Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQB42_RS00140 Genome accession   NZ_LR129841
Coordinates   25735..27096 (+) Length   453 a.a.
NCBI ID   WP_073176637.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 947 isolate 947     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20735..32096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB42_RS00105 - 21105..21889 (-) 785 Protein_15 transposase -
  EQB42_RS00110 comW 22154..22390 (+) 237 WP_000939545.1 sigma(X)-activator ComW Regulator
  EQB42_RS00115 - 22621..23907 (+) 1287 WP_001813655.1 adenylosuccinate synthase -
  EQB42_RS00120 tadA 24108..24575 (+) 468 WP_000291872.1 tRNA adenosine(34) deaminase TadA -
  EQB42_RS00130 - 24761..25204 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EQB42_RS00135 - 25206..25721 (+) 516 WP_001828232.1 histidine phosphatase family protein -
  EQB42_RS00140 radA 25735..27096 (+) 1362 WP_073176637.1 DNA repair protein RadA Machinery gene
  EQB42_RS00145 - 27169..27666 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  EQB42_RS00150 - 27687..27974 (+) 288 Protein_23 PrsW family intramembrane metalloprotease -
  EQB42_RS00155 - 28028..29379 (+) 1352 WP_088778796.1 IS3 family transposase -
  EQB42_RS00160 - 29409..29945 (+) 537 Protein_25 PrsW family glutamic-type intramembrane protease -
  EQB42_RS00165 - 30090..31058 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49446.98 Da        Isoelectric Point: 6.5137

>NTDB_id=993729 EQB42_RS00140 WP_073176637.1 25735..27096(+) (radA) [Streptococcus pneumoniae strain 947 isolate 947]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=993729 EQB42_RS00140 WP_073176637.1 25735..27096(+) (radA) [Streptococcus pneumoniae strain 947 isolate 947]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATATCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTTATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCAACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.355

100

0.634

Multiple sequence alignment