SecReT4
T4SS ID158
StrainCoxiella burnetii CbuK_Q154
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_011528
Location1782702..1816475
NameDot/Icm
Functioneffector translocation
ClassificationType IVB; Type I
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentDotADotBDotCDotDIcmBIcmCIcmDIcmEIcmF
Number111111110
ComponentIcmGIcmHIcmJIcmKIcmLIcmMIcmNIcmOIcmP
Number101120111
ComponentIcmQIcmRIcmSIcmTIcmVIcmWIcmXLvgA
Number10111110

The information of T4SS components from NC_011528
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1CbuK_18361778939..1779034 [-], 96212219314hypothetical protein 
2CbuK_18371779138..1779293 [-], 156212219315hypothetical protein 
3CbuK_18381779326..1779604 [-], 279212219316hypothetical protein 
4CbuK_18401781108..1781242 [-], 135212219317hypothetical protein 
5CbuK_18411781401..1781724 [+], 324212219318hypothetical protein 
6CbuK_18431782550..1782687 [+], 138212219319hypothetical protein 
7CbuK_1844 (icmB)1782702..1785713 [-], 3012212219320IcmB  IcmB
8CbuK_1845 (icmJ)1785786..1786424 [-], 639212219321IcmJ  IcmJ
9CbuK_1846 (icmD)1786437..1786862 [-], 426212219322IcmD  IcmD
10CbuK_1847 (icmC)1786947..1787456 [-], 510212219323IcmC  IcmC
11CbuK_1848 (icmG)1787527..1788261 [-], 735212219324IcmG  IcmG
12CbuK_1849 (icmE)1788279..1791398 [-], 3120212219325IcmE  IcmE
13CbuK_1850 (icmK)1791385..1792440 [-], 1056212219326IcmK  IcmK
14CbuK_1851 (icmL.1)1792451..1793107 [-], 657212219327IcmL  IcmL
15CbuK_1852 (icmL.2)1793109..1793732 [-], 624212219328IcmL  IcmL
16CbuK_1853 (icmN)1793739..1794377 [-], 639212219329IcmN protein, OmpA family  IcmN
17CbuK_1854 (icmO)1794387..1796765 [-], 2379212219330IcmO  IcmO
18CbuK_1855 (icmP)1796741..1797904 [-], 1164212219331IcmP  IcmP
19CbuK_1856 (icmQ)1797905..1798621 [-], 717212219332IcmQ  IcmQ
20CbuK_18571798618..1798767 [-], 150212219333hypothetical protein 
21CbuK_18581798883..1799056 [+], 174212219334hypothetical protein 
22CbuK_18601800417..1800554 [+], 138212219335hypothetical protein 
23CbuK_18611800651..1801856 [-], 1206212219336ATPase 
24CbuK_18621801968..1803164 [-], 1197212219337hypothetical protein 
25CbuK_18631803256..1803384 [-], 129212219338hypothetical protein 
26CbuK_1865 (icmT)1804869..1805126 [+], 258212219339IcmT  IcmT
27CbuK_1866 (icmS)1805126..1805464 [+], 339212219340IcmS  IcmSaccesspry protein
28CbuK_1867 (dotD)1805621..1806130 [+], 510212219341DotD  DotD
29CbuK_1868 (dotC)1806133..1806957 [+], 825212219342DotC  DotC
30CbuK_1869 (dotB)1806995..1808113 [+], 1119212219343ATP-binding protein  DotB
31CbuK_18701808120..1809454 [+], 1335212219344glycine betaine transporter 
32CbuK_18711809620..1809892 [+], 273212219345hypothetical protein 
33CbuK_18721809889..1810983 [-], 1095212219346transposase 
34CbuK_18731811150..1811245 [-], 96212219347hypothetical protein 
35CbuK_1874 (dotA)1811300..1813744 [-], 2445212219348DotA  DotA
36CbuK_1875 (icmV)1813741..1814235 [-], 495212219349IcmV  IcmV
37CbuK_1876 (icmW)1814425..1814874 [+], 450212219350IcmW  IcmW
38CbuK_18771814871..1815329 [+], 459212219351hypothetical membrane-associated protein 
39CbuK_1878 (icmX)1815345..1816475 [+], 1131212219352IcmX  IcmX
40CbuK_18791816557..1816991 [+], 435212219353hypothetical protein 
41CbuK_18811818098..1818406 [-], 309212219354hypothetical protein 
42CbuK_18821818510..1819061 [-], 552212219355hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
CBUK_0083, CbuK_0120, CbuK_0121, CBUK_0201, CbuK_0228, CbuK_0231, CbuK_0236, CbuK_0241, AnkM, CBUK_0252, CbuK_0255, CbuK_0299, CBUK_0333, CbuK_0362, CBUK_0377, CBUK_0392, ElpA, CbuK_0427, CbuK_0462, CbuK_0466, CbuK_0491, CBUK_0540, CBUK_0601, CbuK_0608, CbuK_0611, CBUK_0643, AnkG, CbuK_0662, CbuK_0670, CbuK_0684, CBUK_0750, CBUK_0790, CbuK_0792, AnkH, CbuK_0900, CbuK_0942, CBUK_0945, CBUK_0972, CbuK_0989, CbuK_1050, CBUK_1062, AnkI, CbuK_1077, AnkI, CbuK_1093, CbuK_1119, CbuK_1177, CbuK_1236, CbuK_1237, CbuK_1239, CBUK_1248, CbuK_1285, CbuK_1313, AnkN, CBUK_1341, CbuK_1355, CbuK_1366, AnkF, CbuK_1448, CbuK_1450, CbuK_1480, CbuK_1498, CBUK_1593, CBUK_1619, CbuK_1636, CbuK_1660, CBUK_1672, CBUK_1673, CbuK_1685, CbuK_1690, CbuK_1751, CbuK_1769, CbuK_1772, CbuK_1785, CBUK_1793, CbuK_1800, CbuK_1805, CBUK_1806, CBUK_1823, CBUK_1834, CBUK_1862, CbuK_1872, AnkB, CbuK_1976, CbuK_1977, CBUK_1983, CbuK_1986, CbuK_2014, CBUK_2058, CBUK_2064, CBUK_2067, CbuK_2099, CbuK_2102, CbuK_2106, CBUK_2107, CbuK_2116, CBUK_2121, CbuK_2125, CbuK_2131

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1CbuK_0083 78542..79408 [-], 867212217764gluconolactonaseexperimental  CBUK_0083
2CbuK_0120 112895..113314 [+], 420212217798hypothetical protein  CbuK_0120
3CbuK_0121 112743..112904 [-], 162212217799hypothetical protein  CbuK_0121
4CbuK_0201 180666..181478 [-], 813212217874hypothetical cytosolic proteinexperimental  CBUK_0201
5CbuK_0228 205137..206231 [+], 1095212217899transposase  CbuK_0228
6CbuK_0231 208007..208930 [-], 924212217901hypothetical protein  CbuK_0231
7CbuK_0236 213867..214484 [+], 618212217906alpha/beta hydrolase  CbuK_0236
8CbuK_0241 218059..219153 [+], 1095212217911transposase  CbuK_0241
9CbuK_0251 229100..229225 [+], 126212217920hypothetical protein  AnkM
10CbuK_0252 229235..229831 [+], 597212217921hypothetical proteinexperimental  CBUK_0252
11CbuK_0255 231617..232879 [-], 1263212217924hypothetical protein  CbuK_0255
12CbuK_0299 275068..276132 [-], 1065212217965transposase  CbuK_0299
13CbuK_0333 304832..305266 [+], 435212217994hypothetical cytosolic proteinexperimental  CBUK_0333
14CbuK_0362 328544..329284 [+], 741212218018serine/threonine protein kinase domain protein  CbuK_0362
15CbuK_0377 343973..344239 [+], 267212218027hypothetical proteinexperimental  CBUK_0377
16CbuK_0392 361241..361636 [-], 396212218040ankyrin repeat proteinexperimental  CBUK_0392
17CbuK_0401 369951..371207 [+], 1257212218047hypothetical membrane spanning proteinexperimental  ElpA
18CbuK_0427 395518..396612 [-], 1095212218068transposase  CbuK_0427
19CbuK_0462 417633..418853 [+], 1221212218102trans-2-enoyl-CoA reductase  CbuK_0462
20CbuK_0466 421709..422803 [+], 1095212218106transposase  CbuK_0466
21CbuK_0491 (algC)441393..442808 [+], 1416212218130phosphoglucomutase  CbuK_0491
22CbuK_0540 492637..493425 [+], 789212218176hypothetical proteinexperimental  CBUK_0540
23CbuK_0601 547248..547709 [+], 462212218227hypothetical proteinexperimental  CBUK_0601
24CbuK_0608 553801..554895 [-], 1095212218234transposase  CbuK_0608
25CbuK_0611 556103..557197 [-], 1095212218237transposase  CbuK_0611
26CbuK_0643 588557..589027 [+], 471212218264PhnBexperimental  CBUK_0643
27CbuK_0651 593744..594412 [+], 669212218271ankyrin repeat proteinexperimental  AnkGinteraction
28CbuK_0662 610962..611993 [-], 1032212218279hypothetical protein  CbuK_0662
29CbuK_0670 (rimI)616641..617078 [+], 438212218285ribosomal-protein-S18-alanine acetyltransferase  CbuK_0670
30CbuK_0684 633253..635106 [+], 1854212218298hypothetical membrane spanning protein  CbuK_0684
31CbuK_0750 704375..705541 [+], 1167212218363hypothetical cytosolic proteinexperimental  CBUK_0750
32CbuK_0790 743664..745934 [+], 2271212218399hypothetical membrane-associated proteinexperimental  CBUK_0790
33CbuK_0792 747798..747911 [+], 114212218400hypothetical protein  CbuK_0792
34CbuK_0816 771548..771853 [-], 306212218417hypothetical protein  AnkH
35CbuK_0900 845324..846775 [-], 1452212218485hypothetical protein  CbuK_0900
36CbuK_0942 888464..889558 [-], 1095212218520transposase  CbuK_0942
37CbuK_0945 890916..891512 [-], 597212218523hypothetical proteinexperimental  CBUK_0945
38CbuK_0972 916033..917082 [+], 1050212218546hypothetical cytosolic proteinexperimental  CBUK_0972
39CbuK_0989 938007..939101 [+], 1095212218560transposase  CbuK_0989
40CbuK_1050 1001574..1002668 [+], 1095212218611transposase  CbuK_1050
41CbuK_1062 1015134..1015670 [+], 537212218623hypothetical cytosolic proteinexperimental  CBUK_1062
42CbuK_1074 1025929..1026033 [+], 105212218634hypothetical cytosolic protein  AnkI
43CbuK_1077 1028446..1029885 [+], 1440212218636hypothetical membrane spanning protein  CbuK_1077
44CbuK_1081 1032089..1032682 [-], 594212218638hypothetical membrane spanning protein  AnkI
45CbuK_1093 1052098..1053192 [-], 1095212218649transposase  CbuK_1093
46CbuK_1119 1077142..1078236 [+], 1095212218672transposase  CbuK_1119
47CbuK_1177 1132076..1132720 [-], 645212218726hypothetical cytosolic protein  CbuK_1177
48CbuK_1236 1195036..1195170 [+], 135212218779hypothetical protein  CbuK_1236
49CbuK_1237 1195463..1197442 [-], 1980212218780protein kinase family  CbuK_1237
50CbuK_1239 1197894..1198988 [-], 1095212218782transposase  CbuK_1239
51CbuK_1248 1205186..1205359 [-], 174212218790hypothetical proteinexperimental  CBUK_1248
52CbuK_1285 1243044..1244138 [-], 1095212218825transposase  CbuK_1285
53CbuK_1313 1279295..1280389 [+], 1095212218852transposase  CbuK_1313
54CbuK_1330 1297578..1299170 [-], 1593212218865ankyrin repeat proteinexperimental  AnkN
55CbuK_1341 1306070..1307092 [-], 1023212218876fructose-1,6-bisphosphataseexperimental  CBUK_1341
56CbuK_1355 1318812..1319906 [-], 1095212218890transposase  CbuK_1355
57CbuK_1366 1327283..1328377 [-], 1095212218901transposase  CbuK_1366
58CbuK_1411 1369136..1369690 [+], 555212218943ankyrin repeat proteinexperimental  AnkF
59CbuK_1448 1400983..1402077 [+], 1095212218975transposase  CbuK_1448
60CbuK_1450 1402461..1403201 [+], 741212218977hypothetical protein  CbuK_1450
61CbuK_1480 1431571..1432404 [-], 834212219004hypothetical protein  CbuK_1480
62CbuK_1498 1446194..1446646 [-], 45321221902217 kDa common-antigen  CbuK_1498
63CbuK_1593 1539725..1540804 [-], 1080212219104hypothetical proteinexperimental  CBUK_1593
64CbuK_1619 1561381..1562142 [+], 762212219128coenzyme PQQ synthesis protein Cexperimental  CBUK_1619
65CbuK_1636 1581960..1583054 [+], 1095212219144transposase  CbuK_1636
66CbuK_1660 1602695..1602796 [+], 102212219162hypothetical protein  CbuK_1660
67CbuK_1672 1614361..1615551 [-], 1191212219172hypothetical proteinexperimental  CBUK_1672
68CbuK_1673 1615690..1615824 [+], 135212219173hypothetical proteinexperimental  CBUK_1673
69CbuK_1685 1629280..1631211 [-], 1932212219181tetratricopeptide repeat family protein  CbuK_1685
70CbuK_1690 1634645..1636915 [-], 2271212219185hypothetical membrane-associated protein  CbuK_1690
71CbuK_1751 1690703..1691344 [+], 642212219241hypothetical protein  CbuK_1751
72CbuK_1769 1711912..1712505 [-], 594212219256hypothetical protein  CbuK_1769
73CbuK_1772 1712945..1714039 [-], 1095212219259transposase  CbuK_1772
74CbuK_1785 1727237..1728940 [+], 1704212219271hypothetical membrane spanning protein  CbuK_1785
75CbuK_1793 1736020..1736754 [+], 735212219278hypothetical proteinexperimental  CBUK_1793
76CbuK_1800 1741251..1742270 [-], 1020212219284transposase  CbuK_1800
77CbuK_1805 1745209..1746303 [-], 1095212219289transposase  CbuK_1805
78CbuK_1806 1748573..1748692 [-], 120212219290hypothetical proteinexperimental  CBUK_1806
79CbuK_1823 1762185..1762634 [+], 450212219305GatB/Yqey domain proteinexperimental  CBUK_1823
80CbuK_1834 1775346..1775744 [-], 399212219313hypothetical proteinexperimental  CBUK_1834
81CbuK_1862 1801968..1803164 [-], 1197212219337hypothetical proteinexperimental  CBUK_1862
82CbuK_1872 1809889..1810983 [-], 1095212219346transposase  CbuK_1872
83CbuK_1907 1842210..1843289 [-], 1080212219375ankyrin repeat proteinexperimental  AnkB
84CbuK_1976 1902270..1903259 [-], 990212219441hypothetical transcriptional regulatory proteinexperimental  CbuK_1976
85CbuK_1977 1903435..1904226 [-], 792212219442hypothetical membrane spanning protein  CbuK_1977
86CbuK_1983 1912280..1912393 [+], 114212219447hypothetical proteinexperimental  CBUK_1983
87CbuK_1986 1914019..1916151 [-], 2133212219449hypothetical protein  CbuK_1986
88CbuK_2014 1940660..1942654 [+], 1995212219472hypothetical cytosolic protein  CbuK_2014
89CbuK_2058 1976039..1977226 [-], 1188212219515hypothetical proteinexperimental  CBUK_2058
90CbuK_2064 1982973..1984070 [-], 1098212219521hypothetical proteinexperimental  CBUK_2064
91CbuK_2067 1986459..1987055 [+], 597212219524hypothetical cytosolic proteinexperimental  CBUK_2067
92CbuK_2099 2021555..2022451 [-], 897212219549hypothetical protein  CbuK_2099
93CbuK_2102 2025759..2026427 [-], 669212219552hypothetical protein  CbuK_2102
94CbuK_2106 2031243..2031380 [-], 138212219555hypothetical protein  CbuK_2106
95CbuK_2107 2031738..2032784 [+], 1047212219556hypothetical cytosolic proteinexperimental  CBUK_2107
96CbuK_2116 2039046..2040140 [-], 1095212219565transposase  CbuK_2116
97CbuK_2121 2043330..2043647 [+], 318212219570hypothetical proteinexperimental  CBUK_2121
98CbuK_2125 2045954..2047000 [-], 1047212219573Fic family protein  CbuK_2125
99CbuK_2131 2051489..2052583 [+], 1095212219579transposase  CbuK_2131

interaction This effector contains information of interaction.

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Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1IcmS (chaperone) (212219340)NDAnkB, AnkH, AnkI, AnkJ, AnkMThe chaperone IcmS is essential for translocation into the host cytosol.(1) PubMed: 19411324
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1AnkG (212218271)gC1qR (p32)human, murineAnkG interacts with gC1qR(p32) to regulate an anti-apoptotic pathway which is required for adaptation to mammalian hosts of Coxiella.(1) PubMed: 20944063insolico

insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Luhrmann A; Nogueira CV; Carey KL; Roy CR (2010). Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. Proc Natl Acad Sci U S A. 107(44):18997-9001. [PudMed:20944063] experimental
(1) McDonough JA; Newton HJ; Roy CR (2012). Coxiella burnetii Secretion Systems. Adv Exp Med Biol. 984:171-97. [PudMed:22711632]
(2) Chen C; Banga S; Mertens K; Weber MM; Gorbaslieva I; Tan Y; Luo ZQ; Samuel JE (2010). Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii. Proc Natl Acad Sci U S A. 107(50):21755-60. [PudMed:21098666] experimental
(3) Voth DE; Howe D; Beare PA; Vogel JP; Unsworth N; Samuel JE; Heinzen RA (2009). The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol. 191(13):4232-42. [PudMed:19411324] experimental
(4) Pan X; Luhrmann A; Satoh A; Laskowski-Arce MA; Roy CR (2008). Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Science. 320(5883):1651-4. [PudMed:18566289] experimental in_silico
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU