Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACF2JY_RS07655 Genome accession   NZ_CP172966
Coordinates   1456842..1457252 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain cel     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1451842..1462252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF2JY_RS07620 (ACF2JY_07620) arsR 1452007..1452324 (+) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -
  ACF2JY_RS07625 (ACF2JY_07625) arsK 1452383..1452823 (+) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ACF2JY_RS07630 (ACF2JY_07630) acr3 1452846..1453886 (+) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  ACF2JY_RS07635 (ACF2JY_07635) arsC 1453898..1454317 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ACF2JY_RS07640 (ACF2JY_07640) - 1454672..1454850 (+) 179 Protein_1498 hypothetical protein -
  ACF2JY_RS07645 (ACF2JY_07645) spoIVCA 1454808..1456268 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ACF2JY_RS07650 (ACF2JY_07650) - 1456296..1456646 (-) 351 Protein_1500 sigma-70 family RNA polymerase sigma factor -
  ACF2JY_RS07655 (ACF2JY_07655) nucA/comI 1456842..1457252 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACF2JY_RS07660 (ACF2JY_07660) yqeB 1457285..1458007 (-) 723 WP_046381199.1 hypothetical protein -
  ACF2JY_RS07665 (ACF2JY_07665) gnd 1458259..1459152 (+) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACF2JY_RS07670 (ACF2JY_07670) yqeD 1459171..1459797 (-) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  ACF2JY_RS07675 (ACF2JY_07675) cwlH 1459984..1460736 (+) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACF2JY_RS07680 (ACF2JY_07680) yqeF 1460988..1461719 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ACF2JY_RS07685 (ACF2JY_07685) - 1462025..1462165 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=956102 ACF2JY_RS07655 WP_009967785.1 1456842..1457252(+) (nucA/comI) [Bacillus subtilis strain cel]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=956102 ACF2JY_RS07655 WP_009967785.1 1456842..1457252(+) (nucA/comI) [Bacillus subtilis strain cel]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529