Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACJED3_RS20535 Genome accession   NZ_CP174155
Coordinates   3867218..3867628 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain G01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3862218..3872628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJED3_RS20510 - 3863323..3864213 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  ACJED3_RS20515 fumC 3864380..3865768 (+) 1389 WP_014480325.1 class II fumarate hydratase -
  ACJED3_RS20520 - 3865987..3866199 (+) 213 Protein_3982 recombinase family protein -
  ACJED3_RS20525 - 3866196..3866294 (-) 99 WP_031600702.1 hypothetical protein -
  ACJED3_RS20530 sigK 3866294..3867022 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ACJED3_RS20535 nucA/comI 3867218..3867628 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACJED3_RS20540 yqeB 3867661..3868383 (-) 723 WP_014480321.1 hypothetical protein -
  ACJED3_RS20545 gnd 3868634..3869527 (+) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACJED3_RS20550 yqeD 3869546..3870172 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  ACJED3_RS20555 cwlH 3870359..3871111 (+) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACJED3_RS20560 yqeF 3871363..3872094 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ACJED3_RS20565 - 3872400..3872540 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=962032 ACJED3_RS20535 WP_009967785.1 3867218..3867628(+) (nucA/comI) [Bacillus subtilis strain G01]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=962032 ACJED3_RS20535 WP_009967785.1 3867218..3867628(+) (nucA/comI) [Bacillus subtilis strain G01]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529