Detailed information NTG0089493

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: LDO10_RS00100
• Genome accession: NZ_AP024929
• Coordinates: 18943..21609 (-)
• Length: 888 a.a.
• NCBI ID: WP_223968753.1
• Uniprot ID: A0AAD1KSD1
• Organism: Thermus thermophilus strain AA2-2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13943..26609

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO10_RS00080 (TthAA22_00120)	-	14789..15421 (-)	633	WP_143584081.1	histidine phosphatase family protein	-
LDO10_RS00085 (TthAA22_00130)	purM	15418..16419 (-)	1002	WP_143584083.1	phosphoribosylformylglycinamidine cyclo-ligase	-
LDO10_RS00090 (TthAA22_00140)	gatB	16425..17834 (-)	1410	WP_143584085.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LDO10_RS00095 (TthAA22_00150)	pilT	17845..18930 (-)	1086	WP_143584087.1	type IV pilus twitching motility protein PilT	Machinery gene
LDO10_RS00100 (TthAA22_00160)	pilF	18943..21609 (-)	2667	WP_223968753.1	type IV pilus assembly ATPase PilB	Machinery gene
LDO10_RS00105 (TthAA22_00170)	-	21606..22091 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
LDO10_RS00110 (TthAA22_00180)	pgeF	22126..22854 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
LDO10_RS00115 (TthAA22_00190)	surE	22851..23606 (-)	756	WP_014628820.1	5'/3'-nucleotidase SurE	-
LDO10_RS00120 (TthAA22_00200)	-	23665..23871 (-)	207	WP_143584091.1	cold-shock protein	-
LDO10_RS00125 (TthAA22_00210)	-	23971..24618 (-)	648	WP_223966705.1	transposase	-
LDO10_RS00130	-	24560..24745 (+)	186	WP_223966770.1	hypothetical protein	-
LDO10_RS00135 (TthAA22_00230)	-	25064..26353 (+)	1290	WP_143584093.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97972.02 Da
• Isoelectric Point: 5.3309

>NTDB_id=89493 LDO10_RS00100 WP_223968753.1 18943..21609(-) (pilF) [Thermus thermophilus strain AA2-2]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAAALL
LRSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRQGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQEEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVIS
VVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLS
EAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=89493 LDO10_RS00100 WP_223968753.1 18943..21609(-) (pilF) [Thermus thermophilus strain AA2-2]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTTGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTGTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAAAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCAGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGA
ACCACGAGGTGGCCCCCGCTGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGAGGAGGAGGCCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCA
GAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACGACG
TCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCC
GTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGC
CATCGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCT
CGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACAGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCGCAGG
CGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTCAACATCTCCGCTGCCCTCATCGGCGTCCTCTCCCAGC
GCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCC
GAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	98.875	100	0.99
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.955	100	0.561