Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SIC15_RS13465 Genome accession   NZ_CP138917
Coordinates   2944473..2946188 (+) Length   571 a.a.
NCBI ID   WP_412536162.1    Uniprot ID   -
Organism   Marinobacter sp. MIT932201     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2939473..2951188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIC15_RS13445 - 2939829..2941190 (+) 1362 WP_412536158.1 MBL fold metallo-hydrolase -
  SIC15_RS13450 - 2941194..2941640 (-) 447 WP_412536159.1 pilin -
  SIC15_RS13455 - 2941772..2943625 (-) 1854 WP_412536160.1 O-antigen ligase family protein -
  SIC15_RS13460 - 2943625..2944062 (-) 438 WP_412536161.1 pilin -
  SIC15_RS13465 pilB 2944473..2946188 (+) 1716 WP_412536162.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SIC15_RS13470 pilC 2946191..2947411 (+) 1221 WP_412536163.1 type II secretion system F family protein Machinery gene
  SIC15_RS13475 pilD 2947504..2948379 (+) 876 WP_412536164.1 prepilin peptidase Machinery gene
  SIC15_RS13480 coaE 2948455..2949054 (+) 600 WP_412536165.1 dephospho-CoA kinase -
  SIC15_RS13485 - 2949047..2950141 (+) 1095 WP_412536166.1 hypothetical protein -
  SIC15_RS13490 tsaA 2950195..2950938 (+) 744 WP_412536167.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62903.99 Da        Isoelectric Point: 4.8615

>NTDB_id=905888 SIC15_RS13465 WP_412536162.1 2944473..2946188(+) (pilB) [Marinobacter sp. MIT932201]
MSTNKSNITLTGLARRFVDDGLLDEATAKDAFLQASQNRIALITYLTQNNLADSSKLAFSAAMEFGVSVLDLSAFMPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFITVSDPTNIQALDEIKFNTGLSTDAVLVDDAKLREAIDKYLESQDTSMGDLEDA
DLEGVETEGGEQEDEGTVTASEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQMYLNALQ
QPQGMILVTGPTGSGKTVSLYTGLNILNTADINISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSACKQPFEVPKDVLLEE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQSALMKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=905888 SIC15_RS13465 WP_412536162.1 2944473..2946188(+) (pilB) [Marinobacter sp. MIT932201]
ATGTCTACCAACAAAAGCAACATCACGCTCACGGGCCTTGCGAGGCGCTTCGTAGATGACGGTCTTCTGGATGAAGCAAC
GGCTAAAGATGCCTTTTTGCAAGCATCCCAGAACCGTATTGCCTTAATCACCTACCTGACCCAGAACAATCTTGCTGACA
GCTCAAAGCTTGCATTCTCGGCAGCGATGGAATTCGGCGTCTCTGTTCTGGACCTTTCAGCGTTTATGCCGGAAATGATG
CCGGAGAAGGTAGTAGATGAGAAGCTGGTCCGTAAGCACAACGCTCTTCCTTTATATAAGAGAGGTAATCGCCTGTTCAT
CACTGTCTCGGATCCAACTAATATCCAGGCTCTTGATGAAATCAAGTTCAATACAGGACTTAGTACCGATGCTGTCTTGG
TAGACGACGCCAAGCTCAGAGAAGCGATCGATAAATACCTGGAATCTCAGGATACCTCCATGGGTGATCTTGAAGATGCC
GATCTTGAAGGTGTTGAAACGGAAGGTGGAGAACAGGAAGACGAAGGAACAGTAACGGCAAGCGAAGTTGATGATGCACC
CATCGTTAAGTACGTAAACAAAATGCTGCTTGATGCAATTCGGGGCGGGGCGTCAGACATCCACTTTGAACCTTACGAAA
AGATCTATCGGGTTCGATATCGTACTGACGGTATCTTGAAAGAAGTTTCTCGGCCTTCTATAAAGCTAGCCCCAAAAATA
TCCGCCCGCGTAAAAATCATGGCCCAACTCGATATTTCCGAGAGACGAGTCCCCCAGGATGGCCGGATAAAAATGAAGCT
ATCCAAAACAAAAGCCATAGATTTCCGAGTCAACACGCTCCCCACCCTATGGGGTGAGAAGATCGTTTTGCGGATTCTTG
ATCCCAGTCAAGCGAAGCTTGGCATTGATGTTCTGGGTTATGAAGAAGATCAGAAGCAGATGTACCTGAATGCCCTGCAG
CAGCCACAAGGCATGATCCTCGTTACCGGGCCAACCGGCTCCGGCAAGACGGTTTCTCTGTATACCGGGCTGAACATACT
AAACACCGCGGACATCAATATCTCTACTGCCGAAGACCCTGCAGAAATCAACCTAGAGGGCATTAACCAAGTCAACGTGA
ACACGAGGGTTGGACTTGGCTTTGCTGAAGCACTCCGAGCGTTCCTGCGCCAGGATCCCGATGTAATCATGGTCGGTGAG
ATTCGGGATCTGGAAACGGCCAATATTGCTATCAAAGCGGCACAGACCGGGCACCTGGTTCTCTCCACCCTCCACACAAA
CAGTGCAGCAGAGACGCTCACCCGTATGATGAATATGGGCGTACCCGCGTTCAACATCGCTACTTCGGTAAGCCTGATTA
TTGCCCAACGCCTGGGCCGAAGACTGTGCAGCGCTTGCAAACAGCCCTTCGAAGTCCCGAAAGACGTGCTGTTAGAGGAG
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGTCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
AGGCCGCGTCGGGATTTACGAGGTGGTCAAAATTACCGACGAGCTGGCGAACATGATTATGGAAGAAGCCAGTTCCATTA
AAATTGCAAAGCAGGCTCAGGCCGAGGGCTTCCGTAACTTGCGTCAATCGGCACTTATGAAAGTGATCGAGGGCGTAACA
AGCCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.068

100

0.573
  pilB Acinetobacter baylyi ADP1

55.944

100

0.56
  pilB Legionella pneumophila strain ERS1305867

53.887

99.124

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

51.346

97.548

0.501
  pilB Vibrio campbellii strain DS40M4

50.355

98.774

0.497
  pilB Vibrio cholerae strain A1552

55.146

90.193

0.497
  pilF Neisseria gonorrhoeae MS11

48.135

98.599

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.734

92.119

0.366