Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACFELJ_RS27495 Genome accession   NZ_CP172859
Coordinates   5905980..5906603 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 238629E_ID006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5900980..5911603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELJ_RS27475 (ACFELJ_27475) aroB 5901575..5902681 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  ACFELJ_RS27480 (ACFELJ_27480) aroK 5902731..5903249 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACFELJ_RS27485 (ACFELJ_27485) pilQ 5903261..5905405 (-) 2145 WP_400637398.1 type 4a pilus secretin PilQ Machinery gene
  ACFELJ_RS27490 (ACFELJ_27490) comL/pilP 5905459..5905983 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACFELJ_RS27495 (ACFELJ_27495) pilO 5905980..5906603 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFELJ_RS27500 (ACFELJ_27500) pilN 5906600..5907196 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACFELJ_RS27505 (ACFELJ_27505) pilM 5907196..5908260 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACFELJ_RS27510 (ACFELJ_27510) - 5908445..5910916 (+) 2472 WP_033967771.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=955673 ACFELJ_RS27495 WP_003103268.1 5905980..5906603(-) (pilO) [Pseudomonas aeruginosa strain 238629E_ID006]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=955673 ACFELJ_RS27495 WP_003103268.1 5905980..5906603(-) (pilO) [Pseudomonas aeruginosa strain 238629E_ID006]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCTGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401