Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RB215_RS12685 Genome accession   NZ_CP133571
Coordinates   2713256..2714392 (-) Length   378 a.a.
NCBI ID   WP_011329250.1    Uniprot ID   A0A1R4ISF5
Organism   Pseudoalteromonas sp. HL-AS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708256..2719392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB215_RS12655 (RB215_12655) - 2708777..2709670 (+) 894 WP_011329244.1 hypothetical protein -
  RB215_RS12660 (RB215_12660) - 2709738..2710217 (+) 480 WP_309216516.1 hypothetical protein -
  RB215_RS12665 (RB215_12665) rsmE 2710290..2711024 (+) 735 WP_011329246.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RB215_RS12670 (RB215_12670) gshB 2711079..2712029 (+) 951 WP_089368758.1 glutathione synthase -
  RB215_RS12675 (RB215_12675) - 2712170..2712727 (+) 558 WP_058374183.1 YqgE/AlgH family protein -
  RB215_RS12680 (RB215_12680) ruvX 2712740..2713189 (+) 450 WP_011329249.1 Holliday junction resolvase RuvX -
  RB215_RS12685 (RB215_12685) pilU 2713256..2714392 (-) 1137 WP_011329250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RB215_RS12690 (RB215_12690) pilT 2714402..2715442 (-) 1041 WP_041454575.1 type IV pilus twitching motility protein PilT Machinery gene
  RB215_RS12695 (RB215_12695) - 2715445..2716158 (+) 714 WP_011329252.1 YggS family pyridoxal phosphate-dependent enzyme -
  RB215_RS12700 (RB215_12700) proC 2716176..2716997 (+) 822 WP_011329253.1 pyrroline-5-carboxylate reductase -
  RB215_RS12705 (RB215_12705) - 2717010..2717543 (+) 534 WP_058374186.1 YggT family protein -
  RB215_RS12710 (RB215_12710) - 2717602..2718045 (+) 444 WP_182676591.1 DUF4426 domain-containing protein -
  RB215_RS12715 (RB215_12715) rdgB 2718053..2718652 (+) 600 WP_309216517.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41839.84 Da        Isoelectric Point: 5.8295

>NTDB_id=874992 RB215_RS12685 WP_011329250.1 2713256..2714392(-) (pilU) [Pseudoalteromonas sp. HL-AS2]
MALSLNNFLQIMIDKKGSDLFVSSQLPISAKINGELTPLDDEPLSDEQSLELVESAMSEKQKAEFHTTKECNFAIATDEG
RFRVSAFWQRDKAGMVIRRIVTKIPEIGELGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGQGRVAAIEILLNSPMVAELIKNGDIGGIKETMAKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSANDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIS

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=874992 RB215_RS12685 WP_011329250.1 2713256..2714392(-) (pilU) [Pseudoalteromonas sp. HL-AS2]
ATGGCTTTATCTTTAAATAACTTTTTACAAATAATGATAGATAAAAAAGGCTCTGATTTATTTGTTTCAAGCCAACTGCC
TATCAGCGCTAAAATTAATGGTGAATTAACCCCGCTTGATGATGAGCCGCTCAGCGATGAGCAATCACTTGAGTTGGTTG
AGTCGGCAATGAGTGAAAAACAAAAAGCCGAGTTTCATACAACGAAAGAATGCAACTTTGCGATTGCCACTGACGAAGGA
CGTTTTCGTGTTTCGGCATTTTGGCAGCGCGATAAAGCGGGCATGGTTATTCGTCGTATTGTTACTAAAATACCTGAAAT
TGGCGAACTTGGGCTGCCGTCTACACTTACCGATGTGATTATGTCGAAACGTGGTTTAGTGCTATTTGTGGGGGGAACCG
GTACGGGTAAGTCAACCTCACTTGCTGCACTTATTGGCTATAGAAACCGTAATCAGCGCGGACATATATTAACCATTGAA
GACCCGATTGAGTTTGTACACGAACACCGTAAAAGTATTATTACTCAACGCGAAGTAGGGCTAGATACCGAAAGTTTTGA
GTCAGCGCTTAAAAGCTCGTTACGCCAAGCACCCGACGTAATATTAATAGGCGAAATACGCTCACAAGAAACCATGGAGT
ATGCATTAAGCTTTGCAGAAACAGGGCACTTATGTGTGGCTACCTTGCATGCAAACAACGCTAACCAAGCGATAGATCGG
ATCATGCATTTAGTGCCAAAAGAAAAGCACGATAAGCTTAAGTACGATCTAGCATTAAATTTACGCGCTATTATTGCCCA
GCAGCTGATCCCCACATCTGACGGGCAAGGTCGAGTTGCTGCTATTGAAATATTACTTAATTCACCTATGGTTGCAGAGC
TAATTAAAAATGGTGATATAGGCGGTATTAAAGAAACCATGGCTAAATCGAAAGAAATGGGTATGCAGACCTTTGATCAG
GCCTTATTTGAGCTGTATAAACAGCAACGAATCAATTATGCAGATGCATTGCACCATGCCGATTCGGCGAACGATTTGCG
CTTAATGATAAAGCTGCGCAACAATGAGCAGCAAGGGGCGGGCTTTTTACAAGGGGTCACTATTGATGGCCTTGATGATA
AAGGCAATATTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R4ISF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Acinetobacter baylyi ADP1

56.764

99.735

0.566
  pilU Vibrio cholerae strain A1552

57.766

97.09

0.561
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.044

95.503

0.421