Detailed information NTG0927587

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VP466_RS17990
• Genome accession: NZ_CP142895
• Coordinates: 3728536..3729654 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain B20AB10
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3723536..3734654

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP466_RS17980 (VP466_18010)	-	3726652..3727344 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
VP466_RS17985 (VP466_18015)	pilT	3727471..3728508 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
VP466_RS17990 (VP466_18020)	pilU	3728536..3729654 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VP466_RS17995 (VP466_18025)	fur	3729724..3730161 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
VP466_RS18000 (VP466_18030)	-	3730273..3730671 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
VP466_RS18005 (VP466_18035)	-	3730726..3731049 (-)	324	WP_000442587.1	RnfH family protein	-
VP466_RS18010 (VP466_18040)	-	3731049..3732116 (-)	1068	WP_002019572.1	hypothetical protein	-
VP466_RS18015 (VP466_18045)	-	3732269..3732724 (-)	456	WP_000782976.1	bacteriohemerythrin	-
VP466_RS18020 (VP466_18050)	-	3732977..3733825 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=927587 VP466_RS17990 WP_000347039.1 3728536..3729654(+) (pilU) [Acinetobacter baumannii strain B20AB10]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=927587 VP466_RS17990 WP_000347039.1 3728536..3729654(+) (pilU) [Acinetobacter baumannii strain B20AB10]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395