Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VP456_RS13575 Genome accession   NZ_CP142142
Coordinates   2704072..2705109 (-) Length   345 a.a.
NCBI ID   WP_014418377.1    Uniprot ID   -
Organism   Bacillus velezensis strain PD9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2699072..2710109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP456_RS13525 (VP456_13490) tasA 2699305..2700090 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  VP456_RS13530 (VP456_13495) sipW 2700155..2700739 (-) 585 WP_012117977.1 signal peptidase I SipW -
  VP456_RS13535 (VP456_13500) tapA 2700711..2701382 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  VP456_RS13540 (VP456_13505) - 2701641..2701970 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  VP456_RS13545 (VP456_13510) - 2702011..2702190 (-) 180 WP_003153093.1 YqzE family protein -
  VP456_RS13550 (VP456_13515) comGG 2702247..2702624 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  VP456_RS13555 (VP456_13520) comGF 2702625..2703089 (-) 465 WP_233717055.1 competence type IV pilus minor pilin ComGF -
  VP456_RS13560 (VP456_13525) comGE 2703034..2703348 (-) 315 WP_029973875.1 competence type IV pilus minor pilin ComGE Machinery gene
  VP456_RS13565 (VP456_13530) comGD 2703332..2703769 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  VP456_RS13570 (VP456_13535) comGC 2703759..2704067 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  VP456_RS13575 (VP456_13540) comGB 2704072..2705109 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  VP456_RS13580 (VP456_13545) comGA 2705096..2706166 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  VP456_RS13585 (VP456_13550) - 2706359..2707309 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  VP456_RS13590 (VP456_13555) - 2707455..2708756 (+) 1302 WP_029973873.1 hemolysin family protein -
  VP456_RS13595 (VP456_13560) - 2708794..2709624 (-) 831 WP_025649847.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39600.91 Da        Isoelectric Point: 9.6787

>NTDB_id=921932 VP456_RS13575 WP_014418377.1 2704072..2705109(-) (comGB) [Bacillus velezensis strain PD9]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=921932 VP456_RS13575 WP_014418377.1 2704072..2705109(-) (comGB) [Bacillus velezensis strain PD9]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GACGGTTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TCATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGCTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

66.254

93.623

0.62