Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU46_RS05555 Genome accession   NZ_CP155941
Coordinates   1300175..1301908 (+) Length   577 a.a.
NCBI ID   WP_011038212.1    Uniprot ID   Q8P673
Organism   Xanthomonas campestris pv. campestris strain IPV-CT 62.4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1295175..1306908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU46_RS05530 (ABFU46_05530) coaE 1296066..1296689 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  ABFU46_RS05535 (ABFU46_05535) - 1296703..1297566 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU46_RS05540 (ABFU46_05540) pilC 1297573..1298829 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  ABFU46_RS05545 (ABFU46_05545) pilE 1299156..1299596 (+) 441 WP_011038214.1 pilin Machinery gene
  ABFU46_RS05550 (ABFU46_05550) - 1299709..1300110 (+) 402 WP_194734302.1 pilin -
  ABFU46_RS05555 (ABFU46_05555) pilB 1300175..1301908 (+) 1734 WP_011038212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU46_RS05560 (ABFU46_05560) pilR 1302325..1303719 (-) 1395 WP_012437607.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU46_RS05565 (ABFU46_05565) - 1303928..1305538 (-) 1611 WP_012437608.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62785.06 Da        Isoelectric Point: 5.5903

>NTDB_id=999966 ABFU46_RS05555 WP_011038212.1 1300175..1301908(+) (pilB) [Xanthomonas campestris pv. campestris strain IPV-CT 62.4]
MSVVLTANLVGITGIARRLVQDGALEEAVARGAMDQAALAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=999966 ABFU46_RS05555 WP_011038212.1 1300175..1301908(+) (pilB) [Xanthomonas campestris pv. campestris strain IPV-CT 62.4]
ATGAGCGTTGTGCTAACGGCTAATCTTGTTGGAATCACGGGCATTGCTCGACGTCTTGTGCAAGACGGTGCGCTCGAAGA
GGCGGTTGCTCGTGGTGCGATGGATCAGGCAGCACTCGCCAAAGTGCCTTTACCCCAGTGGTTTGCTGAAAAAAAGCTAG
TCTCTGCTGCGCAGCTCGCCTCTGCCAATGCAGTTGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAGCATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACGCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTAGACGAAGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGCTCGGC
GACGACGATGATGAGATGGGGGATCTGGACGTCTCTACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGCTATGAGGCGGACCAACAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAACATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCACCGTACAACATCA
CCTCCTCGGTGACCTTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTAATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTCACCAGCCTCGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.44

0.553
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

51.937

98.44

0.511
  pilF Neisseria gonorrhoeae MS11

48.415

98.44

0.477
  pilB Vibrio cholerae strain A1552

49.002

95.494

0.468
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment