Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABMW40_RS25040 Genome accession   NZ_CP158049
Coordinates   5358803..5359270 (-) Length   155 a.a.
NCBI ID   WP_003161762.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2-216     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5353803..5364270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABMW40_RS25005 (ABMW40_24995) - 5354017..5354385 (+) 369 WP_282831278.1 HNH endonuclease signature motif containing protein -
  ABMW40_RS25010 (ABMW40_25000) - 5354390..5354863 (+) 474 WP_282831276.1 TerS protein -
  ABMW40_RS25015 (ABMW40_25005) - 5354860..5356356 (+) 1497 WP_282831275.1 terminase TerL endonuclease subunit -
  ABMW40_RS25020 (ABMW40_25010) - 5356353..5356634 (+) 282 WP_282831274.1 head-tail connector protein -
  ABMW40_RS25025 (ABMW40_25015) - 5356634..5356954 (+) 321 WP_282831435.1 phage head closure protein -
  ABMW40_RS25035 (ABMW40_25025) - 5357332..5358729 (-) 1398 WP_225319908.1 O-antigen ligase -
  ABMW40_RS25040 (ABMW40_25030) pilA/pilAI 5358803..5359270 (-) 468 WP_003161762.1 pilin Machinery gene
  ABMW40_RS25045 (ABMW40_25035) pilB 5359501..5361201 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABMW40_RS25050 (ABMW40_25040) pilC 5361205..5362422 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  ABMW40_RS25055 (ABMW40_25045) pilD 5362423..5363295 (+) 873 WP_003161765.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABMW40_RS25060 (ABMW40_25050) coaE 5363292..5363903 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ABMW40_RS25065 (ABMW40_25055) yacG 5363900..5364100 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15959.30 Da        Isoelectric Point: 9.0145

>NTDB_id=907103 ABMW40_RS25040 WP_003161762.1 5358803..5359270(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2-216]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=907103 ABMW40_RS25040 WP_003161762.1 5358803..5359270(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2-216]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.679

100

0.477
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.439
  pilA Acinetobacter baumannii strain A118

43.312

100

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

37.427

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.262

90.968

0.394
  pilA Vibrio cholerae strain A1552

43.262

90.968

0.394
  pilA Vibrio cholerae C6706

43.262

90.968

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.333

96.774

0.381
  pilA/pilA1 Eikenella corrodens VA1

38.095

94.839

0.361