Detailed information NTG0093879

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OO714_RS14000
• Genome accession: NZ_AP025740
• Coordinates: 2974156..2975274 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain ATCC 19606
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2969156..2980274

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OO714_RS13990 (KAMO5_27840)	-	2972272..2972964 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
OO714_RS13995 (KAMO5_27850)	pilT	2973091..2974128 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OO714_RS14000 (KAMO5_27860)	pilU	2974156..2975274 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OO714_RS14005 (KAMO5_27870)	fur	2975344..2975781 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OO714_RS14010 (KAMO5_27880)	-	2975893..2976291 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OO714_RS14015 (KAMO5_27890)	-	2976346..2976669 (-)	324	WP_000442584.1	RnfH family protein	-
OO714_RS14020 (KAMO5_27900)	-	2976669..2977736 (-)	1068	WP_005144332.1	hypothetical protein	-
OO714_RS14025 (KAMO5_27910)	-	2977889..2978344 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OO714_RS14030 (KAMO5_27920)	-	2978597..2979445 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=93879 OO714_RS14000 WP_000347036.1 2974156..2975274(+) (pilU) [Acinetobacter baumannii strain ATCC 19606]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=93879 OO714_RS14000 WP_000347036.1 2974156..2975274(+) (pilU) [Acinetobacter baumannii strain ATCC 19606]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392