Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NYE28_RS08960 Genome accession   NZ_CP152013
Coordinates   1838067..1838501 (+) Length   144 a.a.
NCBI ID   WP_160223458.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0443     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1833067..1843501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE28_RS08955 (NYE28_08955) - 1836567..1837778 (-) 1212 WP_052827455.1 cytochrome P450 -
  NYE28_RS08960 (NYE28_08960) nucA/comI 1838067..1838501 (+) 435 WP_160223458.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NYE28_RS08965 (NYE28_08965) - 1838561..1839316 (+) 756 WP_003154084.1 YoaK family protein -
  NYE28_RS08970 (NYE28_08970) - 1839350..1839712 (-) 363 WP_007410383.1 hypothetical protein -
  NYE28_RS08975 (NYE28_08975) - 1839905..1841233 (-) 1329 WP_012117603.1 S8 family peptidase -
  NYE28_RS08980 (NYE28_08980) - 1841412..1841645 (+) 234 WP_015239897.1 hypothetical protein -
  NYE28_RS08985 (NYE28_08985) - 1841901..1842608 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  NYE28_RS08990 (NYE28_08990) - 1842668..1843120 (+) 453 WP_014305039.1 OsmC family protein -
  NYE28_RS08995 (NYE28_08995) - 1843134..1843487 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15601.52 Da        Isoelectric Point: 7.2418

>NTDB_id=984899 NYE28_RS08960 WP_160223458.1 1838067..1838501(+) (nucA/comI) [Bacillus sp. FSL R5-0443]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRNGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=984899 NYE28_RS08960 WP_160223458.1 1838067..1838501(+) (nucA/comI) [Bacillus sp. FSL R5-0443]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGAACGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment