Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MKY18_RS12310 Genome accession   NZ_CP152010
Coordinates   2429920..2432244 (-) Length   774 a.a.
NCBI ID   WP_185107048.1    Uniprot ID   -
Organism   Bacillus sp. FSL R7-0672     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2424920..2437244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY18_RS12295 (MKY18_12295) dtd 2426569..2427012 (-) 444 WP_044140029.1 D-aminoacyl-tRNA deacylase -
  MKY18_RS12300 (MKY18_12300) - 2427031..2429241 (-) 2211 WP_044140028.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MKY18_RS12305 (MKY18_12305) - 2429384..2429896 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  MKY18_RS12310 (MKY18_12310) recJ 2429920..2432244 (-) 2325 WP_185107048.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MKY18_RS12315 (MKY18_12315) - 2432326..2432679 (-) 354 WP_044140026.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MKY18_RS12320 (MKY18_12320) - 2432782..2433271 (-) 490 Protein_2385 cation:proton antiporter regulatory subunit -
  MKY18_RS12325 (MKY18_12325) secDF 2433458..2435686 (-) 2229 WP_003216337.1 protein translocase subunit SecDF -
  MKY18_RS12330 (MKY18_12330) - 2435728..2436024 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87432.21 Da        Isoelectric Point: 4.9922

>NTDB_id=984693 MKY18_RS12310 WP_185107048.1 2429920..2432244(-) (recJ) [Bacillus sp. FSL R7-0672]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDKFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRANDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDVRLEEIRTIGTNQNHIKMSLKDEDKLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVKIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSAFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=984693 MKY18_RS12310 WP_185107048.1 2429920..2432244(-) (recJ) [Bacillus sp. FSL R7-0672]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTTTTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCATCATGAACCAAGTGAGGAGCTTCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGATGTGAGTATCCGTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCTCTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTAGGCAGTCGAATAGACCGGGGCTGAAGGCGTTATTAAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAACAGGCCGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCAATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAAATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGCGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGGGCGAATGATGT
TCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTTG
ATGCTGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGTGAGACTTGAAGAAATCCGAACAATTGGAACAAATCAGAATCATATTAAAAT
GTCTCTGAAAGATGAAGATAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAGCTCATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACCATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAAAATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTCGTCTTTGCAGATGTTCCTGCAGATGAACACTTGCTAAGGCAGCTGTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCGCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCTCTCAAGGAACAAGGAATGGAGCTTGCGAAACATA
GGGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTTGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACAGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(309-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

66.924

100

0.672

Multiple sequence alignment