Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF/cglF   Type   Machinery gene
Locus tag   RMP66_RS00815 Genome accession   NZ_CP135094
Coordinates   128661..129095 (+) Length   144 a.a.
NCBI ID   WP_029750050.1    Uniprot ID   -
Organism   Streptococcus suis strain 1547095     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 123661..134095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMP66_RS00780 (RMP66_00780) - 124403..124768 (+) 366 WP_002936568.1 DUF1033 family protein -
  RMP66_RS00785 (RMP66_00785) - 124803..125753 (-) 951 WP_024394700.1 S66 peptidase family protein -
  RMP66_RS00790 (RMP66_00790) comYA 125839..126789 (+) 951 WP_014637312.1 competence type IV pilus ATPase ComGA Machinery gene
  RMP66_RS00795 (RMP66_00795) comYB 126701..127738 (+) 1038 WP_043032925.1 competence type IV pilus assembly protein ComGB Machinery gene
  RMP66_RS00800 (RMP66_00800) comYC 127740..128021 (+) 282 WP_011921720.1 competence type IV pilus major pilin ComGC Machinery gene
  RMP66_RS00805 (RMP66_00805) comGD 128002..128409 (+) 408 WP_024394702.1 competence type IV pilus minor pilin ComGD -
  RMP66_RS00810 (RMP66_00810) comYE 128381..128674 (+) 294 WP_024394703.1 competence type IV pilus minor pilin ComGE Machinery gene
  RMP66_RS00815 (RMP66_00815) comGF/cglF 128661..129095 (+) 435 WP_029750050.1 competence type IV pilus minor pilin ComGF Machinery gene
  RMP66_RS00820 (RMP66_00820) comGG 129073..129483 (+) 411 WP_024394705.1 competence type IV pilus minor pilin ComGG -
  RMP66_RS00825 (RMP66_00825) comYH 129540..130493 (+) 954 WP_024394706.1 class I SAM-dependent methyltransferase Machinery gene
  RMP66_RS00830 (RMP66_00830) - 130543..131730 (+) 1188 WP_014637319.1 acetate kinase -
  RMP66_RS00835 (RMP66_00835) - 132045..132596 (+) 552 WP_014636756.1 folate family ECF transporter S component -
  RMP66_RS00840 (RMP66_00840) - 132650..133906 (+) 1257 WP_172005964.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence


Protein


Download         Length: 144 a.a.        Molecular weight: 16507.85 Da        Isoelectric Point: 8.8193

>NTDB_id=885073 RMP66_RS00815 WP_029750050.1 128661..129095(+) (comGF/cglF) [Streptococcus suis strain 1547095]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLIQEAHYQRQSVQKEWLVFSSQLRSEWDRSELVKVENGKVYVDKGG
QSLAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=885073 RMP66_RS00815 WP_029750050.1 128661..129095(+) (comGF/cglF) [Streptococcus suis strain 1547095]
TTGTTAAAAAGTAAAATTCCCGCTTTTACTCTCCTGGAATGCTTGGTAGCCTTAGTCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAGTTGTTGATTCAAGAAGCACATTACCAACGTCAATCTGTCCAAAAGGAGTGGCTGGTATTTT
CTAGTCAGTTGCGGTCAGAGTGGGACCGGTCGGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTTGATAAAGGTGGT
CAGTCGTTGGCCTTTGGTAAGTCACGATCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGCTACCAGCCCATGCT
CTATCAGGTGGACAGTGCAGCTATTTCCCAAGAAAACCAGCTGGTGCGTATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

56.738

97.917

0.556

  comGF/cglF Streptococcus pneumoniae Rx1

56.738

97.917

0.556

  comGF/cglF Streptococcus pneumoniae D39

56.738

97.917

0.556

  comGF/cglF Streptococcus pneumoniae R6

56.738

97.917

0.556

  comGF/cglF Streptococcus mitis NCTC 12261

57.463

93.056

0.535

  comGF/cglF Streptococcus mitis SK321

54.478

93.056

0.507

  comYF Streptococcus mutans UA140

50.694

100

0.507

  comYF Streptococcus mutans UA159

50

100

0.5

  comGF Lactococcus lactis subsp. cremoris KW2

46.099

97.917

0.451