Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACFXAC_RS07775 Genome accession   NZ_CP170720
Coordinates   1562375..1564318 (+) Length   647 a.a.
NCBI ID   WP_012117534.1    Uniprot ID   A7Z4J7
Organism   Bacillus amyloliquefaciens strain JP5006     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1557375..1569318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAC_RS07750 (ACFXAC_07750) def 1557749..1558231 (+) 483 WP_014417743.1 peptide deformylase -
  ACFXAC_RS07755 (ACFXAC_07755) fmt 1558236..1559189 (+) 954 WP_014417744.1 methionyl-tRNA formyltransferase -
  ACFXAC_RS07760 (ACFXAC_07760) rsmB 1559176..1560519 (+) 1344 WP_014417745.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACFXAC_RS07765 (ACFXAC_07765) rlmN 1560523..1561614 (+) 1092 WP_053573429.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACFXAC_RS07770 (ACFXAC_07770) - 1561620..1562381 (+) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACFXAC_RS07775 (ACFXAC_07775) stkP 1562375..1564318 (+) 1944 WP_012117534.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ACFXAC_RS07780 (ACFXAC_07780) rsgA 1564333..1565223 (+) 891 WP_033574582.1 ribosome small subunit-dependent GTPase A -
  ACFXAC_RS07785 (ACFXAC_07785) rpe 1565225..1565875 (+) 651 WP_014417749.1 ribulose-phosphate 3-epimerase -
  ACFXAC_RS07790 (ACFXAC_07790) - 1565944..1566588 (+) 645 WP_053573430.1 thiamine diphosphokinase -
  ACFXAC_RS07795 (ACFXAC_07795) spoVM 1566675..1566755 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  ACFXAC_RS07800 (ACFXAC_07800) rpmB 1566833..1567021 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  ACFXAC_RS07805 (ACFXAC_07805) - 1567280..1567642 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71694.28 Da        Isoelectric Point: 5.0248

>NTDB_id=945330 ACFXAC_RS07775 WP_012117534.1 1562375..1564318(+) (stkP) [Bacillus amyloliquefaciens strain JP5006]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=945330 ACFXAC_RS07775 WP_012117534.1 1562375..1564318(+) (stkP) [Bacillus amyloliquefaciens strain JP5006]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATTCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCAGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTGTTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGATATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGGCGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAAAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCCTCAGCCGGCACCGATATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCTTTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366
  pknB Streptococcus mutans UA159

40.838

88.563

0.362