Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   R8563_RS10645 Genome accession   NZ_AP026917
Coordinates   2047269..2048567 (-) Length   432 a.a.
NCBI ID   WP_000867612.1    Uniprot ID   A0A0E9GXK4
Organism   Streptococcus pneumoniae strain PZ900700007     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2042269..2053567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8563_RS10625 (PC0007_20440) rplI 2043424..2043876 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  R8563_RS10630 (PC0007_20450) - 2043873..2045846 (-) 1974 WP_000715108.1 DHH family phosphoesterase -
  R8563_RS10635 (PC0007_20460) hpf 2045982..2046530 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R8563_RS10640 (PC0007_20470) comFC/cflB 2046610..2047272 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  R8563_RS10645 (PC0007_20480) comFA/cflA 2047269..2048567 (-) 1299 WP_000867612.1 DEAD/DEAH box helicase Machinery gene
  R8563_RS10650 (PC0007_20490) - 2048623..2049258 (+) 636 WP_000395739.1 YigZ family protein -
  R8563_RS10655 (PC0007_20500) cysK 2049543..2050463 (+) 921 WP_000029867.1 cysteine synthase A -
  R8563_RS10660 - 2050702..2051076 (-) 375 Protein_2059 IS66 family transposase -
  R8563_RS10665 (PC0007_20520) - 2051049..2051336 (+) 288 Protein_2060 transposase -
  R8563_RS10670 (PC0007_20530) tnpB 2051477..2051692 (-) 216 WP_000376912.1 IS66 family insertion sequence element accessory protein TnpB -
  R8563_RS10675 (PC0007_20540) tsf 2052046..2053086 (-) 1041 WP_000808062.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49563.48 Da        Isoelectric Point: 8.4382

>NTDB_id=97762 R8563_RS10645 WP_000867612.1 2047269..2048567(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700007]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=97762 R8563_RS10645 WP_000867612.1 2047269..2048567(-) (comFA/cflA) [Streptococcus pneumoniae strain PZ900700007]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E9GXK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment