2016, CBUG_2022, CBUG_2025, CBUG_2044, CbuG_2057, CbuG_2064, CBUG_2077, CbuG_2079

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1CbuG_0006 6699..7793 [+], 1095212211674transposase  CbuG_0006
2CbuG_0092 78021..79115 [+], 1095212211756transposase  CbuG_0092
3CbuG_0133 121318..122580 [+], 1263212211793hypothetical protein  CbuG_0133
4CbuG_0136 124365..124961 [-], 597212211795hypothetical proteinexperimental  CBUG_0136
5CbuG_0137 124971..125096 [-], 126212211796hypothetical protein  AnkM
6CbuG_0139 126339..128735 [-], 2397212211798ankyrin repeat proteinexperimental  AnkM
7CbuG_0140 129016..130080 [+], 1065212211799transposase  CbuG_0140
8CbuG_0151 139597..140214 [-], 618212211810alpha/beta hydrolase  CbuG_0151
9CbuG_0157 145769..146863 [+], 1095212211816transposase  CbuG_0157
10CbuG_0164 154757..155851 [+], 1095212211822transposase  CbuG_0164
11CbuG_0170 160238..161104 [-], 867212211827gluconolactonaseexperimental  CBUG_0170
12CbuG_0171 161284..162966 [+], 1683212211828hypothetical proteinexperimental  CBUG_0171
13CbuG_0197 190298..190717 [+], 420212211853hypothetical protein  CbuG_0197
14CbuG_0272 254988..256082 [+], 1095212211921transposase  CbuG_0272
15CbuG_0282 261523..262776 [-], 1254212211929low-affinity inorganic phosphate transporter  CbuG_0282
16CbuG_0309 284924..286018 [+], 1095212211955transposase  CbuG_0309
17CbuG_0337 312104..313525 [-], 1422212211980hypothetical proteinexperimental  CBUG_0337
18CbuG_0344 318255..318689 [+], 435212211987hypothetical cytosolic proteinexperimental  CBUG_0344
19CbuG_0360 331527..332621 [-], 1095212212003transposase  CbuG_0360
20CbuG_0365 334941..336035 [-], 1095212212008transposase  CbuG_0365
21CbuG_0374 342393..343487 [-], 1095212212016transposase  CbuG_0374
22CbuG_0381 348290..349384 [+], 1095212212023transposase  CbuG_0381
23CbuG_0403 370702..371235 [-], 534212212043hypothetical proteinexperimental  CBUG_0403
24CbuG_0407 372846..373229 [+], 384212212047hypothetical proteinexperimental  CBUG_0407
25CbuG_0419 386543..387637 [+], 1095212212056transposase  CbuG_0419
26CbuG_0435 399563..399682 [+], 120212212072hypothetical proteinexperimental  CBUG_0435
27CbuG_0436 401071..401952 [-], 882212212073hypothetical membrane spanning proteinexperimental  CBUG_0436
28CbuG_0440 404683..405747 [+], 1065212212077transposase  CbuG_0440
29CbuG_0446 410242..410976 [-], 735212212082hypothetical proteinexperimental  CBUG_0446
30CbuG_0454 418055..419761 [-], 1707212212089hypothetical membrane spanning protein  CbuG_0454
31CbuG_0467 432926..433444 [+], 519212212100hypothetical protein  CbuG_0467
32CbuG_0468 433550..433693 [-], 144212212101hypothetical protein  CbuG_0468
33CbuG_0477 442221..443552 [-], 1332212212109hypothetical membrane spanning protein  CbuG_0477
34CbuG_0479 443733..444827 [+], 1095212212111transposase  CbuG_0479
35CbuG_0486 453467..454108 [-], 642212212117hypothetical protein  CbuG_0486
36CbuG_0530 493137..494231 [-], 1095212212158transposase  CbuG_0530
37CbuG_0554 515059..517071 [+], 2013212212178tetratricopeptide repeat family protein  CbuG_0554
38CbuG_0575 540611..541072 [+], 462212212196hypothetical proteinexperimental  CBUG_0575
39CbuG_0582 547166..548260 [-], 1095212212203transposase  CbuG_0582
40CbuG_0591 556630..557082 [+], 45321221221117 kDa common-antigen  CbuG_0591
41CbuG_0633 596477..598336 [+], 1860212212250protein kinase family  CbuG_0633
42CbuG_0641 605276..606370 [-], 1095212212257transposase  CbuG_0641
43CbuG_0696 662683..663399 [+], 717212212306hypothetical cytosolic protein  CbuG_0696
44CbuG_0739 707980..709074 [+], 1095212212345transposase  CbuG_0739
45CbuG_0789 761984..762577 [+], 594212212394hypothetical membrane spanning proteinexperimental  AnkI
46CbuG_0793 764794..765888 [-], 1095212212398transposase  CbuG_0793
47CbuG_0798 769864..772044 [-], 2181212212400ankyrin repeat protein  AnkI
48CbuG_0811 786309..786845 [-], 537212212412hypothetical cytosolic proteinexperimental  CBUG_0811
49CbuG_0824 799300..800394 [-], 1095212212425transposase  CbuG_0824
50CbuG_0859 833361..834047 [-], 687212212450hypothetical proteinexperimental  CBUG_0859
51CbuG_0924 898801..899397 [+], 597212212507hypothetical proteinexperimental  CBUG_0924
52CbuG_0927 900755..901849 [+], 1095212212510transposase  CbuG_0927
53CbuG_0958 934316..934429 [+], 114212212534hypothetical protein  CbuG_0958
54CbuG_0959 934499..936247 [-], 1749212212535hypothetical protein  CbuG_0959
55CbuG_0984 960202..960507 [-], 306212212552hypothetical protein  AnkH
56CbuG_1068 1031373..1032824 [-], 1452212212618hypothetical protein  CbuG_1068
57CbuG_1117 1075339..1076499 [-], 1161212212663hypothetical cytosolic proteinexperimental  CBUG_1117
58CbuG_1121 1080126..1080791 [+], 666212212667hypothetical cytosolic protein  CbuG_1121
59CbuG_1199 (rimI)1163944..1164318 [-], 375212212739ribosomal-protein-S18-alanine acetyltransferase  CbuG_1199
60CbuG_1207 1168873..1170330 [+], 1458212212746hypothetical protein  CbuG_1207
61CbuG_1220 1189264..1190280 [-], 1017212212757ankyrin repeat proteinexperimental  AnkGinteraction
62CbuG_1228 1194659..1195129 [-], 471212212764PhnBexperimental  CBUG_1228
63CbuG_1341 1303055..1304044 [-], 990212212858hypothetical proteinexperimental  CBUG_1341
64CbuG_1367 1324461..1325222 [+], 762212212882coenzyme PQQ synthesis protein Cexperimental  CBUG_1367
65CbuG_1369 1326177..1327667 [-], 1491212212884hypothetical membrane spanning protein  CbuG_1369
66CbuG_1400 1361962..1363056 [-], 1095212212912transposase  CbuG_1400
67CbuG_1471 1439686..1440780 [+], 1095212212979transposase  CbuG_1471
68CbuG_1487 1457953..1459542 [-], 1590212212992ankyrin repeat proteinexperimental  AnkN
69CbuG_1498 1466378..1467526 [-], 1149212213001fructose-1,6-bisphosphataseexperimental  CBUG_1498
70CbuG_1544 1509392..1509595 [+], 204212213047hypothetical proteinexperimental  CBUG_1544
71CbuG_1564 1526684..1527238 [+], 555212213067ankyrin repeat proteinexperimental  AnkF
72CbuG_1587 1542246..1543730 [+], 1485212213089hypothetical proteinexperimental  CbuG_1587
73CbuG_1597 1550757..1551920 [-], 1164212213097hypothetical cytosolic protein  CbuG_1597
74CbuG_1602 1553162..1554256 [-], 1095212213101transposase  CbuG_1602
75CbuG_1603 1554452..1556188 [+], 1737212213102hypothetical membrane spanning protein  CbuG_1603
76CbuG_1606 1557121..1557222 [+], 102212213105hypothetical protein  CbuG_1606
77CbuG_1625 1576165..1577259 [-], 1095212213120transposase  CbuG_1625
78CbuG_1711 (algC)1660737..1662152 [-], 1416212213196phosphoglucomutase  CbuG_1711
79CbuG_1738 1679967..1681187 [-], 1221212213222trans-2-enoyl-CoA reductase  CbuG_1738
80CbuG_1796 1725042..1726298 [-], 1257212213274hypothetical membrane spanning proteinexperimental  ElpA
81CbuG_1805 1733403..1733798 [+], 396212213281ankyrin repeat proteinexperimental  CBUG_1805
82CbuG_1822 1750978..1751172 [-], 195212213294hypothetical proteinexperimental  CBUG_1822
83CbuG_1837 1765860..1766600 [-], 741212213303serine/threonine protein kinase domain protein  CbuG_1837
84CbuG_1886 1815269..1816582 [-], 1314212213343hypothetical protein  CbuG_1886
85CbuG_1899 1827018..1827230 [+], 213212213356hypothetical proteinexperimental  CBUG_1899
86CbuG_1937 1859530..1860417 [-], 888212213391hypothetical transcriptional regulatory protein  CbuG_1937
87CbuG_1938 1860593..1861384 [-], 792212213392hypothetical membrane spanning protein  CbuG_1938
88CbuG_1943 1866082..1867176 [+], 1095212213397transposase  CbuG_1943
89CbuG_1970 1890490..1892622 [+], 2133212213421hypothetical protein  CbuG_1970
90CbuG_1971 1892811..1893422 [+], 612212213422hypothetical cytosolic protein  CbuG_1971
91CbuG_1996 1913951..1915045 [+], 1095212213447transposase  CbuG_1996
92CbuG_2016 1928157..1929341 [-], 1185212213467hypothetical proteinexperimental  CBUG_2016
93CbuG_2022 1935088..1936185 [-], 1098212213473hypothetical proteinexperimental  CBUG_2022
94CbuG_2025 1938573..1939169 [+], 597212213476hypothetical cytosolic proteinexperimental  CBUG_2025
95CbuG_2044 1955417..1955611 [-], 195212213494hypothetical proteinexperimental  CBUG_2044
96CbuG_2057 1971797..1972441 [-], 645212213502hypothetical protein  CbuG_2057
97CbuG_2064 1981226..1981363 [-], 138212213507hypothetical protein  CbuG_2064
98CbuG_2077 1991924..1992241 [+], 318212213519hypothetical proteinexperimental  CBUG_2077
99CbuG_2079 1993183..1994229 [-], 1047212213521Fic family protein  CbuG_2079

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1IcmS (chaperone) (212212021)NDAnkB, AnkH, AnkI, AnkJ, AnkMThe chaperone IcmS is essential for translocation into the host cytosol.(1) PubMed: 19411324
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1AnkG (212212757)gC1qR (p32)human, murineAnkG interacts with gC1qR(p32) to regulate an anti-apoptotic pathway which is required for adaptation to mammalian hosts of Coxiella.(1) PubMed: 20944063insolico

insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Luhrmann A; Nogueira CV; Carey KL; Roy CR (2010). Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. Proc Natl Acad Sci U S A. 107(44):18997-9001. [PudMed:20944063] experimental
(1) McDonough JA; Newton HJ; Roy CR (2012). Coxiella burnetii Secretion Systems. Adv Exp Med Biol. 984:171-97. [PudMed:22711632]
(2) Chen C; Banga S; Mertens K; Weber MM; Gorbaslieva I; Tan Y; Luo ZQ; Samuel JE (2010). Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii. Proc Natl Acad Sci U S A. 107(50):21755-60. [PudMed:21098666] experimental
(3) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
(4) Pan X; Luhrmann A; Satoh A; Laskowski-Arce MA; Roy CR (2008). Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Science. 320(5883):1651-4. [PudMed:18566289] experimental in_silico
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU