Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KKKWG1_RS00320 Genome accession   NZ_LN869922
Coordinates   60764..61192 (-) Length   142 a.a.
NCBI ID   WP_019390096.1    Uniprot ID   B6SE74
Organism   Kingella kingae strain KWG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 55764..66192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKKWG1_RS00290 (KKKWG1_0063) - 56168..57013 (+) 846 WP_019390091.1 mechanosensitive ion channel family protein -
  KKKWG1_RS11060 - 57275..57433 (+) 159 WP_019390092.1 hypothetical protein -
  KKKWG1_RS00295 (KKKWG1_0064) - 57436..58161 (+) 726 WP_019390093.1 metal ABC transporter ATP-binding protein -
  KKKWG1_RS00300 (KKKWG1_0065) - 58210..58602 (+) 393 WP_003791724.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  KKKWG1_RS00305 (KKKWG1_0066) - 58640..59530 (+) 891 WP_019390094.1 metal ABC transporter permease -
  KKKWG1_RS00315 (KKKWG1_0067) - 59976..60752 (-) 777 WP_019390095.1 fimb protein -
  KKKWG1_RS00320 (KKKWG1_0068) pilE 60764..61192 (-) 429 WP_019390096.1 pilin Machinery gene
  KKKWG1_RS00325 (KKKWG1_0069) pilE 61339..61809 (-) 471 WP_019390097.1 pilin Machinery gene
  KKKWG1_RS00330 (KKKWG1_0070) recJ 62169..63881 (+) 1713 WP_060536894.1 single-stranded-DNA-specific exonuclease RecJ -
  KKKWG1_RS00335 (KKKWG1_0072) - 63993..64727 (+) 735 WP_003791735.1 histidine phosphatase family protein -
  KKKWG1_RS00340 (KKKWG1_0073) - 64767..65312 (+) 546 WP_019390101.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14865.01 Da        Isoelectric Point: 7.8822

>NTDB_id=992284 KKKWG1_RS00320 WP_019390096.1 60764..61192(-) (pilE) [Kingella kingae strain KWG1]
METKMQGFTLIELMIVIAIVGILAALALPTYQDFSVRSRVAEGLSLAGGAKTSVAEYYASHNQFPANNVTAGLATANQIK
GSSVHSLTVTDGVITVVFNNKVAAAGKNSLVLTPSVAKEGSITWDCHGGTLDGRLRPSECRN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=992284 KKKWG1_RS00320 WP_019390096.1 60764..61192(-) (pilE) [Kingella kingae strain KWG1]
ATGGAAACTAAAATGCAAGGTTTTACCTTAATTGAATTGATGATTGTTATTGCTATTGTGGGTATTTTAGCTGCGCTGGC
ATTGCCAACGTATCAGGATTTTTCAGTACGCTCGCGTGTGGCAGAAGGATTGAGTTTGGCTGGTGGTGCAAAAACATCGG
TGGCTGAATATTATGCTTCGCATAACCAATTTCCAGCCAATAATGTAACGGCAGGTTTGGCTACGGCCAACCAAATTAAG
GGCAGTTCGGTGCATTCGCTTACGGTAACAGATGGTGTGATTACGGTGGTATTTAATAATAAAGTGGCGGCAGCAGGTAA
AAATTCGCTGGTTTTAACGCCTAGTGTGGCGAAAGAAGGCTCTATTACTTGGGATTGCCACGGTGGTACATTGGACGGCA
GATTGCGTCCATCGGAATGCCGTAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6SE74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.465
  pilE Neisseria gonorrhoeae strain FA1090

39.877

100

0.458
  pilA/pilA1 Eikenella corrodens VA1

41.935

100

0.458
  pilA2 Legionella pneumophila str. Paris

45.324

97.887

0.444
  comP Acinetobacter baylyi ADP1

40.26

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

44.604

97.887

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.806

100

0.373