Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   VKS18_RS08135 Genome accession   NZ_CP141895
Coordinates   1607903..1608802 (+) Length   299 a.a.
NCBI ID   WP_032874887.1    Uniprot ID   -
Organism   Bacillus velezensis strain B004     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1602903..1613802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKS18_RS08115 (VKS18_17540) - 1603593..1605296 (+) 1704 WP_032874885.1 hypothetical protein -
  VKS18_RS08120 (VKS18_17545) - 1605293..1605574 (+) 282 WP_007409769.1 FlhB-like flagellar biosynthesis protein -
  VKS18_RS08125 (VKS18_17550) sucC 1605749..1606906 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  VKS18_RS08130 (VKS18_17555) sucD 1606935..1607837 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  VKS18_RS08135 (VKS18_17560) dprA 1607903..1608802 (+) 900 WP_032874887.1 DNA-processing protein DprA Machinery gene
  VKS18_RS08140 (VKS18_17565) topA 1608984..1611059 (+) 2076 WP_029325907.1 type I DNA topoisomerase -
  VKS18_RS08145 (VKS18_17570) trmFO 1611124..1612431 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  VKS18_RS08150 (VKS18_17575) xerC 1612501..1613418 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32853.99 Da        Isoelectric Point: 8.1570

>NTDB_id=920359 VKS18_RS08135 WP_032874887.1 1607903..1608802(+) (dprA) [Bacillus velezensis strain B004]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKNSELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAVGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=920359 VKS18_RS08135 WP_032874887.1 1607903..1608802(+) (dprA) [Bacillus velezensis strain B004]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGAATTCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCGTCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGTTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCTCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

42.045

88.294

0.371