Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABL390_RS22225 Genome accession   NZ_CP157581
Coordinates   4834513..4835136 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NPA09B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4829513..4840136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL390_RS22210 (ABL390_22210) - 4830200..4832671 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  ABL390_RS22215 (ABL390_22215) pilM 4832856..4833920 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ABL390_RS22220 (ABL390_22220) pilN 4833920..4834516 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ABL390_RS22225 (ABL390_22225) pilO 4834513..4835136 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABL390_RS22230 (ABL390_22230) comL/pilP 4835133..4835657 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ABL390_RS22235 (ABL390_22235) pilQ 4835711..4837855 (+) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  ABL390_RS22240 (ABL390_22240) aroK 4837867..4838385 (+) 519 WP_003095833.1 shikimate kinase AroK -
  ABL390_RS22245 (ABL390_22245) aroB 4838435..4839541 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=906172 ABL390_RS22225 WP_003103268.1 4834513..4835136(+) (pilO) [Pseudomonas aeruginosa strain NPA09B]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=906172 ABL390_RS22225 WP_003103268.1 4834513..4835136(+) (pilO) [Pseudomonas aeruginosa strain NPA09B]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401