Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLFAG_RS29495 Genome accession   NZ_CP177247
Coordinates   6300387..6301010 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain WYT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6295387..6306010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFAG_RS29475 (ACLFAG_29470) aroB 6295982..6297088 (-) 1107 WP_019726985.1 3-dehydroquinate synthase -
  ACLFAG_RS29480 (ACLFAG_29475) aroK 6297138..6297656 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACLFAG_RS29485 (ACLFAG_29480) pilQ 6297668..6299812 (-) 2145 WP_019726987.1 type 4a pilus secretin PilQ Machinery gene
  ACLFAG_RS29490 (ACLFAG_29485) comL/pilP 6299866..6300390 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACLFAG_RS29495 (ACLFAG_29490) pilO 6300387..6301010 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLFAG_RS29500 (ACLFAG_29495) pilN 6301007..6301603 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACLFAG_RS29505 (ACLFAG_29500) pilM 6301603..6302667 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACLFAG_RS29510 (ACLFAG_29505) - 6302852..6305323 (+) 2472 WP_019726988.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=970181 ACLFAG_RS29495 WP_003103268.1 6300387..6301010(-) (pilO) [Pseudomonas aeruginosa strain WYT]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=970181 ACLFAG_RS29495 WP_003103268.1 6300387..6301010(-) (pilO) [Pseudomonas aeruginosa strain WYT]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401