Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   SBK94_RS08450 Genome accession   NZ_CP137889
Coordinates   1643973..1644872 (+) Length   299 a.a.
NCBI ID   WP_014305001.1    Uniprot ID   -
Organism   Bacillus velezensis strain N23     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1638973..1649872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBK94_RS08430 (SBK94_08430) - 1639664..1641367 (+) 1704 WP_014305000.1 hypothetical protein -
  SBK94_RS08435 (SBK94_08435) - 1641364..1641645 (+) 282 WP_003154284.1 FlhB-like flagellar biosynthesis protein -
  SBK94_RS08440 (SBK94_08440) sucC 1641820..1642977 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  SBK94_RS08445 (SBK94_08445) sucD 1643006..1643908 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  SBK94_RS08450 (SBK94_08450) dprA 1643973..1644872 (+) 900 WP_014305001.1 DNA-processing protein DprA Machinery gene
  SBK94_RS08455 (SBK94_08455) topA 1645054..1647129 (+) 2076 WP_003154274.1 type I DNA topoisomerase -
  SBK94_RS08460 (SBK94_08460) trmFO 1647194..1648501 (+) 1308 WP_003154273.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  SBK94_RS08465 (SBK94_08465) xerC 1648571..1649488 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32920.05 Da        Isoelectric Point: 8.1570

>NTDB_id=901099 SBK94_RS08450 WP_014305001.1 1643973..1644872(+) (dprA) [Bacillus velezensis strain N23]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTPLLEKGRKIGIVGTRKPTEDGIKAARHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=901099 SBK94_RS08450 WP_014305001.1 1643973..1644872(+) (dprA) [Bacillus velezensis strain N23]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTACCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
ACCGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTAGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGTTTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

41.288

88.294

0.365
  dprA Legionella pneumophila strain ERS1305867

43.548

82.943

0.361