Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   J215_RS25955 Genome accession   NZ_CP176783
Coordinates   5531652..5532125 (-) Length   157 a.a.
NCBI ID   WP_015503553.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain J215     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5526652..5537125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J215_RS25935 (J215_005075) ampD 5527393..5527959 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J215_RS25940 (J215_005076) - 5528110..5530365 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  J215_RS25945 (J215_005077) nadC 5530651..5531499 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J215_RS25955 pilE 5531652..5532125 (-) 474 WP_015503553.1 pilin Machinery gene
  J215_RS25960 (J215_005078) pilB 5532356..5534056 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J215_RS25965 (J215_005079) pilC 5534060..5535277 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  J215_RS25970 (J215_005080) pilD 5535280..5536152 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  J215_RS25975 (J215_005081) coaE 5536149..5536760 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  J215_RS25980 (J215_005082) yacG 5536757..5536957 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16090.45 Da        Isoelectric Point: 8.4684

>NTDB_id=968852 J215_RS25955 WP_015503553.1 5531652..5532125(-) (pilE) [Pseudomonas aeruginosa strain J215]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=968852 J215_RS25955 WP_015503553.1 5531652..5532125(-) (pilE) [Pseudomonas aeruginosa strain J215]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459
  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

41.975

100

0.433
  pilA2 Legionella pneumophila str. Paris

45.578

93.631

0.427
  pilA2 Legionella pneumophila strain ERS1305867

44.218

93.631

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

39.394

100

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395