Detailed information NTG0885745

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RQL82_RS13755
• Genome accession: NZ_CP135159
• Coordinates: 2917207..2918325 (+)
• Length: 372 a.a.
• NCBI ID: WP_005070855.1
• Uniprot ID: A0A013SVN8
• Organism: Acinetobacter pittii strain AR3651
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2912207..2923325

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RQL82_RS13745 (RQL82_13805)	yggS	2915331..2916023 (-)	693	WP_329759705.1	YggS family pyridoxal phosphate-dependent enzyme	-
RQL82_RS13750 (RQL82_13810)	pilT	2916150..2917187 (+)	1038	WP_262441968.1	type IV pilus twitching motility protein PilT	Machinery gene
RQL82_RS13755 (RQL82_13815)	pilU	2917207..2918325 (+)	1119	WP_005070855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RQL82_RS13760 (RQL82_13820)	fur	2918395..2918832 (-)	438	WP_016141990.1	ferric iron uptake transcriptional regulator	-
RQL82_RS13765 (RQL82_13825)	omlA	2918944..2919342 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
RQL82_RS13770 (RQL82_13830)	-	2919407..2919721 (-)	315	WP_329759707.1	RnfH family protein	-
RQL82_RS13775 (RQL82_13835)	-	2919721..2920788 (-)	1068	WP_329759708.1	hypothetical protein	-
RQL82_RS13780 (RQL82_13840)	-	2920942..2921397 (-)	456	WP_002121698.1	bacteriohemerythrin	-
RQL82_RS13785 (RQL82_13845)	-	2921649..2922497 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=885745 RQL82_RS13755 WP_005070855.1 2917207..2918325(+) (pilU) [Acinetobacter pittii strain AR3651]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=885745 RQL82_RS13755 WP_005070855.1 2917207..2918325(+) (pilU) [Acinetobacter pittii strain AR3651]
ATGGATTTTAATGACCTACTCAACCTAATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTGCGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCATGAACACGCTGGCTGCATCATTACTCAACGTGAAGTCGGAATTGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACGCCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGATCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013SVN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363