Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V7S31_RS10180 Genome accession   NZ_CP147655
Coordinates   2076179..2076613 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain M1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2071179..2081613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7S31_RS10175 - 2074679..2075890 (-) 1212 WP_007409852.1 cytochrome P450 -
  V7S31_RS10180 nucA/comI 2076179..2076613 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  V7S31_RS10185 - 2076673..2077428 (+) 756 WP_003154084.1 YoaK family protein -
  V7S31_RS10190 - 2077462..2077824 (-) 363 WP_007410383.1 hypothetical protein -
  V7S31_RS10195 - 2078017..2079345 (-) 1329 WP_039063127.1 S8 family peptidase -
  V7S31_RS10200 - 2079524..2079757 (+) 234 WP_015239897.1 hypothetical protein -
  V7S31_RS10205 - 2080013..2080720 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  V7S31_RS10210 - 2080780..2081232 (+) 453 WP_007410380.1 OsmC family protein -
  V7S31_RS10215 - 2081246..2081599 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=947865 V7S31_RS10180 WP_007611576.1 2076179..2076613(+) (nucA/comI) [Bacillus velezensis strain M1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=947865 V7S31_RS10180 WP_007611576.1 2076179..2076613(+) (nucA/comI) [Bacillus velezensis strain M1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGAGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486