Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ABC799_RS01140 Genome accession   NZ_CP155534
Coordinates   219977..220993 (+) Length   338 a.a.
NCBI ID   WP_078130737.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain DCC1476     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214977..225993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC799_RS01120 (ABC799_01120) nagA 216124..217275 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ABC799_RS01125 (ABC799_01125) - 217438..218496 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ABC799_RS01130 (ABC799_01130) - 218647..219012 (+) 366 WP_000286415.1 DUF1033 family protein -
  ABC799_RS01135 (ABC799_01135) comGA/cglA/cilD 219088..220029 (+) 942 WP_000249536.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC799_RS01140 (ABC799_01140) comGB/cglB 219977..220993 (+) 1017 WP_078130737.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC799_RS01145 (ABC799_01145) comGC/cglC 220995..221318 (+) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  ABC799_RS01150 (ABC799_01150) comGD/cglD 221281..221715 (+) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC799_RS01155 (ABC799_01155) comGE/cglE 221678..221980 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC799_RS01160 (ABC799_01160) comGF/cglF 221943..222404 (+) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC799_RS01165 (ABC799_01165) comGG/cglG 222382..222774 (+) 393 WP_050202681.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC799_RS01170 (ABC799_01170) - 222930..223522 (+) 593 Protein_214 methyltransferase domain-containing protein -
  ABC799_RS01175 (ABC799_01175) comYH 223583..224536 (+) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  ABC799_RS01180 (ABC799_01180) - 224587..225777 (+) 1191 WP_000167765.1 acetate kinase -
  ABC799_RS01185 (ABC799_01185) - 225778..225909 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38523.66 Da        Isoelectric Point: 9.8240

>NTDB_id=997233 ABC799_RS01140 WP_078130737.1 219977..220993(+) (comGB/cglB) [Streptococcus pneumoniae strain DCC1476]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=997233 ABC799_RS01140 WP_078130737.1 219977..220993(+) (comGB/cglB) [Streptococcus pneumoniae strain DCC1476]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGAAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGTAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment