Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAFF32_RS19370 Genome accession   NZ_CP151802
Coordinates   4190089..4191147 (-) Length   352 a.a.
NCBI ID   WP_216964071.1    Uniprot ID   -
Organism   Lysobacter sp. FW306-1B-D06B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4185089..4196147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF32_RS19350 (AAFF32_19350) - 4186120..4188054 (-) 1935 WP_216964064.1 type IV pilus secretin PilQ family protein -
  AAFF32_RS19355 (AAFF32_19355) - 4188074..4188628 (-) 555 WP_216964066.1 pilus assembly protein PilP -
  AAFF32_RS19360 (AAFF32_19360) pilO 4188630..4189304 (-) 675 WP_342316058.1 type 4a pilus biogenesis protein PilO -
  AAFF32_RS19365 (AAFF32_19365) - 4189301..4190089 (-) 789 WP_342316059.1 PilN domain-containing protein -
  AAFF32_RS19370 (AAFF32_19370) pilM 4190089..4191147 (-) 1059 WP_216964071.1 pilus assembly protein PilM Machinery gene
  AAFF32_RS19375 (AAFF32_19375) - 4191394..4193886 (+) 2493 WP_216964072.1 penicillin-binding protein 1A -
  AAFF32_RS19380 (AAFF32_19380) - 4194109..4194777 (+) 669 WP_216964074.1 hypothetical protein -
  AAFF32_RS19385 (AAFF32_19385) - 4194781..4195392 (-) 612 WP_342316060.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37536.88 Da        Isoelectric Point: 4.4909

>NTDB_id=980942 AAFF32_RS19370 WP_216964071.1 4190089..4191147(-) (pilM) [Lysobacter sp. FW306-1B-D06B]
MGIVTKSQPAVVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKSIVEVEAVGEAIRRAVARSGTRAKHAAAA
VAGSSVITKVIPMPGDLDDDDLESQVELEAVNYVPYPIEEVNHDFEVLGPMPGSPDMLQVLLAASRSENVEVRASALEIG
GLTPKVMDVEAFALENAFALVADQLGGPRDGLVALVDSGATMTTLNVLRNGRSLYAREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEAEVLEPFKEAMVQQISRLLQFFYAGSEFNRVDQIVLAGGCASIAGIAEMVEEQLGVPTTIANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=980942 AAFF32_RS19370 WP_216964071.1 4190089..4191147(-) (pilM) [Lysobacter sp. FW306-1B-D06B]
GTGGGGATCGTCACAAAAAGCCAGCCGGCAGTCGTCGGCGTGGACATCAGTTCGACTGCCGTAAAGCTGTTGCAGCTCTC
CCGGGCCGGCAACCGCTACCGCGTCGAGCATTACGCGGTCGAACCGCTGCCGCCGAACGCCGTCGTCGAGAAGAGCATCG
TCGAGGTCGAGGCCGTGGGCGAGGCGATCCGGCGCGCCGTTGCGCGGTCGGGCACGCGGGCCAAGCACGCCGCCGCCGCC
GTGGCCGGCTCGTCGGTCATCACCAAGGTCATCCCGATGCCGGGCGACCTGGATGACGACGACCTGGAGTCGCAGGTCGA
GCTGGAGGCGGTGAACTACGTCCCGTACCCCATCGAGGAAGTCAATCACGATTTCGAGGTGCTGGGCCCGATGCCCGGCA
GCCCGGACATGCTGCAGGTCCTGCTCGCCGCCTCACGTTCGGAGAACGTGGAAGTGCGCGCCTCGGCGCTGGAGATCGGC
GGCCTCACCCCGAAGGTGATGGACGTTGAAGCCTTCGCGCTGGAGAACGCCTTCGCGCTGGTCGCCGACCAGCTCGGCGG
TCCGCGCGATGGCCTGGTCGCCCTGGTCGATTCCGGCGCGACGATGACCACGCTCAACGTGCTTCGCAACGGCCGCAGCC
TGTACGCCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTCACCGACGAAGTCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGTCAGGGCGGACTGCCGGAGAGCTACGAAGCCGAAGTCCTGGAGCCCTTCAAGGAGGCGATGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAGTTCAACCGCGTCGACCAGATCGTGCTGGCCGGCG
GTTGCGCCTCCATCGCCGGCATCGCCGAGATGGTCGAGGAGCAGCTCGGCGTGCCGACCACCATCGCCAACCCGCTCGCC
CACATGACCCTCGGGCCGCGCGTGCAGGCACATGCTTTGGCCCAGGACGCACCGGCGCTGATGATCGCCTGCGGTCTCGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.716

99.148

0.503
  comM Acinetobacter nosocomialis M2

50.432

98.58

0.497
  comM Acinetobacter baylyi ADP1

49.856

98.58

0.491
  pilM Legionella pneumophila strain ERS1305867

45.64

97.727

0.446

Multiple sequence alignment