Detailed information    

insolico Bioinformatically predicted

Overview

Name   codY   Type   Regulator
Locus tag   RCF35_RS13335 Genome accession   NZ_CP133277
Coordinates   2623289..2624068 (+) Length   259 a.a.
NCBI ID   WP_003154263.1    Uniprot ID   I2C545
Organism   Bacillus velezensis strain NBNZ-0060     
Function   repression of comK (predicted from homology)   
Competence regulation

Genomic Context

Location: 2618289..2629068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS13315 (RCF35_13315) trmFO 2618976..2620283 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  RCF35_RS13320 (RCF35_13320) xerC 2620353..2621270 (+) 918 WP_007409774.1 tyrosine recombinase XerC -
  RCF35_RS13325 (RCF35_13325) hslV 2621286..2621831 (+) 546 WP_003154267.1 ATP-dependent protease subunit HslV -
  RCF35_RS13330 (RCF35_13330) hslU 2621848..2623251 (+) 1404 WP_007611413.1 HslU--HslV peptidase ATPase subunit -
  RCF35_RS13335 (RCF35_13335) codY 2623289..2624068 (+) 780 WP_003154263.1 GTP-sensing pleiotropic transcriptional regulator CodY Regulator
  RCF35_RS13340 (RCF35_13340) flgB 2624422..2624811 (+) 390 WP_003154260.1 flagellar basal body rod protein FlgB -
  RCF35_RS13345 (RCF35_13345) flgC 2624811..2625263 (+) 453 WP_007409776.1 flagellar basal body rod protein FlgC -
  RCF35_RS13350 (RCF35_13350) fliE 2625274..2625594 (+) 321 WP_007409777.1 flagellar hook-basal body complex protein FliE -
  RCF35_RS13355 (RCF35_13355) fliF 2625637..2627247 (+) 1611 WP_032874889.1 flagellar basal-body MS-ring/collar protein FliF -
  RCF35_RS13360 (RCF35_13360) fliG 2627260..2628276 (+) 1017 WP_003154256.1 flagellar motor switch protein FliG -
  RCF35_RS13365 (RCF35_13365) fliH 2628269..2629024 (+) 756 WP_032874891.1 flagellar assembly protein FliH -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29056.24 Da        Isoelectric Point: 4.6017

>NTDB_id=873218 RCF35_RS13335 WP_003154263.1 2623289..2624068(+) (codY) [Bacillus velezensis strain NBNZ-0060]
MALLQKTRIINSMLQAAAGKPVNFKEMAETLRDVIDSNIFVVSRRGKLLGYSINQQIENDRMKKMLEDRQFPEEYTRNLF
NVPETSSNLDINSEYTAFPVENRDLFQAGLTTIVPIIGGGERLGTLILSRLQDQFEDDDLILAEYGATVVGMEILREKAE
EIEEEARSKAVVQMAISSLSYSELEAIEHIFEELDGNEGLLVASKIADRVGITRSVIVNALRKLESAGVIESRSLGMKGT
YIKVLNNKFLIELENLKSH

Nucleotide


Download         Length: 780 bp        

>NTDB_id=873218 RCF35_RS13335 WP_003154263.1 2623289..2624068(+) (codY) [Bacillus velezensis strain NBNZ-0060]
ATGGCTTTACTACAAAAAACACGAATTATTAACAGCATGCTGCAGGCTGCGGCAGGCAAACCCGTAAACTTTAAAGAAAT
GGCTGAAACGCTTCGTGACGTCATTGATTCTAATATTTTCGTCGTCAGCCGCAGAGGAAAGCTTTTGGGCTACTCTATTA
ATCAGCAGATTGAAAATGACCGCATGAAAAAAATGCTTGAAGACCGCCAGTTCCCGGAAGAGTACACGAGAAATCTGTTC
AATGTTCCGGAGACGTCTTCAAACCTTGATATCAACAGCGAATACACGGCGTTTCCTGTAGAAAACAGAGACCTGTTCCA
AGCGGGCTTAACAACGATCGTGCCGATCATCGGCGGAGGAGAGAGACTGGGCACGCTCATTCTGTCCCGTCTGCAGGATC
AATTTGAAGATGATGATCTCATTCTTGCTGAATACGGAGCAACCGTTGTAGGAATGGAGATTTTAAGAGAAAAAGCGGAA
GAAATCGAAGAAGAGGCGCGAAGCAAAGCGGTTGTTCAAATGGCGATCAGTTCTCTGTCTTACAGTGAACTTGAAGCGAT
TGAGCATATCTTTGAAGAGCTTGACGGAAACGAAGGCTTGCTTGTGGCAAGTAAGATTGCGGACCGTGTCGGCATTACGC
GTTCAGTAATCGTTAACGCGCTGAGAAAGCTGGAAAGCGCAGGCGTCATTGAATCCAGATCACTGGGAATGAAGGGGACG
TACATTAAAGTTCTGAACAACAAATTCCTTATTGAATTGGAAAACCTTAAATCTCATTAA

Domains

Predicted by InterproScan.

CodY GAF-like domain (CodY)

(3-178)

CodY helix-turn-helix domain (HTH_CodY)

(198-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C545

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  codY Bacillus subtilis subsp. subtilis str. 168

99.228

100

0.992
  codY Lactococcus lactis subsp. lactis strain DGCC12653

47.843

98.456

0.471