Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RF668_RS04475 Genome accession   NZ_CP133787
Coordinates   868450..869811 (+) Length   453 a.a.
NCBI ID   WP_011677138.1    Uniprot ID   A0A165G707
Organism   Lactococcus cremoris strain KCKM 0438     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 863450..874811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF668_RS04450 (RF668_04450) - 863579..865102 (+) 1524 WP_309559315.1 polysaccharide transporter -
  RF668_RS04455 (RF668_04455) - 865132..866106 (+) 975 WP_043736198.1 GHMP kinase -
  RF668_RS04460 (RF668_04460) - 866168..866743 (+) 576 WP_043736199.1 SIS domain-containing protein -
  RF668_RS04465 (RF668_04465) - 866740..867285 (+) 546 WP_043736200.1 HAD-IIIA family hydrolase -
  RF668_RS04470 (RF668_04470) - 867267..868280 (+) 1014 WP_043736202.1 glycosyltransferase family 2 protein -
  RF668_RS04475 (RF668_04475) radA 868450..869811 (+) 1362 WP_011677138.1 DNA repair protein RadA Machinery gene
  RF668_RS04480 (RF668_04480) - 869857..870534 (-) 678 WP_021037928.1 YoaK family protein -
  RF668_RS04485 (RF668_04485) - 870801..871886 (+) 1086 WP_043736205.1 PIN/TRAM domain-containing protein -
  RF668_RS04490 (RF668_04490) - 871945..872574 (+) 630 WP_043736207.1 hypothetical protein -
  RF668_RS04495 (RF668_04495) - 872695..873051 (+) 357 WP_043736209.1 PadR family transcriptional regulator -
  RF668_RS04500 (RF668_04500) - 873044..873382 (+) 339 WP_043736212.1 DUF1048 domain-containing protein -
  RF668_RS04505 (RF668_04505) - 873379..873702 (+) 324 WP_021037923.1 DUF1048 domain-containing protein -
  RF668_RS04510 (RF668_04510) - 873793..874287 (-) 495 WP_309559319.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49897.33 Da        Isoelectric Point: 5.2965

>NTDB_id=876875 RF668_RS04475 WP_011677138.1 868450..869811(+) (radA) [Lactococcus cremoris strain KCKM 0438]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=876875 RF668_RS04475 WP_011677138.1 868450..869811(+) (radA) [Lactococcus cremoris strain KCKM 0438]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAGTCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGTTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGCTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCGACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACGAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACGATTATGACACCTGAAATTCAATCAACTCAAGGCTCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTAAAAAACCGGTTTGGGTCAACCAATGAAATTGGTATATTCGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATATTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTCAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGCTTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCGGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGA
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCGAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165G707

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623