Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | NSU05_RS12905 | Genome accession | NZ_CP150177 |
| Coordinates | 2521486..2521896 (-) | Length | 136 a.a. |
| NCBI ID | WP_038429332.1 | Uniprot ID | - |
| Organism | Bacillus sp. EU52 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2516851..2546038 | 2521486..2521896 | within | 0 |
Gene organization within MGE regions
Location: 2516851..2546038
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NSU05_RS12880 (NSU05_12880) | - | 2517018..2517749 (-) | 732 | WP_015714294.1 | SGNH/GDSL hydrolase family protein | - |
| NSU05_RS12885 (NSU05_12885) | cwlH | 2518002..2518754 (-) | 753 | WP_038429329.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| NSU05_RS12890 (NSU05_12890) | - | 2518941..2519567 (+) | 627 | WP_038429330.1 | TVP38/TMEM64 family protein | - |
| NSU05_RS12895 (NSU05_12895) | gnd | 2519586..2520479 (-) | 894 | WP_038429331.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| NSU05_RS12900 (NSU05_12900) | - | 2520731..2521453 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| NSU05_RS12905 (NSU05_12905) | nucA/comI | 2521486..2521896 (-) | 411 | WP_038429332.1 | sporulation-specific Dnase NucB | Machinery gene |
| NSU05_RS12910 (NSU05_12910) | sigK | 2522092..2522820 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| NSU05_RS12915 (NSU05_12915) | - | 2522820..2522912 (+) | 93 | Protein_2494 | hypothetical protein | - |
| NSU05_RS12920 (NSU05_12920) | - | 2522895..2523035 (-) | 141 | Protein_2495 | recombinase | - |
| NSU05_RS12925 (NSU05_12925) | - | 2523580..2524104 (-) | 525 | WP_038429334.1 | NAD(P)H-dependent oxidoreductase | - |
| NSU05_RS12930 (NSU05_12930) | - | 2524352..2524826 (-) | 475 | Protein_2497 | HXXEE domain-containing protein | - |
| NSU05_RS12935 (NSU05_12935) | - | 2525330..2526709 (-) | 1380 | WP_038429335.1 | aspartate aminotransferase family protein | - |
| NSU05_RS12940 (NSU05_12940) | - | 2526926..2527792 (+) | 867 | WP_038429336.1 | MurR/RpiR family transcriptional regulator | - |
| NSU05_RS12945 (NSU05_12945) | - | 2528526..2528867 (-) | 342 | WP_038429337.1 | MmcQ/YjbR family DNA-binding protein | - |
| NSU05_RS12950 (NSU05_12950) | - | 2528893..2530218 (-) | 1326 | WP_038429338.1 | replication-associated recombination protein A | - |
| NSU05_RS12955 (NSU05_12955) | - | 2530363..2531124 (-) | 762 | WP_250540521.1 | helix-turn-helix transcriptional regulator | - |
| NSU05_RS12960 (NSU05_12960) | - | 2531625..2532470 (+) | 846 | WP_038429340.1 | radical SAM protein | - |
| NSU05_RS12965 (NSU05_12965) | - | 2532521..2533117 (+) | 597 | WP_038429341.1 | 3'-5' exonuclease | - |
| NSU05_RS12970 (NSU05_12970) | - | 2533412..2534875 (+) | 1464 | WP_052124609.1 | carboxylesterase family protein | - |
| NSU05_RS12975 (NSU05_12975) | - | 2535226..2536353 (-) | 1128 | WP_038429342.1 | Rap family tetratricopeptide repeat protein | - |
| NSU05_RS12980 (NSU05_12980) | - | 2536539..2538464 (+) | 1926 | WP_038429343.1 | T7SS effector LXG polymorphic toxin | - |
| NSU05_RS12985 (NSU05_12985) | - | 2538478..2538765 (+) | 288 | WP_038429344.1 | hypothetical protein | - |
| NSU05_RS12990 (NSU05_12990) | - | 2539069..2539155 (+) | 87 | WP_072176383.1 | hypothetical protein | - |
| NSU05_RS12995 (NSU05_12995) | - | 2539168..2539608 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| NSU05_RS13000 (NSU05_13000) | - | 2539707..2540159 (+) | 453 | WP_038429345.1 | SMI1/KNR4 family protein | - |
| NSU05_RS13005 (NSU05_13005) | cdiI | 2540256..2540615 (+) | 360 | WP_038429346.1 | ribonuclease toxin immunity protein CdiI | - |
| NSU05_RS13010 (NSU05_13010) | - | 2540668..2541003 (+) | 336 | WP_038429347.1 | hypothetical protein | - |
| NSU05_RS13015 (NSU05_13015) | - | 2541156..2541572 (-) | 417 | WP_038429348.1 | hypothetical protein | - |
| NSU05_RS13020 (NSU05_13020) | - | 2541691..2542520 (-) | 830 | Protein_2515 | N-acetylmuramoyl-L-alanine amidase | - |
| NSU05_RS13025 (NSU05_13025) | - | 2542566..2542706 (-) | 141 | Protein_2516 | phage holin family protein | - |
| NSU05_RS13030 (NSU05_13030) | - | 2542733..2542918 (-) | 186 | Protein_2517 | phage terminase large subunit | - |
| NSU05_RS13035 (NSU05_13035) | terS | 2542915..2543623 (-) | 709 | Protein_2518 | phage terminase small subunit | - |
| NSU05_RS13040 (NSU05_13040) | - | 2543637..2543705 (-) | 69 | WP_250540529.1 | phage terminase small subunit-related protein | - |
| NSU05_RS13045 (NSU05_13045) | - | 2543887..2543973 (+) | 87 | WP_073991391.1 | YjcZ family sporulation protein | - |
| NSU05_RS13050 (NSU05_13050) | - | 2544014..2544106 (+) | 93 | WP_080019512.1 | YjcZ family sporulation protein | - |
| NSU05_RS13055 (NSU05_13055) | cotD | 2544292..2544507 (-) | 216 | WP_076458314.1 | spore coat protein CotD | - |
| NSU05_RS13060 (NSU05_13060) | - | 2545064..2545474 (-) | 411 | WP_052124610.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| NSU05_RS13065 (NSU05_13065) | - | 2545772..2546038 (+) | 267 | WP_029318115.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14891.88 Da Isoelectric Point: 5.1854
>NTDB_id=965097 NSU05_RS12905 WP_038429332.1 2521486..2521896(-) (nucA/comI) [Bacillus sp. EU52]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSSPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSSPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=965097 NSU05_RS12905 WP_038429332.1 2521486..2521896(-) (nucA/comI) [Bacillus sp. EU52]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTCCCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTCCCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
61.739 |
84.559 |
0.522 |